Abstract：
:To compare the genotoxic effects of high-LET ionizing radiation to those of low-LET radiation, we investigated the responses of human lymphoblastoid cells to DNA damage TK6 after treatment with either low-LET X rays or high-LET iron ions (1000 keV/microm). A highly localized distribution of gammaH2AX/RAD51 foci was observed in the nuclei of cells irradiated with iron ions, in sharp contrast to cells exposed to X rays, where the distribution of foci was much more uniform. This implied the occurrence of a relatively high frequency of closely spaced double-strand breaks, i.e. clustered DNA damage, after iron-ion exposure. Despite the well-established notion that clustered DNA damage is refractory to repair compared to isolated DNA lesions, there were no significant differences in the levels of clonogenic survival and apoptosis between cells treated with iron ions or X rays. Strikingly, however, cells accumulated in G(2)/M phase to a much lesser extent after iron-ion exposure than after X-ray exposure. This differential accumulation could be attributed to a much slower evacuation of the S-phase compartment in the case of cells irradiated with iron ions. Taken together, our results indicate that, relative to the situation for low-LET X rays, exposure to high-LET iron ions results in a substantially greater inhibition of S-phase progression as a result of a higher frequency of DNA replication-blocking clustered DNA damage.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Goto S,Watanabe M,Yatagai Fdoi
10.1667/0033-7587(2002)158[0678:dccpih]2.0.co;2subject
Has Abstractpub_date
2002-12-01 00:00:00pages
678-86issue
6eissn
0033-7587issn
1938-5404journal_volume
158pub_type
杂志文章abstract：:The major limitation to reaching a curative radiation dose in radioresistant tumors such as malignant gliomas is the high sensitivity to radiation and subsequent damage of the surrounding normal tissues. Novel dose delivery methods such as minibeam radiation therapy (MBRT) may help to overcome this limitation. MBRT ut...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14018.1
更新日期：2015-09-01 00:00:00
abstract：:Relative biological effectiveness (RBE) and oxygen enhancement ratio (OER) values of different neutron beams produced at the variable energy cyclotron "Cyclone" of Louvain-la-Neuve (Belgium) were determined. The neutrons were obtained by bombarding a beryllium target with 34-, 45-, 65-, or 75-MeV protons or with 50-Me...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:This report presents the results of an investigation of changes in the number of erythroid and granulocyte-macrophage colony-forming cells (GM-CFC) that had occurred in tissues of normal B6D2F1 mice 20 h after administration of a radioprotective dose (150 ng) of human recombinant interleukin-1 (rIL-1). Neutrophilia in...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:Four programs have been written to enable radiobiologists to build a computer data base of cellular dose-survival data, calculate cell survival with a correction for cell multiplicity at the time of irradiation, fit various survival models to the data by iteratively weighted least squares, and calculate the ratio of s...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:Cells from an infinite-life-span near-diploid human fibroblast cell strain, MSU-1.1, were transformed after a single exposure to 60Co gamma radiation. The frequency of transformation as measured by the number of induced foci per 10(6) cells was a linear function of dose. Cells from 13 independent foci from gamma-irrad...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:The relationship between the intracellular glutathione (GSH) concentration and the aerobic radiation response was studied in Chinese hamster ovary cells. Various degrees of GSH depletion were produced by exposure to buthionine sulfoximine (BSO) and/or diethyl maleate (DEM). Diethyl maleate did not act as a classical r...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:In a patient with Morbus Hodgkin, structural aberrations of the chromosome type in peripheral lymphocytes were analyzed during radiation therapy (accumulated target dose 44.6 Gy: 22 fractions of 1.8 Gy each and 2 fractions of 2.5 Gy each at the end of the therapy). The blood was sampled about 5 min after a fraction an...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:Radiation exposure leads to a risk for long-term deterministic and stochastic late effects. Two individuals exposed to protracted photon radiation in the radiological accident at the Lilo Military site in Georgia in 1997 received follow-up treatment and resection of several chronic radiation ulcers in the Bundeswehr H...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0774.1
更新日期：2007-05-01 00:00:00
abstract：:In boron neutron capture therapy, the absorbed dose from the (10)B(n,alpha)(7)Li reaction depends on the (10)B concentration and (10)B distribution in the irradiated volume. Thus compounds used in BNCT should have tumor-specific uptake and low accumulation in normal tissues. This study compares in a mouse model the (1...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1700.1
更新日期：2009-10-01 00:00:00
abstract：:To study the dynamics of protein recruitment to DNA lesions, ion beams can be used to generate extremely localized DNA damage within restricted regions of the nuclei. This inhomogeneous spatial distribution of lesions can be visualized indirectly and rapidly in the form of radiation-induced foci using immunocytochemic...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3374
更新日期：2005-06-01 00:00:00
abstract：:Hypoadrenocorticism, known as Addison's disease in humans, was diagnosed in six beagles after inhalation of at least 1.7 kBq/g lung of 238PuO2. Histological examination of adrenal gland specimens obtained at necropsy revealed marked adrenal cortical atrophy in all cases. Autoradiographs showed only slight alpha-partic...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Neither cell division nor growth of Saccharomyces cerevisiae were stimulated by the ultraviolet luminescence produced by adjacent exponential phase cultures of the yeast. The study included experiments in which the inocula (density = 5 X 10(7) cells cm-3) were irradiated and in which lag phase cultures (densities = 1 ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:Tissue-incorporated radionuclides impart radiation energy over extended periods of time depending on their effective half-lives. The capacity of vitamin A dissolved in soybean oil to protect against the biological effects caused by internal radionuclides is investigated. The radiochemicals examined are DNA-binding 125...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:We used Restriction Landmark Genome Scanning (RLGS) to assess, on a genome-wide basis, the mutation induction rate in mouse germ cells after radiation exposure. Analyses of 1,115 autosomal NotI DNA fragments per mouse for reduced spot intensity, indicative of loss of one copy, in 506 progeny derived from X-irradiated ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3146
更新日期：2004-04-01 00:00:00
abstract：:Based on murine survival studies, endogenous hemopoietic spleen colony formation (E-CFU), and recovery of bone marrow and splenic granulocyte-macrophage colony-forming cells (GM-CFC), it was demonstrated that the postirradiation administration of glucan, an immunomodulator and hemopoietic stimulant, enhances the radio...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The use of tritium-labeled thymidine (3H-TdR) in biological research made it necessary to develop a quick and accurate method for determination of tritium activity in tissue. After 3H-TdR incorporation into the root tip meristem of Vicia faba, total 3H activity as well as 3H-DNA activity was measured by liquid scintil...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-12-01 00:00:00
abstract：:Recent evidence has shown that translocation frequencies decline over time. This phenomenon might be explained by the co-occurrence of translocations in cells that also contain dicentrics, in which case translocations would be eliminated as a by-product of selection against dicentrics. Alternatively, a fraction of tra...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0539:tclori]2.0.co;2
更新日期：2002-05-01 00:00:00
abstract：:We report results for chromosomal aberrations in human peripheral blood lymphocytes after they were exposed to high-energy iron ions with or without shielding at the HIMAC, AGS and NSRL accelerators. Isolated lymphocytes were exposed to iron ions with energies between 200 and 5000 MeV/nucleon in the 0.1-1-Gy dose rang...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3362.1
更新日期：2005-10-01 00:00:00
abstract：:In a recent study (Int. J. Radiat. Oncol. Biol. Phys. 36, 635-639, 1996), 1H nuclear magnetic resonance (NMR) spectroscopy was used to demonstrate significant decreases in lactate levels after gamma irradiation of radiosensitive RIF-1 tumors in vitro. For comparison, we have examined the effects of gamma radiation on ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Vascular injury after radiation exposure contributes to multiple types of tissue injury through a cascade of events. Some of the earliest consequences of radiation damage include increased vascular permeability and promotion of inflammation, which is partially manifested by increased leukocyte-endothelial (L/E) intera...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14896.1
更新日期：2018-07-01 00:00:00
abstract：:The LD50 of 46 salts of metals and rare earths (lanthanoids) was determined in mice. Half the LD50 of the compounds was then combined with lethal radiation (10.5 Gy) and the modification of survival time was scored. Only the metals beryllium and indium and the rare earths cerium, lanthanum and scandium displayed activ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:The correlation between the protein content of nuclei and heat-induced cell killing was determined for HeLa cells with various levels of thermotolerance. Thermotolerance was induced by heating cells at 45 degrees C for 15 or 30 min and then incubating them at 37 degrees C for 5 or 24 h. This procedure resulted in four...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:We have used Escherichia coli B/r to test the proposal that hydroxyl radicals (.OH) are major contributors to lethal damage when bacteria in equilibrium with air or 100% nitrogen are exposed to ionizing radiation. In addition, we have tested the hypothesis that oxygen sensitizes bacterial cells to radiation by reactin...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:We investigated delayed reproductive death and chromosome bridge formation in mitotic cells in colonies of normal human embryonic cells which survived exposure to 6 Gy of X rays. Eighteen primary clones each derived from control and irradiated cultures were isolated and secondary colonies were grown up. Six of the pri...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:The efficiency of boron neutron capture therapy (BNCT) for malignant gliomas depends on the selective and absolute accumulation of (10)B atoms in tumor tissues. Only two boron compounds, BPA and BSH, currently can be used clinically. However, the detailed distributions of these compounds have not been determined. Here...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0501.1
更新日期：2007-01-01 00:00:00
abstract：:Wortmannin has been shown to be an efficient radiosensitizer. Since wortmannin is able to inhibit DNA-dependent protein kinase (DNA-PK) and double-strand break (DSB) rejoining, it is believed that its mechanism of radiation sensitization is through the inhibition of DNA-PK-mediated repair of DSBs. However, since wortm...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:We present measurements of low-energy (<18 eV) electron-stimulated desorption of anions from acetamide (CH(3)CONH(2)) and dimethyl disulfide [DMDS: (CH(3)S)(2)] films. Electron irradiation of physisorbed CH(3)CONH(2) produces H(-), CH(3)(-) and O(-) anions, whereas the H(-), CH(2)(-), CH(3)(-), S(-), SH(-) and SCH(3)(...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0023:aopsib]2.0.co;2
更新日期：2002-07-01 00:00:00
abstract：:A total of 155 primary bone sarcomas were found in 131 of the 246 beagles injected with 226Ra and 5 primary bone sarcomas were found in 4 of the 158 unexposed controls. Of these 155 bone sarcomas, 146 (94%) were osteosarcomas and 9 were non-osteosarcomas. An additional 31 primary bone sarcomas (28 osteosarcomas) devel...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:The survival of synchronous G1 or asynchronous Chinese hamster ovary cells in vitro to heat treatment may depend on the cellular population density at the time of heating and/or as the cells are cultured after heating. The addition of lethally irradiated feeder cells may increase survival at 10(-3) by as much as 10- t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:The effect of ionizing radiation (137Cs) on processing and transport of ribosomal RNA (rRNA) was studied by pulse-labeling HeLa S3 cells with [3H]uridine immediately prior to irradiation. This approach permits kinetic analysis of processing of 45 S rRNA (radiolabeled predominantly prior to irradiation) into its 28 S a...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
