Abstract：
:We used Restriction Landmark Genome Scanning (RLGS) to assess, on a genome-wide basis, the mutation induction rate in mouse germ cells after radiation exposure. Analyses of 1,115 autosomal NotI DNA fragments per mouse for reduced spot intensity, indicative of loss of one copy, in 506 progeny derived from X-irradiated spermatogonia (190, 237 and 79 mice in 0-, 3-, and 5-Gy groups, respectively), permitted us to identify 16 mutations affecting 23 fragments in 20 mice. The 16 mutations were composed of eight small changes (1-9 bp) at microsatellite sequences, five large deletions (more than 25 kb), and three insertions of SINE B2 or LINE1 transposable elements. The maximum induction rate of deletion mutations was estimated as (0.17 +/- 0.09) x 10(-5)/locus Gy(-1). The estimate is considerably lower than 1 x 10(-5)/locus Gy(-1), the mean induction rate of deletion mutations at Russell's 7 loci, which assumed that deletion mutations comprise 50% of all mutations. We interpret the results as indicating that the mean induction rate of mutations in the whole genome may be substantially lower than that at the 7 loci. We also demonstrate the applicability of RLGS for detection of human mutations, which allows direct comparisons between the two species.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Asakawa J,Kuick R,Kodaira M,Nakamura N,Katayama H,Pierce D,Funamoto S,Preston D,Satoh C,Neel JV,Hanash Sdoi
10.1667/rr3146subject
Has Abstractpub_date
2004-04-01 00:00:00pages
380-90issue
4eissn
0033-7587issn
1938-5404pii
RR3146journal_volume
161pub_type
杂志文章abstract：:The effect of age at exposure on induction of mammary carcinoma was studied in female rats of the inbred WAG/Rij strain that were treated with estrogen. Groups of 40 animals were exposed to a single total-body dose of 1 or 2 Gy of 137Cs gamma radiation at age 8, 10, 12, 15, 22, 36 or 64 weeks. Hormone levels in the an...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:In the event of a radiation accident or attack, it will be imperative to quickly assess the amount of radiation exposure to accurately triage victims for appropriate care. RNA-based radiation dosimetry assays offer the potential to rapidly screen thousands of individuals in an efficient and cost-effective manner. Howe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr1977.1
更新日期：2011-02-01 00:00:00
abstract：:Cytoglobin is a recently identified vertebrate globin whose functions include scavenging reactive oxygen and nitrosative species. In tumor cells, CYGB may function as a tumor suppressor gene. Here we show that knockdown of cytoglobin expression can sensitize human glioma cells to oxidative stress induced by chemical i...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2517.1
更新日期：2011-08-01 00:00:00
abstract：:Sequential examinations were done on the pulmonary cytokinetics and pulmonary lesions in rats after inhalation exposure to (239)PuO(2) aerosols to investigate the pathogenesis of lung tumors. Total cell yields of lavaged bronchoalveolar cells as well as the estimated numbers of pulmonary alveolar macrophages were sign...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)154[0253:ppolti]2.0.co;2
更新日期：2000-09-01 00:00:00
abstract：:Therapeutic gain factors (TGFs) have been determined for three spontaneous tumors of the C3H mouse treated by photons + normobaric oxygen (O2(1) ATA), photons + hyperbaric oxygen (O2 3 ATA), photons + misonidazole, or fast neutrons. The tumors were early generation isotransplants of spontaneous tumors: MCaIV, a mammar...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:It is well known that ionizing radiation-induced toxicity to normal tissue has functional consequences in the brain. However, the underlying molecular alterations have yet to be elucidated. We have previously reported cognitive impairments with concomitant changes in dendritic complexity, spine density and inflammatio...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15061.1
更新日期：2018-11-01 00:00:00
abstract：:In the event of a large-scale radiation exposure, accurate and quick assessment of radiation dose received would be critical for triage and medical treatment of large numbers of potentially exposed individuals. Current methods of biodosimetry, such as the dicentric chromosome assay, are time consuming and require soph...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14655.1
更新日期：2017-06-01 00:00:00
abstract：:Chinese hamster V79 cells show a complex X-ray survival response which is characterized by hypersensitivity followed by increased resistance as the dose increases to 1 Gy. This hypersensitivity can be eliminated by pretreating cells with X rays or hydrogen peroxide. Accordingly, the protective effect that results from...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:In this study, we investigated the effect of (131)I gelatin microspheres ((131)I-GMSs) on human hepatocellular carcinoma cells (HepG2) in nude mice (Balb/c) and the biodistribution of (131)I-GMSs after intratumoral injection. The treatment group and control group animals received intratumoral injections of 1 mCi (131)...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13539.1
更新日期：2014-04-01 00:00:00
abstract：:Some recent epidemiological studies have shown a positive association between cancer incidence and exposure to electromagnetic (EM) fields. Evidence from in vitro studies indicates that this effect could be due to synergistic interaction between EM fields and tumor promoters. However, no dose-response data related dir...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:The relationship between apoptosis induced by gamma radiation and glutathione in cells of two human cancer cell lines, HeLa from cervix carcinoma and MCF-7 from mammary carcinoma, was examined. MCF-7 cells appeared to be more radioresistant than HeLa cells, and radiation-induced apoptosis, which was monitored by asses...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0578.1
更新日期：2007-03-01 00:00:00
abstract：:Radon is recognized as a public health concern for indoor exposure. Precise quantification derived from occupational exposure in miners is still needed for estimating the risk and the factors that modify the dependence on cumulated exposure. The present paper reports on relationship between radon exposure and lung can...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0848.1
更新日期：2008-02-01 00:00:00
abstract：:Irradiation of human melanoma (MeWo, Be11) and squamous cell carcinoma (4451, 4197) cells induces cell cycle blocks from which the cells recover to re-enter mitosis after 40-60 h. In the TP53 mutant cell lines, MeWo and 4451, irradiation induces a G(2)-phase block, where the fraction of cells in G(2) phase reaches a m...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)154[0640:aogmpb]2.0.co;2
更新日期：2000-12-01 00:00:00
abstract：:Angiogenesis defines the many steps involved in the growth and migration of endothelial cell-derived blood vessels. This process is necessary for the growth and metastasis of tumors, and considerable effort is being expended to find inhibitors of tumor angiogenesis. This usually involves screening of potential anti-an...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0171:rdaion]2.0.co;2
更新日期：2001-01-01 00:00:00
abstract：:Currently available treatments for radiation-induced nausea and vomiting either are ineffective or reduce performance. The new antiemetic and gastrokinetic agent zacopride was tested in rhesus monkeys to assess its behavioral toxicity and its ability to inhibit radiation-induced emesis. Zacopride (intragastric, 0.3 mg...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:The threat of exposure to ionizing radiation from a nuclear reactor accident or deliberate terrorist actions is a significant public health concern. The lung is particularly susceptible to radiation-induced injury from external sources or inhalation of radioactive particles from radioactive fallout. Radiation-induced ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14686.1
更新日期：2017-11-01 00:00:00
abstract：:Although tissue mast cells are derived from the bone marrow, some descendants of bone marrow-derived precursors retain the ability to proliferate and differentiate into mast cells even after localization in the skin. The purpose of the present study was to determine the D0 values for mast-cell precursors in the bone m...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:The response of the testes of two strains of adult rats (Sprague-Dawley and Wistar) to graded single doses and split doses of 230 kVp X rays has been investigated. A marked difference was noted between the strains in the response of the clonogenic spermatogonia to irradiation, as measured histologically by the repopul...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:We have assessed the effects of two radiomodifying conditions, glutathione (GSH) depletion and hypoxia, on the formation and repair of radiation-induced chromatin damage, specifically DNA-protein cross-links (DPC). As measured by a nitrocellulose filter-binding assay, untreated V79 cells contain a low level of DPC (1-...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:Aberrant expression of microRNAs (miRNAs) is frequently associated with a variety of cancers, including breast cancer. We and others have demonstrated that radiation-induced rat mammary cancer exhibits a characteristic gene expression profile and a random increase in aberrant DNA copy number; however, the role of aber...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2927.1
更新日期：2013-02-01 00:00:00
abstract：:Alteration of adhesion molecule expression on endothelial cells has a direct connection with ionizing radiation-induced atherosclerosis, which is an adverse effect observed after radiotherapy. However, minimal attention has been given to monocytes/macrophages role in atherosclerosis development, which are exposed to t...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3119.1
更新日期：2013-01-01 00:00:00
abstract：:New data are given on the use of oxalic acid, in light and heavy water solutions, for in-pile chemical dosimetry. The method of calculating the absorbed doses in low-Z materials is described. Results of chemical measurements in the core of the heavy-water-moderated reactor at Vinca (Yugoslavia) are compared with the r...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-12-01 00:00:00
abstract：:Radiation-induced heart disease (RIHD) is a serious side effect of radiotherapy for intrathoracic and chest wall tumors. The threshold dose for development of clinically significant RIHD is believed to be lower than previously assumed. Therefore, research into mechanisms of RIHD has gained substantial momentum. RIHD b...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/rr2933.1
更新日期：2012-07-01 00:00:00
abstract：:Mortality data for B6CF1 mice exposed to 60Co gamma rays for the duration of life were used to make quantitative predictions of age-specific mortality observed in comparably exposed beagles. Simple Kaplan-Meier survival curves for the beagles and their 95% confidence intervals were computed for each dose-rate group ob...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:Three experiments failed to provide consistent evidence for an effect of 2.70 GHz pulsed microwave radiation up to 20 mW/cm2 on pentylenetetrazol-induced seizures or on the efficacy of chlordiazepoxide for counteracting such seizures. Microwave radiation counteracted the hypothermic effects of chlordiazepoxide without...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-12-01 00:00:00
abstract：:We have investigated how radiation quality affects the induction of chromosomal aberrations in human cells. Human lymphocytes were irradiated in vitro with various energies of accelerated high charge and energy (HZE) particles including oxygen, neon, silicon, titanium and iron. Chromosome damage was assessed using thr...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3291.1
更新日期：2013-07-01 00:00:00
abstract：:The well-established process of potentially lethal damage (PLD) repair enhances plateau-phase cell survival after exposure to ionizing radiation. PLD repair requires that confluent cells be incubated prior to plating for a colony-forming assay rather than being plated immediately. Enhanced double-strand break (DSB) re...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr1423.1
更新日期：2008-10-01 00:00:00
abstract：:Radiation-induced complications in bone and cartilage are of increasing concern due to potential long-term effects in cancer survivors. Healthy articular cartilage may be exposed to radiation during either chondrosarcoma treatment or in-field radiotherapy of tumors located in close proximity to articulation. Cartilage...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/RR13928.1
更新日期：2015-02-01 00:00:00
abstract：:Previous work showed that treatment of plateau-phase Chinese hamster ovary cells with the radiomimetic double-strand cleaving agent bleomycin induced very small deletions as well as interchromosomal reciprocal translocations, both of which could be ascribed to errors in end joining of DNA double-strand breaks. In an a...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0327:bstsbd]2.0.co;2
更新日期：2002-09-01 00:00:00
abstract：:Astronauts traveling outside Earth's magnetosphere risk exposure to charged particle radiation that may cause neurophysiological changes and behavioral deficits. Although proton particles comprise a large portion of the space radiation environment, little has been published on the effects of low-dose proton radiation ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr13903.1
更新日期：2015-08-01 00:00:00
