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Identification of a GTP-binding protein in the contact sites between inner and outer mitochondrial membranes.

Abstract：

:Whilst investigating whether GTP hydrolysis may be required for the import of preproteins into mitochondria we have found that a GTP-binding protein is located at the contact sites between mitochondrial inner and outer membranes. When mitochondrial outer membranes purified from rat liver were UV-irradiated in the presence of [alpha-32P]GTP, a 52 kDa protein was radiolabelled, whereas [alpha-32P]ATP did not label this protein. GTP-binding proteins were also labelled in the cytosolic and microsomal fractions, but the 52 kDa protein was concentrated in mitochondrial membranes and was the only protein specifically labelled by GTP in these membranes. Fractionation of mitochondrial membrane vesicles into outer membranes, inner membranes and contact sites between outer and inner membranes showed that the GTP-binding activity was highly enriched in contact sites, the location at which preprotein import is believed to occur. A protein of almost identical size was also found to be labelled in mitochondria from yeast.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Lithgow T,Timms M,Høj PB,Hoogenraad NJ

doi

10.1016/s0006-291x(05)81359-2

subject

Has Abstract

pub_date

1991-11-14 00:00:00

pages

1453-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)81359-2

journal_volume

180

pub_type

杂志文章

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