Abstract:
:Angiogenesis defines the many steps involved in the growth and migration of endothelial cell-derived blood vessels. This process is necessary for the growth and metastasis of tumors, and considerable effort is being expended to find inhibitors of tumor angiogenesis. This usually involves screening of potential anti-angiogenic compounds on endothelial cells. To this end, two candidate anti-angiogenic RNA-damaging agents, onconase and (-4)rhEDN, were screened for their effects on endothelial cell proliferation using three distinct types of endothelial cells in culture: HPV-16 E6/E7-immortalized human umbilical vein endothelial cells (HUVECs), a Kras-transformed HPV-16 E6/E7 HUVEC (Rhim et al., Carcinogenesis 4, 673-681, 1998), and primary HUVECs. Onconase similarly inhibited proliferation in all three cell lines (IC(50) = 0.3-1.0 microM) while (-4)rhEDN was more effective on immortalized HUVEC cell lines (IC(50) = 0.02-0.06 microM) than on primary HUVECs (IC(50) > 0.1 microM). Differential sensitivity to these agents implies that more than one endothelial cell type must be used in proliferation assays to screen for novel anti-angiogenic compounds.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Newton DL,Kaur G,Rhim JS,Sausville EA,Rybak SMdoi
10.1667/0033-7587(2001)155[0171:rdaion]2.0.co;2subject
Has Abstractpub_date
2001-01-01 00:00:00pages
171-174issue
1 Pt 2eissn
0033-7587issn
1938-5404journal_volume
155pub_type
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journal_title:Radiation research
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journal_title:Radiation research
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)154[0375:ivaive]2.0.co;2
更新日期:2000-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
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doi:
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doi:
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
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