Abstract：
:In a recent study (Int. J. Radiat. Oncol. Biol. Phys. 36, 635-639, 1996), 1H nuclear magnetic resonance (NMR) spectroscopy was used to demonstrate significant decreases in lactate levels after gamma irradiation of radiosensitive RIF-1 tumors in vitro. For comparison, we have examined the effects of gamma radiation on lactate levels in the more radioresistant EMT6 tumor. Single-slice (5-6 mm thick) localized 1H spectra of subcutaneous RIF-1 (untreated) and EMT6 tumors (pretreatment, 24 and 48 h postirradiation with 4, 10 or 20 Gy of gamma radiation) were measured by the selective multiple quantum coherence transfer method (Sel-MQC, approximately 4 min acquisition time). Both pretreatment lactate levels and pretreatment lactate dehydrogenase (LDH) activities were found to be similar in RIF-1 and EMT6 tumors, suggesting that steady-state lactate levels are unlikely to be reliable indices for predicting response to radiation therapy. After 10 Gy gamma irradiation, EMT6 tumors showed a 21% decrease relative to pretreatment lactate levels at 48 h (1.04 +/- 0.22 to 0.82 +/- 0.16; P = 0.06); after 20 Gy a 40% decrease was observed at 48 h (1.34 +/- 0.27 to 0.81 +/- 0.10; P = 0.07). No significant changes in lactate levels were observed in control EMT6 tumors or in tumors treated with 4 Gy of gamma radiation, in contrast to changes detected previously in RIF-1 tumors, which showed a significant decrease in lactate by 48 h for both 2 and 4 Gy. The decreased effect of radiation on lactate levels in EMT6 compared to RIF-1 tumors may be attributed to the higher hypoxic fraction and lower radiosensitivity of EMT6 tumors (Int. J. Radiat. Oncol. Biol. Phys. 10, 695-712, 1984). The decrease in lactate levels did not, however, strictly reflect the extent of the response to therapy for the high dose of 20 Gy. This study together with our earlier study (Int. J. Radiat. Oncol. Biol. Phys. 36, 635-639, 1996) provides evidence to support the hypothesis that changes in steady-state tumor lactate levels may serve as sensitive early indices of tumor response to gamma radiation at doses of the order of 2 to 4 Gy.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Aboagye EO,Bhujwalla ZM,He Q,Glickson JDsubject
Has Abstractpub_date
1998-07-01 00:00:00pages
38-42issue
1eissn
0033-7587issn
1938-5404journal_volume
150pub_type
杂志文章abstract：:After exposure to 10 or 20 J/m2 UVC light, cells of the UMN-PIE-1181 line, an embryonic cell line derived from the Indian meal moth, Plodia interpunctella, exhibited a rapid and prolonged depression in the rate of incorporation of [3H]thymidine, whereas cells of the TN-368 line, an ovarian cell line derived from Trich...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:The presence of oxygen-deficient tumor cells is a critical issue in cancer therapy. To identify tumor hypoxia, tissue partial oxygen pressure (pO2) can be measured directly. The OxyLite system allows determination of pO2 in tumors and permits continuous measurements of pO2 at a fixed point. In this study, this system ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3602.1
更新日期：2006-09-01 00:00:00
abstract：:Thymic explant cultures were used to study the radiosensitivity of nonlymphoid thymic components in dogs. Thymic fragments from fetal (50 days gestation), newborn, and juvenile (70 days old) dogs were irradiated in vitro at 0, 0.5, 1, 2, or 4 Gy prior to culture. Colonies were classified as epithelial, spindle, or mix...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:Some recent epidemiological studies have shown a positive association between cancer incidence and exposure to electromagnetic (EM) fields. Evidence from in vitro studies indicates that this effect could be due to synergistic interaction between EM fields and tumor promoters. However, no dose-response data related dir...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:Wistar rats (36) were exposed daily throughout pregnancy to a power density level of 35 mW/cm2 of 6000-MHz microwave radiation (11), sham irradiated (10), or used as control animals (15). Litters were culled to a maximum of eight F1a offspring/litter (total = 124) on Postnatal Day 1 and subjected to a series of reflex...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-02-01 00:00:00
abstract：:Wortmannin has been shown to be an efficient radiosensitizer. Since wortmannin is able to inhibit DNA-dependent protein kinase (DNA-PK) and double-strand break (DSB) rejoining, it is believed that its mechanism of radiation sensitization is through the inhibition of DNA-PK-mediated repair of DSBs. However, since wortm...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:This study investigated the biological effects and adaptive responses induced by single and repeated in vivo computed tomography (CT) scans. We postulated that, through the induction of low-level oxidative stress, repeated low-dose CT scans (20 mGy, 2 days/week, 10 weeks) could protect mice (C57BL/6) from acute effect...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2532.1
更新日期：2012-02-01 00:00:00
abstract：:We have monitored the end points of cellular survival, micronucleus formation and neoplastic transformation frequency to assess adaptation to ionizing radiation in the C3H 10T1/2 mouse embryo cell system. Plateau-phase cells were pre-exposed to an adapting dose of 0.1 to 1.5 Gy low-dose-rate gamma radiation 3.5 h prio...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Ionizing radiation induces bistranded clustered damages--two or more abasic sites, oxidized bases and strand breaks on opposite DNA strands within a few helical turns. Since clusters are refractory to repair and are potential sources of double-strand breaks (DSBs), they are potentially lethal and mutagenic. Although i...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3498.1
更新日期：2006-02-01 00:00:00
abstract：:To accurately quantify the number of single-strand breaks (SSBs) induced in plasmid DNA molecules after irradiation, a new type of assay methodology has been explored. The new method is based on the TUNEL (terminal deoxynucleotide transferase dUTP nick end-labeling) assay that was adopted for use under ELISA (enzyme-l...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1684.1
更新日期：2009-11-01 00:00:00
abstract：:To characterize the role of the cerebrovascular response in the development of brain injury after focal irradiation, 125I sources were implanted in frontal white matter of the brain of normal dogs; dose was 20 Gy, 7.5 mm from the source. Cerebral blood flow, vascular volume and mean transit time of blood were quantifi...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:Fluorescence in situ hybridization procedures were used to examine the influence of chromosome locus on the frequency and type of chromosome aberrations induced by 60Co gamma rays in the human lymphoblastoid cell line TK6. Aberrations involving the X chromosome were compared to those involving the similarly sized auto...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Tritium that is bound to organic molecules is of special risk for living systems, in particular when such molecules are components of the cell nucleus. Therefore, [3H]thymidine and [3H]arginine were studied for radiotoxicity in early mammalian embryo development. Starting with the two-cell stage, mouse embryos were in...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:While the link between risk of leukemia and acute radiation exposure is well established for large doses received acutely, uncertainty remains around the translation of these risk estimates to occupational exposure scenarios where the doses are low and accumulated over time, possibly over many years. We present leukem...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15358.1
更新日期：2019-11-01 00:00:00
abstract：:Dose assessment after radiological disasters is imperative to decrease mortality through rationally directed medical intervention. Our goal was to identify biomarkers capable of qualitative (nonirradiated/irradiated) and/or quantitative (dose) assessment of radiation exposure. Using real-time quantitative PCR, biodosi...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2419.1
更新日期：2011-07-01 00:00:00
abstract：:We studied the role of cysteine as an intracellular radiation protector under conditions in which both oxygen and thiols were monitored at 37 degrees C. In HCT-116 human colon cancer cells, the intracellular cysteine content affects the radiation survival dramatically at intermediate oxygen levels, but not at zero or ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)156[0388:tkmfro]2.0.co;2
更新日期：2001-10-01 00:00:00
abstract：:The intrinsic radiosensitivity of tumor cells is most frequently reported for asynchronous populations, although cell cycle variation in radiosensitivity is known to be significant. Linear-quadratic analyses of survival data for asynchronous human tumor cells show wide variations in the alpha coefficient with smaller ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:Rat females were exposed to a single 4.0-Gy gamma-ray dose and treated postradiation with a mixture of ATP-AET-mexamine at daily doses of 24, 12, and 3 mg/kg body wt, respectively, in drinking water throughout the period of their survival. With the radiation dose used, life shortening appeared primarily attributable t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:An analytical expression is developed for calculating the average energy deposited by an ion in a volume with dimensions less than the range of the secondary electrons produced. The expression is obtained by including two additional terms in an energy-restricted linear-energy-transfer equation. The usual energy-restri...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:In the 400-MHz 1H NMR spectra of gamma-irradiated, N2O-saturated aqueous solutions of polyuridylic acid (polyU), polycytidylic acid (polyC), polyadenylic acid (polyA), and single-stranded DNA from calf thymus, well-resolved signals of the undamaged free bases were identified. Upon incubation of the solutions at 95 deg...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:Relatively few data are available on the prevalence of hyperthyroidism (TSH concentrations of <0.3 mIU/liter, with normal or elevated concentrations of free T4) in individuals exposed to radioiodines at low levels. The accident at the Chornobyl (Chernobyl) nuclear plant in Ukraine on April 26, 1986 exposed large numbe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2003.1
更新日期：2010-12-01 00:00:00
abstract：:Female rhesus monkeys received whole-body doses of ionizing radiation in the form of single-energy protons, mixed-energy protons, X rays, and electrons. Endometriosis developed in 53% of the monkeys during a 17-year period after exposure. Incidence rates for endometriosis related to radiation type were: single-energy ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Pregnant Wistar-MS rats received a whole-body irradiation of 0-2.6 Gy gamma rays at day 20 of pregnancy. The mother rats were implanted with a diethylstilbestrol (DES) pellet 30 days after weaning, and the female pups delivered by the irradiated mother were treated with DES after maturation. Lactating rats were irradi...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:The correlation between the protein content of nuclei and heat-induced cell killing was determined for HeLa cells with various levels of thermotolerance. Thermotolerance was induced by heating cells at 45 degrees C for 15 or 30 min and then incubating them at 37 degrees C for 5 or 24 h. This procedure resulted in four...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:Dose-response relationships observed in laboratory animals can be used to identify possible human risk factors and may also be used in a quantitative manner when human data are not available. This paper presents an analysis of the dose dependency of osteosarcoma incidence in beagle dogs given a single inhalation expos...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:Exposure to ionizing radiation or a variety of chemical agents is known to increase the risk of developing malignancy and many tumors have been linked to inflammatory processes. In most studies, the potentially harmful effects of ionizing radiation or other agents are considered in isolation, mainly due to the large n...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2690.1
更新日期：2012-05-01 00:00:00
abstract：:Both plant senescence and plant response to ionizing radiation involve changes in gene expression and epigenetic profiles, that rely on the formation of reactive oxygen species. However, how the developmental stage of a plant affects its response to ionizing radiation has not been extensively studied. In this study, o...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13840.1
更新日期：2015-02-01 00:00:00
abstract：:Using five transplantable murine tumors (SCC-VII, B16F1, KHT-C, KHT-LP1, RIF-1), measurements of tumor hypoxia have been made with two techniques which have the potential to be used for assessing oxygenation in human tumors (the Eppendorf pO2 Histograph and binding of [3H]misonidazole) and have been compared with an e...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:After total-body exposure to various doses of ionizing radiation, the ability of lymphocytes to interact specifically with high endothelial venules of rat cervical and mesenteric lymph nodes was analyzed in frozen sections. Following a radiation dose of 1.5 Gy, high endothelial venules remained intact and the binding ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:An understanding of proton radiobiology is critical for optimization of both proton radiotherapy and assessment of carcinogenesis risk from space radiation. Although the physical aspects of proton beam radiobiology is well understood, the biological aspects, particularly the complex biological end points, have been un...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/RR2839.1
更新日期：2013-03-01 00:00:00
