RNA damage and inhibition of neoplastic endothelial cell growth: effects of human and amphibian ribonucleases.

Abstract:

:Angiogenesis defines the many steps involved in the growth and migration of endothelial cell-derived blood vessels. This process is necessary for the growth and metastasis of tumors, and considerable effort is being expended to find inhibitors of tumor angiogenesis. This usually involves screening of potential anti-angiogenic compounds on endothelial cells. To this end, two candidate anti-angiogenic RNA-damaging agents, onconase and (-4)rhEDN, were screened for their effects on endothelial cell proliferation using three distinct types of endothelial cells in culture: HPV-16 E6/E7-immortalized human umbilical vein endothelial cells (HUVECs), a Kras-transformed HPV-16 E6/E7 HUVEC (Rhim et al., Carcinogenesis 4, 673-681, 1998), and primary HUVECs. Onconase similarly inhibited proliferation in all three cell lines (IC(50) = 0.3-1.0 microM) while (-4)rhEDN was more effective on immortalized HUVEC cell lines (IC(50) = 0.02-0.06 microM) than on primary HUVECs (IC(50) > 0.1 microM). Differential sensitivity to these agents implies that more than one endothelial cell type must be used in proliferation assays to screen for novel anti-angiogenic compounds.

journal_name

Radiat Res

journal_title

Radiation research

authors

Newton DL,Kaur G,Rhim JS,Sausville EA,Rybak SM

doi

10.1667/0033-7587(2001)155[0171:rdaion]2.0.co;2

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

171-174

issue

1 Pt 2

eissn

0033-7587

issn

1938-5404

journal_volume

155

pub_type

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