Analysis of the pancreatic beta cell in the mouse with targeted disruption of the pancreatic beta cell-specific glucokinase gene.

abstract：:In this report, we demonstrate that HL-60 nuclei isolated in calcium but not EGTA containing buffers specifically bind PE and express approximately 37,000 receptor sites/nucleus. Nuclear phorbol ester binding is lost by isolation in the absence of calcium, but can be repleted by the addition of partially purified prot...

journal_title：Biochemical and biophysical research communications

authors： Kraft AS,Appling C,Berkow RL

更新日期：1987-04-14 00:00:00

abstract：:Transforming growth factor (TGF)-beta1 can cause fibrosis diseases by enhancing production of collagen. However, the intracellular signaling mechanism for TGF-beta1 stimulation of this process has not been fully elucidated. The present study focused on this mechanism and the cross-talk between the MAPK and Smad pathwa...

journal_title：Biochemical and biophysical research communications

authors： Li F,Zeng B,Chai Y,Cai P,Fan C,Cheng T

更新日期：2009-08-21 00:00:00

abstract：:Ferroptosis is a form of regulated cell death resulting from iron accumulation and lipid peroxidation. While impaired ferroptosis is tightly linked to human diseases and conditions, the mechanism and regulation of ferroptosis remain largely unknown. Here, we demonstrate that STAT3 is a positive regulator of ferroptosi...

journal_title：Biochemical and biophysical research communications

authors： Gao H,Bai Y,Jia Y,Zhao Y,Kang R,Tang D,Dai E

更新日期：2018-09-10 00:00:00

abstract：:Simple polyesters derived from poly(ethylene glycol)s and alpha, omega-dicarboxylic acids exhibit a broad range of activity in disrupting phospholipid membranes. This activity has been analyzed by measuring the release of liposome-encapsulated 5(6)-carboxy-fluorescein (CF). Comparison with an analogous monomeric surfa...

journal_title：Biochemical and biophysical research communications

authors： Regen SL,Jayasuriya N,Fabianowski W

更新日期：1989-03-15 00:00:00

abstract：:In vertebrate cells, DNA double-strand breaks are efficiently repaired by homologous recombination or nonhomologous end-joining (NHEJ). The latter pathway relies on Ku (the Ku70/Ku86 heterodimer), DNA-PKcs, Artemis, Xrcc4, and DNA ligase IV (Lig4). Here, we show that a human pre-B cell line nullizygous for Lig4 exhibi...

journal_title：Biochemical and biophysical research communications

authors： Adachi N,Iiizumi S,So S,Koyama H

更新日期：2004-06-11 00:00:00

abstract：:HOX genes are transcription factors that play important roles in body patterning and many cellular processes during embryonic, fetal, and adult development. Given their important function in normal tissues, it is reasonable to assume that abnormal expression of HOX genes in adults could lead to serious diseases such a...

journal_title：Biochemical and biophysical research communications

authors： Lee JY,Kim JM,Jeong DS,Kim MH

更新日期：2018-09-18 00:00:00

abstract：:miRNA is increasingly being recognized as a key regulator of metabolism in animals. A wealth of evidence has suggested that miRNA mainly binds 3' UTR of mRNA and modulates the cell activities via repressing the mRNA translation. However, as the translation initiates at 5' UTR, cis elements like upstream open reading f...

journal_title：Biochemical and biophysical research communications

authors： Hu W,Wang T,Yang Y,Zheng S

更新日期：2014-09-12 00:00:00

abstract：:We previously demonstrated several nicotinic acetylcholine receptor (nAChR) subunits and associated proteins in human sperm. Here, we identified in sperm for the first time two additional nAChR-associated molecules: (1) agrin(SN)Z(+) in human sperm localized in the posterior post-acrosomal, neck, and flagellar mid-pie...

journal_title：Biochemical and biophysical research communications

authors： Kumar P,Ferns MJ,Meizel S

更新日期：2006-04-07 00:00:00

abstract：:1,2-Naphthoquinone (1,2-NQ) is an atmospheric contaminant with electrophilic properties that allow it to react readily with protein thiol groups such as those found on the cAMP response element-binding protein (CREB), a transcription factor with conserved cysteine residues that regulate DNA binding. In the present stu...

journal_title：Biochemical and biophysical research communications

authors： Endo A,Sumi D,Kumagai Y

更新日期：2007-09-14 00:00:00

abstract：:Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 microM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within ...

journal_title：Biochemical and biophysical research communications

authors： Grubb DR,Wolvetang EJ,Lawen A

更新日期：1995-10-24 00:00:00

abstract：:The human pregnane X receptor (hPXR) plays a key role in the regulation of both drug metabolism and efflux by inducing the expression of CYP3A4 and MDR1 gene. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we identified seven novel splicing variants of hPXR in tissue from a single human liver...

journal_title：Biochemical and biophysical research communications

authors： Fukuen S,Fukuda T,Matsuda H,Sumida A,Yamamoto I,Inaba T,Azuma J

更新日期：2002-11-01 00:00:00

abstract：:Little is known about the gene structure and mRNA expression of cholecystokinin type-A(CCKA) receptor in animals or in human. By screening an EMBL phage library containing partially digested Long-Evans rat DNA, we isolated 7 genomic clones covering a continuous 27 kb region over which a rat CCKA receptor gene spanned....

journal_title：Biochemical and biophysical research communications

authors： Takata Y,Takiguchi S,Funakoshi A,Kono A

更新日期：1995-08-24 00:00:00

abstract：:In some v-Ha-ras-transfected cell lines, serum deprivation results in apoptosis. Clarification of the molecular mechanisms by which oncogenic Ras controls susceptibility to apoptosis may assist in the development of effective therapies against human cancer with oncogenic ras gene. In this report, we established a v-Ha...

journal_title：Biochemical and biophysical research communications

authors： Arase Y,Hiwasa T,Hasegawa R,Nomura J,Ito H,Suzuki N

更新日期：2000-01-07 00:00:00

abstract：:The role of heteromeric phosphoinositide (PI) 3-kinase activity in insulin signal transduction was studied by investigating the effects of (i) overexpression of a dominant negative mutant p85 (delta p85) that lacks the binding site for p110 (delta p85-overexpressing cells) and (ii) inhibition of PI 3-kinase activity b...

journal_title：Biochemical and biophysical research communications

authors： Hara K,Yonezawa K,Sakaue H,Kotani K,Kotani K,Kojima A,Waterfield MD,Kasuga M

更新日期：1995-03-17 00:00:00

abstract：:The gene encoding sericin 1 (Ser1) of silkworm (Bombyx mori) is specifically expressed in the middle silk gland cells. To identify element involved in this transcription-dependent spatial restriction, truncation of the 5' terminal from the sericin 1 (Ser1) promoter is studied in vivo. A 209bp DNA sequence upstream of ...

journal_title：Biochemical and biophysical research communications

authors： Liu Y,Yu L,Guo X,Guo T,Wang S,Lu C

更新日期：2006-03-31 00:00:00

abstract：:MicroRNAs (miRNAs) are involved in controlling hepatocyte proliferation during liver regeneration. In this study, we established the miRNAs-expression patterns of primary hepatocytes in vitro under stimulation of epidermal growth factor (EGF), and found that microRNA-21 (miR-21) was appreciably up-regulated and peaked...

journal_title：Biochemical and biophysical research communications

authors： Yan-nan B,Zhao-yan Y,Li-xi L,jiang Y,Qing-jie X,Yong Z

更新日期：2014-01-17 00:00:00

abstract：:Oxidative stress induced vascular endothelial cell injure is one of the key and initial event in the development of atherosclerosis. Septin4, as a member of GTP binding protein family, is widely expressed in the eukaryotic cells and considered to be an essential component of the cytoskeleton which is involved in many ...

journal_title：Biochemical and biophysical research communications

authors： Zhang N,Zhang Y,Zhao S,Sun Y

更新日期：2018-02-05 00:00:00

abstract：:The spectrum of resting cytochrome c oxidase is modulated by valinomycin addition, which induces a red shift of the Soret band. The enzyme is known both to fluoresce and phosphorescence, effects which can be modulated by certain protein reagents and quenchers. Valinomycin had little effect upon fluorescence at 335 nm,...

journal_title：Biochemical and biophysical research communications

authors： Butko P,He J,Nicholls P

更新日期：1992-12-30 00:00:00

abstract：:Peptides with a molecular weight of 5 kDa have been isolated from seeds of the pinto bean and red bean, respectively. The peptides manifest an N-terminal sequence with remarkable resemblance to those of cowpea 10-kDa protein precursor and garden pea disease resistance response protein. The bean peptides possess potent...

journal_title：Biochemical and biophysical research communications

authors： Ye XY,Ng TB

更新日期：2001-07-13 00:00:00

abstract：:Vasoactive intestinal peptide (VIP) is a small neuropeptide, which exerts pleiotropic functions. Based on its immunomodulatory, secretory, and possibly trophic effects, VIP is a valuable candidate molecule for the management of autoimmune disease. The purpose of this study was to develop a recombinant viral vector cap...

journal_title：Biochemical and biophysical research communications

authors： Lodde BM,Delporte C,Goldsmith CM,Tak PP,Baum BJ

更新日期：2004-06-18 00:00:00

abstract：:The effect of sex steroids on the regulation of hepatocyte resting membrane potential (Em) was investigated. In adult rat liver snips, Em was significantly lower in males than females. In prepubertal animals no sex related difference was observed and the Em was comparable to that of adult females. Exposure of the huma...

journal_title：Biochemical and biophysical research communications

authors： Motzkin SM,Feinstein J,Tiribelli C

更新日期：1990-11-15 00:00:00

abstract：:The recently detected Rieske iron-sulfur center in the membrane of the thermoacidophilic archaeon Sulfolobus acidocaldarius (Anemüller et al., 1993, FEBS Lett. 318, 61-64) was further characterized by EPR spectroscopy, coupled to redox-potentiometry and functional studies. The reduction potential is pH-dependent above...

journal_title：Biochemical and biophysical research communications

authors： Anemüller S,Schmidt CL,Schäfer G,Bill E,Trautwein AX,Teixeira M

更新日期：1994-07-15 00:00:00

abstract：:The perchloric acid-soluble protein (PSP) is an endoribonuclease and on the basis of sequence similarity has been assigned to the YjgF/YER057c/UK114 family. These family members are ubiquitous and highly conserved in evolution, and participate in regulating basic cellular metabolism. Here we present the 2.1A crystal s...

journal_title：Biochemical and biophysical research communications

authors： Zhang HM,Gao Y,Li M,Chang WR

更新日期：2010-06-18 00:00:00

abstract：:A mass screening program for congenital hypothyroidism has markedly improved prognosis of children with congenital hypothyroidism and also revealed several cases with unknown pathogenesis. We here report two independent Japanese multigeneration families with multinodular goiter (MNG) with euthyroidism and with high TS...

journal_title：Biochemical and biophysical research communications

authors： Takahashi T,Nozaki J,Komatsu M,Wada Y,Utsunomiya M,Inoue K,Takada G,Koizumi A

更新日期：2001-06-15 00:00:00

abstract：:Interleukin-15 (IL-15) is a cytokine which is highly expressed in skeletal muscle, and which stimulates muscle protein accretion in cultured skeletal muscle fibers. Using parental C2 skeletal myoblasts, no significant effects of IL-15 on skeletal muscle differentiation were observed. To test the hypothesis that IL-15 ...

journal_title：Biochemical and biophysical research communications

authors： Quinn LS,Haugk KL,Damon SE

更新日期：1997-10-09 00:00:00

abstract：:Kinetics of pinocytosis in Dictyostelium discoideum were investigated over an extended period of time (up to 6 hours) using fluorescein isothiocyanate (FITC)-dextran as a fluid-phase marker. FITC-dextran added to the medium accumulated rapidly inside the cells with a rate of influx equivalent to 9 microns3 of fluid/ce...

journal_title：Biochemical and biophysical research communications

authors： Klein G,Satre M

更新日期：1986-08-14 00:00:00

abstract：:The effects of cAMP on the expression of ETA (ET-1 selective type) receptor mRNA and on the response to endothelin-1 (ET-1) were investigated in rat aortic smooth muscle cells in primary culture, using reverse transcription polymerase chain reaction (RT-PCR) and fura-2 microfluorometry of cytosolic Ca2+ concentrations...

journal_title：Biochemical and biophysical research communications

authors： Nishimura J,Chen X,Jahan H,Shikasho T,Kobayashi S,Kanaide H

更新日期：1992-10-30 00:00:00

abstract：:Exposure to silica has been associated with progressive pulmonary inflammation and fibrosis. While the fibroblasts play an important role in the pathogenesis of silicosis, the direct interaction between silica and fibroblasts is poorly understood. We observed that silica particles stimulated intracellular ROS generati...

journal_title：Biochemical and biophysical research communications

authors： Cho YJ,Seo MS,Kim JK,Lim Y,Chae G,Ha KS,Lee KH

更新日期：1999-09-07 00:00:00

abstract：:The vitamin D pathway is related to the mass and function of skeletal muscles. Several studies have demonstrated the role of vitamin D receptor (VDR) and CYP27B1 in skeletal muscles, suggesting that these proteins may regulate skeletal muscles and their function. However, it remains unclear whether the expression of V...

journal_title：Biochemical and biophysical research communications

authors： Mori R,Yokokawa T,Fujita S

更新日期：2020-08-27 00:00:00

abstract：:Expression of the cytochrome P-450 monooxygenase activity 7-ethoxyresorufin O-deethylase (7-ERD) was surveyed in proliferating and quiescent cultures of murine cell line C-10, a non-tumorigenic line of presumed alveolar type II origin. 7-ERD activities were undetectable in subconfluent/proliferating cultures but becam...

journal_title：Biochemical and biophysical research communications

authors： Reiners JJ Jr,Pavone A,Cantu AR,Auerbach C,Malkinson AM

更新日期：1992-02-28 00:00:00