The ABCA1 transporter functions on the basolateral surface of hepatocytes.

Abstract:

:ABCA1 on the cell surface and in endosomes plays an essential role in the cell-mediated lipidation of apoA-I to form nascent HDL. Our previous studies of transgenic mice overexpressing ABCA1 suggested that ABCA1 in the liver plays a major role in regulating plasma HDL levels. The site of function of ABCA1 in the polarized hepatocyte was currently assessed by expression of an adenoviral construct encoding a human ABCA1-GFP fusion protein in the polarized hepatocyte-like WIF-B cell line. Consistent with localization of ABCA1 at the basolateral (vascular) cell surface, expression of ABCA1-GFP stimulated apoA-I mediated efflux of WIF-B cell cholesterol into the culture medium. Confocal fluorescence microscopy revealed that ABCA1-GFP was expressed solely on the basolateral surface and associated endocytic vesicles. These findings suggest an important role for hepatocyte basolateral membrane ABCA1 in the regulation of the levels of intracellular hepatic cholesterol, as well as plasma HDL.

authors

Neufeld EB,Demosky SJ Jr,Stonik JA,Combs C,Remaley AT,Duverger N,Santamarina-Fojo S,Brewer HB Jr

doi

10.1016/s0006-291x(02)02274-x

subject

Has Abstract

pub_date

2002-10-04 00:00:00

pages

974-9

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006291X0202274X

journal_volume

297

pub_type

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