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Analysis of the Escherichia coli K-12 cysB gene and its product using the method of gene fusion.

Abstract：

:The amino-terminal structure and the essential functional region of the cysB gene product of Escherichia coli K-12 were analyzed by the method of gene fusion. The translational start codon of the cysB gene was located by determining the amino-terminal sequence of a hybrid protein containing the first 31 amino acid residues of the CysB protein at the amino terminus of beta-galactosidase(LacZ protein). The fact that two other CysB'-'LacZ hybrid polypeptides expressed a normal CysB activity indicated that the functional region of the CysB protein was located within the first 215 amino acid residues of the total 324 amino acids deduced from the nucleotide sequence.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Tei H,Watanabe K,Murata K,Kimura A

doi

10.1016/0006-291x(90)90617-v

subject

Has Abstract

pub_date

1990-03-30 00:00:00

pages

962-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(90)90617-V

journal_volume

167

pub_type

杂志文章

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