Abstract:
:Proton exposure induces mutations and cancer, which are presumably linked. Because protons are abundant in the space environment and significant uncertainties exist for the effects of space travel on human health, the purpose of this study was to identify the types of mutations induced by exposure of mammalian cells to 4-5 Gy of 1 GeV protons. We used an assay that selects for mutations affecting the chromosome 8-encoded Aprt locus in mouse kidney cells and selected mutants after proton exposure both in vivo and in cell culture. A loss of heterozygosity (LOH) assay for DNA preparations from the in vivo-derived kidney mutants revealed that protons readily induced large mutational events. Fluorescent in situ hybridization painting for chromosome 8 showed that >70% of proton-induced LOH patterns resembling mitotic recombination were in fact the result of nonreciprocal chromosome translocations, thereby demonstrating an important role for DNA double-strand breaks in proton mutagenesis. Large interstitial deletions, which also require the formation and resolution of double-strand breaks, were significantly induced in the cell culture environment (14% of all mutants), but to a lesser extend in vivo (2% of all mutants) suggesting that the resolution of proton-induced double-strand breaks can differ between the intact tissue and cell culture microenvironments. In total, the results demonstrate that double-strand break formation is a primary determinant for proton mutagenesis in epithelial cell types and suggest that resultant LOH for significant genomic regions play a critical role in proton-induced cancers.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Turker MS,Grygoryev D,Dan C,Eckelmann B,Lasarev M,Gauny S,Kwoh E,Kronenberg Adoi
10.1667/RR3174.1subject
Has Abstractpub_date
2013-05-01 00:00:00pages
521-9issue
5eissn
0033-7587issn
1938-5404journal_volume
179pub_type
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journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:
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doi:
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journal_title:Radiation research
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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