Abstract：
:Human T lymphocytes can be used to determine the frequency and molecular spectrum of somatic cell gene mutations induced by ionizing radiations both in vivo and in vitro. In vitro exposure of these G0 cells to low-LET 137Cs gamma rays results in the induction of HPRT mutations and a predominant molecular spectrum of DNA deletions and rearrangements, particularly total gene deletions (11-12%). Similar results are found in samples from humans exposed to low-LET radiation from 131I. The doubling dose for mutation induction is calculated to be 0.8 and 1.0 Gy from these exposures performed in vitro and in vivo, respectively. In vitro studies of the effects of high-LET radiation from exposure to 222Rn also showed an induction of HPRT mutations, with a doubling dose of approximately 0.2 Gy. With this radiation, the predominant mutations were small partial deletions, with less than 2% total gene deletions. Studies of humans exposed to high-LET radiation from 239Pu showed an increased HPRT mutant frequency for the group, although no significant dosimetry could be defined. In contrast to the humans exposed to 131I, no increase in the frequency of total gene deletions was found. This is consistent with the results for 222Rn in vitro. The available data show that radiation quality affects both the efficiency of induction and the molecular spectrum of HPRT mutations in human T lymphocytes both in vitro and in vivo. The mutational spectrum may be relatively specific for radiations of different quality and thus allow a more precise measurement of the induction of somatic gene mutations resulting from individual exposures to radiation, and thereby provide more sensitive assessments of health risks.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Albertini RJ,Clark LS,Nicklas JA,O'Neill JP,Hui TE,Jostes Rsubject
Has Abstractpub_date
1997-11-01 00:00:00pages
S76-86issue
5 Suppleissn
0033-7587issn
1938-5404journal_volume
148pub_type
杂志文章abstract：:Abstract Pataky, K., Villanueva, G., Liani, A., Zgheib, O., Jenkins, N., Halazonetis, D. J., Halazonetis, T. D. and Brugger, J. Microcollimator for Micrometer-Wide Stripe Irradiation of Cells Using 20-30 keV X Rays. Radiat. Res. 172, 252-259 (2009). The exposure of subnuclear compartments of cells to ionizing radiatio...
journal_title：Radiation research
pub_type： 杂志文章
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更新日期：2009-08-01 00:00:00
abstract：:A novel technique has been employed to investigate the simultaneous damage to DNA components induced by soft X rays (1.5 keV) and low-energy electrons (0-30 eV) in thin films of thymidine deposited on glass and tantalum substrates and irradiated under atmospheric pressure and temperature. The films were surrounded by ...
journal_title：Radiation research
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更新日期：2014-06-01 00:00:00
abstract：:We have used Escherichia coli B/r to test the proposal that hydroxyl radicals (.OH) are major contributors to lethal damage when bacteria in equilibrium with air or 100% nitrogen are exposed to ionizing radiation. In addition, we have tested the hypothesis that oxygen sensitizes bacterial cells to radiation by reactin...
journal_title：Radiation research
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更新日期：1987-02-01 00:00:00
abstract：:Some cells have been reported to show greater resistance to drugs or radiation when growing with close intercellular contacts in spheroids or in solid tumors than when growing with few intercellular contacts in sparse cultures. In some cases this increased resistance reflects an increased capacity of cells in close co...
journal_title：Radiation research
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doi：
更新日期：1986-09-01 00:00:00
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abstract：:Technological developments in radiation therapy and other cancer therapies have led to a progressive increase in five-year survival rates over the last few decades. Although acute effects have been largely minimized by both technical advances and medical interventions, late effects remain a concern. Indeed, the need t...
journal_title：Radiation research
pub_type： 杂志文章,评审
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更新日期：2012-04-01 00:00:00
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journal_title：Radiation research
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doi：
更新日期：1993-11-01 00:00:00
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doi：10.1667/RR14018.1
更新日期：2015-09-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0857.1
更新日期：2007-07-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15283.1
更新日期：2019-05-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)153[0587:aeowbp]2.0.co;2
更新日期：2000-05-01 00:00:00
abstract：:Relative biological effectiveness (RBE) and oxygen enhancement ratio (OER) values of different neutron beams produced at the variable energy cyclotron "Cyclone" of Louvain-la-Neuve (Belgium) were determined. The neutrons were obtained by bombarding a beryllium target with 34-, 45-, 65-, or 75-MeV protons or with 50-Me...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:Ionizing radiation can induce chromosome instability that is transmitted over many generations after irradiation in the progeny of surviving cells, but it remains unclear why this instability can be transmitted to the progeny. To acquire knowledge about the transmissible nature of genomic instability, we transferred a...
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pub_type： 杂志文章
doi：10.1667/RR0835.1
更新日期：2007-06-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
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更新日期：2001-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1667/RR15130.1
更新日期：2019-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2001-06-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
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更新日期：2013-05-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
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doi：
更新日期：1994-04-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
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更新日期：2013-09-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0110:aowilc]2.0.co;2
更新日期：2002-07-01 00:00:00
abstract：:We report here on a qualitative and quantitative comparison of four kinetic photon cell survival models. The commonly used linear-quadratic model extended by a dose protraction factor, the lethal potentially lethal model, the repair misrepair model and the recently reported Giant LOop Binary LEsion (GLOBLE) model are ...
journal_title：Radiation research
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)154[0375:ivaive]2.0.co;2
更新日期：2000-10-01 00:00:00
abstract：:The microdosimetry of (10)B thermal neutron capture reactions should be considered as an essential step to be followed before studying the radiobiological aspects of boron neutron capture therapy. The boron dose itself is insufficient as the only quantity used to describe the biological effectiveness of the (10)B reac...
journal_title：Radiation research
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doi：10.1667/rr3257
更新日期：2004-12-01 00:00:00
