Abstract：
:Bcl-X(S) is a pro-apoptosis member of the Bcl2 family that has been shown to induce cell death and enhance chemosensitivity. We have investigated the effect of Bcl-X(S) overexpression on radiation sensitivity. Using a tetracycline-repressible system, we found that removal of tetracycline for 16 h induced Bcl-X(S) and reduced the surviving fraction of NIH 3T3 cells to 25%. However, radiation sensitivity was not significantly affected by Bcl-X(S) expression; the mean inactivation doses for Bcl-X(S) repressed and Bcl-X(S) induced cells were 2.7 +/- 0.3 and 2.3 +/- 0.1 Gy, respectively. We conclude that Bcl-X(S) induces cell death without affecting radiation sensitivity. These results suggest that mitochondrial pathways to apoptosis may not have a significant role in survival after irradiation.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Morgan MA,Poynter JN,Maybaum J,Lawrence TSdoi
10.1667/rr3169subject
Has Abstractpub_date
2004-05-01 00:00:00pages
535-9issue
5eissn
0033-7587issn
1938-5404pii
RR3169journal_volume
161pub_type
杂志文章abstract：:In a scenario involving a nuclear detonation during war or a terrorist attack, acute radiation exposure combined with thermal and blast effects results in severe skin injury. Although the cutaneous injury in such a scenario may not be lethal, it may lead to inflammation, delayed wound healing and loss of the skin barr...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14884.1
更新日期：2018-04-01 00:00:00
abstract：:Human immortalized keratinocytes were exposed to a range of single or fractionated doses of gamma rays from (60)Co, to medium harvested from donor cells exposed to these protocols, or to a combination of radiation and irradiated cell conditioned medium (ICCM). The surviving fractions after direct irradiation or exposu...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0626:badeaf]2.0.co;2
更新日期：2002-11-01 00:00:00
abstract：:The hearts of mature male rats of the Wistar and Sprague-Dawley strains were locally irradiated with single doses of 17.5 and 20.0 Gy of X rays, respectively. These two dose levels had previously been shown to result in a comparable latent period between irradiation and the death of rats of these two strains from card...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Puskin, J. S. What Can Epidemiology Tell Us about Risks at Low Doses? Radiat. Res. 169, 122-124 (2008). Limitations on statistical power preclude direct detection and quantification of radiogenic cancer risks at very low (environmental) levels of low-LET radiation through epidemiological studies. Given this limitation...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1187.1
更新日期：2008-01-01 00:00:00
abstract：:Previous work from our laboratory suggested that neuronal chromatin structure may be altered immediately after exposure to ionizing radiation. In the present study, whole brains of 4-month-old male Fisher 344 rats were irradiated with a dose of 25 Gy. The kinetics of restoring the chromatin structure to its unirradiat...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:The use of tritium-labeled thymidine (3H-TdR) in biological research made it necessary to develop a quick and accurate method for determination of tritium activity in tissue. After 3H-TdR incorporation into the root tip meristem of Vicia faba, total 3H activity as well as 3H-DNA activity was measured by liquid scintil...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-12-01 00:00:00
abstract：:Characteristic of damage introduced in DNA by ionizing radiation is the induction of a wide range of lesions. Single-strand breaks (SSBs) and base damages outnumber double-strand breaks (DSBs). If unrepaired, these lesions can lead to DSBs and increased mutagenesis. XRCC1 and DNA polymerase beta (polbeta) are thought ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0750.1
更新日期：2007-09-01 00:00:00
abstract：:Effects of 250-kV X rays on epidermal Langerhans cells were studied in CBA/CaH mice. One group received 20 Gy to the feet, another 8 Gy to the whole body, and a third both the 8 Gy whole-body and a 12 Gy local dose to the feet. Mice from each group and controls were sacrificed at intervals from 1 to 64 days later. ATP...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:Potentially lethal damage (PLD) repair has been defined as that property conferring the ability of cells to recover from DNA damage depending on the postirradiation environment. Using a novel cyclin dependent kinase 1 inhibitor RO-3306 to arrest cells in the G2 phase of the cell cycle, examined PLD repair in G2 in cul...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13744.1
更新日期：2014-10-01 00:00:00
abstract：:Chinese hamster V79 cells grown for several hours in suspension culture form spheroids which are more resistant to killing by ionizing radiation than cells grown on petri dishes, a phenomenon known as the "contact effect." Previous results using the alkali-unwinding assay as a measure of DNA damage have implicated dif...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Oncogenic transformation of C3H 10T1/2 cells was determined after exposure to graded doses of 4.3-MeV alpha particles LET = 101 keV/microns. The source of alpha particles was 244Cm and the irradiation was done in an irradiation chamber built for the purpose. Graded doses in the range of 0.2 to 300 cGy were studied wit...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:Human keratinocytes show a bystander effect when exposed to low doses of low-LET radiation. In this paper, data are presented showing a method of correcting the overall survival curve to enable analysis of the relative contributions of the bystander effect and the effect attributable to direct interaction of the radia...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)153[0508:rcobat]2.0.co;2
更新日期：2000-05-01 00:00:00
abstract：:Ionizing radiations induce mutations which can be detected both in coding sequences (Hprt locus) by measuring the frequency of 6-thioguanine-resistant cells and in minisatellite sequences by DNA fingerprint analysis. We analyzed the effects of irradiation with low-energy protons (31 keV/pm) and, for comparison, with u...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:We investigated delayed reproductive death and chromosome bridge formation in mitotic cells in colonies of normal human embryonic cells which survived exposure to 6 Gy of X rays. Eighteen primary clones each derived from control and irradiated cultures were isolated and secondary colonies were grown up. Six of the pri...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:Long-term retention of 133Ba in the trachea from intratracheally administered BaSO4 particles was determined by both serial sacrifice and external scanning methods up to 6 months after injection. The amount of 133Ba retained 1 week after injection in the caudal region of the trachea, where the tip of the cannula had b...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:Identification of medical countermeasures (MCM) to mitigate radiation damage and/or protect first responders is a compelling unmet medical need. The prostaglandin E2 (PGE2) analog, 16,16 dimethyl-PGE2 (dmPGE2), has shown efficacy as a radioprotectant and radiomitigator that can enhance hematopoiesis and ameliorate int...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RADE-20-00181.1
更新日期：2020-12-10 00:00:00
abstract：:Based on murine survival studies, endogenous hemopoietic spleen colony formation (E-CFU), and recovery of bone marrow and splenic granulocyte-macrophage colony-forming cells (GM-CFC), it was demonstrated that the postirradiation administration of glucan, an immunomodulator and hemopoietic stimulant, enhances the radio...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Phosphorylated ATM immunofluorescence staining was used to investigate the dose-response relationship for the number of DNA double-strand breaks (DSBs) induced in primary normal human fibroblasts irradiated with doses from 1.2 to 200 mGy. The induction of DSBs showed a supralinear dose-response relationship. Radiation...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1255.1
更新日期：2008-09-01 00:00:00
abstract：:In an effort to identify proteomic changes that may be useful for radiation biodosimetry, human cells of hematological origin were treated with ionizing radiation or mock-irradiated and then harvested at different times after treatment. Protein lysates were generated from these cells and evaluated by Western blotting ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1638.1
更新日期：2009-05-01 00:00:00
abstract：:X rays have been shown to induce two forms of potentially lethal damage (PLD), "fast-repairing" PLD and "slowly repairing" PLD, whose repair is completed in 1 h and 4-6 h, respectively. In this study three modes of treatment with hypertonic solutions containing different NaCl concentrations for different durations (0....
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:A new model for the survival of bacteria exposed to ionizing radiation is constructed in the framework of a target theory based on microdosimetric concepts, where single- and double-strand breaks of DNA and their repair in vivo can be described consistently in terms of the microdosimetric quantity j (number of effecti...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Mycoplasma infection of mammalian cells in culture is a common occurrence that can affect the results of experimental protocols. Current methods of eliminating mycoplasma from cell cultures are usually tedious, time-consuming, and sometimes unsuccessful. In the present study, four cultured brain tumor cell lines (huma...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:The Radiological Research Accelerator Facility (RARAF) is in its 50th year of operation. It was commissioned on April 1, 1967 as a collaboration between the Radiological Research Laboratory (RRL) of Columbia University, and members of the Medical Research Center of Brookhaven National Laboratory (BNL). It was initiall...
journal_title：Radiation research
pub_type： 历史文章,杂志文章
doi：10.1667/RR002CC.1
更新日期：2017-04-01 00:00:00
abstract：:To date, there are no safe and effective drugs available for protection against ionizing radiation damage. Therefore, a great need exists to identify and develop non-toxic agents that will be useful as radioprotectors or postirradiation therapies under a variety of operational scenarios. We have developed a new pharma...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2657.1
更新日期：2012-05-01 00:00:00
abstract：:We collected initial quantitative information on the effects of high-dose carbon (12C) ions compared to photons on vascular damage in anaplastic rat prostate tumors, with the goal of elucidating differences in response to high-LET radiation, using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Syngene...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15459.1
更新日期：2020-01-01 00:00:00
abstract：:Therapeutic irradiation of the brain can cause a progressive cognitive dysfunction that may involve defects in neurogenesis. In an effort to understand the mechanisms underlying radiation-induced stem cell dysfunction, neural precursor cells isolated from the adult rat hippocampus were analyzed for acute (0-24 h) and ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3112
更新日期：2004-01-01 00:00:00
abstract：:We have assessed the effects of two radiomodifying conditions, glutathione (GSH) depletion and hypoxia, on the formation and repair of radiation-induced chromatin damage, specifically DNA-protein cross-links (DPC). As measured by a nitrocellulose filter-binding assay, untreated V79 cells contain a low level of DPC (1-...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:The adaptive response to ionizing radiation may be mediated by the induction of antioxidant defense mechanisms, accelerated repair or altered cell cycle progression after the conditioning dose. To gain new insight into the mechanism of the adaptive response, nondividing lymphocytes and fibroblasts were used to elimina...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3430.1
更新日期：2005-10-01 00:00:00
abstract：:This is the 14th report in a series of periodic general reports on mortality in the Life Span Study (LSS) cohort of atomic bomb survivors followed by the Radiation Effects Research Foundation to investigate the late health effects of the radiation from the atomic bombs. During the period 1950-2003, 58% of the 86,611 L...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2629.1
更新日期：2012-03-01 00:00:00
abstract：:Polonium-210 is a naturally occurring radioactive element that decays by emitting an alpha particle. It is in the air we breathe and also a component of tobacco smoke. Polonium-210 is used as an anti-static device in printing presses and gained widespread notoriety in 2006 after the poisoning and subsequent death of a...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13395.1
更新日期：2014-02-01 00:00:00
