Flow Cytometry Definition of Rat Mammary Epithelial Cell Populations and Their Distinct Radiation Responses.

abstract：:The present study was undertaken to standardize a dichlorofluorescein (DCF) assay for measurement of radiation-induced oxidation of dichlorofluorescin (DCFH) substrate in MCF-10 cells. This assay was highly sensitive and capable of detecting increased DCFH oxidation in the cells exposed to gamma radiation at doses as ...

journal_title：Radiation research

authors： Wan XS,Zhou Z,Ware JH,Kennedy AR

更新日期：2005-02-01 00:00:00

abstract：:To understand the biophysical mechanism(s) underlying the induction of cell death by the decay of the Auger electron emitter iodine-125 in DNA, Chinese hamster V79 lung fibroblasts were labeled with 5-[(125)I]iodo-2'-deoxyuridine ((125)IdU) for two doubling times and frozen and stored at -135 degrees C in the presence...

journal_title：Radiation research

authors： Walicka MA,Ding Y,Adelstein SJ,Kassiss AI

更新日期：2000-09-01 00:00:00

abstract：:Ionizing radiation can induce chromosome instability that is transmitted over many generations after irradiation in the progeny of surviving cells, but it remains unclear why this instability can be transmitted to the progeny. To acquire knowledge about the transmissible nature of genomic instability, we transferred a...

journal_title：Radiation research

authors： Mukaida N,Kodama S,Suzuki K,Oshimura M,Watanabe M

更新日期：2007-06-01 00:00:00

abstract：:The survival of synchronous G1 or asynchronous Chinese hamster ovary cells in vitro to heat treatment may depend on the cellular population density at the time of heating and/or as the cells are cultured after heating. The addition of lethally irradiated feeder cells may increase survival at 10(-3) by as much as 10- t...

journal_title：Radiation research

authors： Highfield DP,Holahan EV,Holahan PK,Dewey WC

更新日期：1984-01-01 00:00:00

abstract：:To elucidate the nature and kinetics of DNA strand breaks caused by low-energy Auger electron emitters, we compared the yields of DNA breaks in supercoiled pUC19 DNA in the presence of the (.)OH scavenger dimethyl sulfoxide (DMSO) after the decay of (125)I (1) in proximity to DNA after minor-groove binding ((125)I-iod...

journal_title：Radiation research

authors： Kassis AI,Harapanhalli RS,Adelstein SJ

更新日期：1999-11-01 00:00:00

abstract：:Synchronous Chinese hamster ovary cells were treated in G1 or S phase with cycloheximide or procaine hydrochloride before and during heating at 43 degrees C. Cycloheximide and procaine apparently act by different mechanisms, with cycloheximide inhibiting protein synthesis and procaine hydrochloride supposedly affectin...

journal_title：Radiation research

authors： Dewey WC,Li XL,Wong RS

更新日期：1990-06-01 00:00:00

abstract：:These assessment of the consequences of irradiation on chromatin is complicated by endogenous nucleases. Isolation and prolonged storage of rat liver nuclei in buffers containing divalent metal ions activates these enzymes and promotes the degradation of chromatin. Irradiation of rat liver nuclei to dose levels of 20,...

journal_title：Radiation research

authors： Gelderblom D,Smit BJ,Böhm L

更新日期：1984-08-01 00:00:00

abstract：:Gleevec, a selective tyrosine kinase inhibitor, retarded the growth of anaplastic thyroid cancer cell lines in vitro and in vivo through selective inhibition of ABL tyrosine kinase activity. In the present study, we investigated the ability of Gleevec to modulate the in vitro and in vivo radiation response of anaplast...

journal_title：Radiation research

authors： Podtcheko A,Ohtsuru A,Namba H,Saenko V,Starenki D,Palona I,Sedliarou I,Rogounovitch T,Yamashita S

更新日期：2006-01-01 00:00:00

abstract：:These experiments were designed to study the kinetics and magnitude of cell repair and repopulation in tissues whose damage results in the tumor bed effect. The right hind thighs of mice were irradiated with single doses or two equal gamma-ray fractions. Interfraction intervals ranging from 30 min to 24 h (to measure ...

journal_title：Radiation research

authors： Terry NH,Ang KK,Hunter NR,Milas L

更新日期：1988-06-01 00:00:00

abstract：:Base damage flanking a radiation-induced DNA double-strand break (DSB) may contribute to DSB complexity and affect break repair. However, to date, an isolated radiation-induced DSB has not been assessed for such structures at the molecular level. In this study, an authentic site-specific radiation-induced DSB was prod...

journal_title：Radiation research

authors： Datta K,Jaruga P,Dizdaroglu M,Neumann RD,Winters TA

更新日期：2006-11-01 00:00:00

abstract：:In a scenario involving a nuclear detonation during war or a terrorist attack, acute radiation exposure combined with thermal and blast effects results in severe skin injury. Although the cutaneous injury in such a scenario may not be lethal, it may lead to inflammation, delayed wound healing and loss of the skin barr...

journal_title：Radiation research

authors： Albrecht H,Yang HY,Kiuru M,Maksaereekul S,Durbin-Johnson B,Wong MS,Stevenson TR,Rocke DM,Isseroff RR

更新日期：2018-04-01 00:00:00

abstract：:We have been investigating radiation-induced neoplastic transformants of C3H 10T1/2 mouse fibroblasts for evidence of heritable changes. C3H 10T1/2 cells were treated with 8 Gy X rays. After approximately 8 weeks of culture, type II/III foci were isolated from the monolayer using cloning rings. Cell lines developed fr...

journal_title：Radiation research

authors： Crompton NE,Sigg M,Jaussi R

更新日期：1994-04-01 00:00:00

abstract：:Dose-response relationships for X-ray-induced reciprocal translocations in spermatogonial stem cells of mutant and wild-type mice were established by spermatocyte analysis many cell generations after irradiation. The mutants studied were Wv/+, the viable allele of dominant spotting in the heterozygous state, and Slcon...

journal_title：Radiation research

authors： van Buul PP,Schöppink K,de Rooij DG,Seelen MC

更新日期：1994-02-01 00:00:00

abstract：:In higher eukaryotes, DNA double-strand breaks (DSBs) induced by ionizing radiation activate checkpoints that delay progression through the cell cycle. Compared to delays in other phases of the cell cycle, delays induced in G(2) are longer and frequently correlate with resistance to killing by radiation. Therefore, mo...

journal_title：Radiation research

authors： Fan X,Cheong N,Iliakis G

更新日期：2010-10-01 00:00:00

abstract：:The hypothesis of boron neutron capture therapy (BNCT) research has been that the short-range, high-linear energy transfer radiation produced by the capture of thermal neutrons by (10)B will potentially control tumor and spare normal tissue only if the boron compound selectively targets tumor tissue within the treatme...

journal_title：Radiation research

authors： Trivillin VA,Heber EM,Nigg DW,Itoiz ME,Calzetta O,Blaumann H,Longhino J,Schwint AE

更新日期：2006-08-01 00:00:00

abstract：:Dose-response relationships observed in laboratory animals can be used to identify possible human risk factors and may also be used in a quantitative manner when human data are not available. This paper presents an analysis of the dose dependency of osteosarcoma incidence in beagle dogs given a single inhalation expos...

journal_title：Radiation research

authors： Shyr LJ,Muggenburg BA

更新日期：1992-10-01 00:00:00

abstract：:We measured the production of reactive hydroxyl radical (OH.) by Fe2+ itself or complexed with nucleotide triphosphates or tripolyphosphate (TPP). Coumarin-3-carboxylic acid (3-CCA) reacts with the OH. produced by Fe2+, Fe3+ or Cu2+ plus ascorbate and with various iron complexes. We measured in real time the increased...

journal_title：Radiation research

authors： Biaglow JE,Held KD,Manevich Y,Tuttle S,Kachur A,Uckun F

更新日期：1996-05-01 00:00:00

abstract：:Here we report that suramin sensitizes LM217, MDA-MB-468, T98G and A431 cells to ionizing radiation. Suramin sensitized cells to X radiation in a dose-dependent fashion, and longer exposure to suramin before X irradiation resulted in more efficient sensitization. The dose-modifying factors calculated from the survival...

journal_title：Radiation research

authors： Hosoi Y,Matsumoto Y,Enomoto A,Morita A,Green J,Nakagawa K,Naruse K,Suzuki N

更新日期：2004-09-01 00:00:00

abstract：:Synthetic lysophospholipids represent a variety of analogs of the naturally occurring 2-lysophosphatidylcholine. Some of these compounds showed significant therapeutic effects on the survival of mice following radiation injury when administered after various doses of whole-body X irradiation. Such therapeutic effects ...

journal_title：Radiation research

authors： Berdel WE,Schick P,Sedlmeier H,Fink U,Rastetter J,Messerschmidt O

更新日期：1983-04-01 00:00:00

abstract：:Previous work by our group and others has shown the modulation of human immunodeficiency virus (HIV) promoter or long terminal repeat (LTR) after exposure to neutrons and ultraviolet radiations. Using HeLa cells stably transfected with a construct containing the chloramphenicol acetyl transferase (CAT) gene, the trans...

journal_title：Radiation research

authors： Libertin CR,Panozzo J,Groh KR,Chang-Liu CM,Schreck S,Woloschak GE

更新日期：1994-10-01 00:00:00

abstract：:Exposure to ionizing radiation or a variety of chemical agents is known to increase the risk of developing malignancy and many tumors have been linked to inflammatory processes. In most studies, the potentially harmful effects of ionizing radiation or other agents are considered in isolation, mainly due to the large n...

journal_title：Radiation research

authors： McAllister KA,Lorimore SA,Wright EG,Coates PJ

更新日期：2012-05-01 00:00:00

abstract：:At the Swiss Institute for Nuclear Research (SIN) cancer patients are irradiated with negatively charged pi mesons using a 60-beam medical pion generator, the Piotron. A low-pressure tissue-equivalent proportional counter was used to measure absorbed dose and microdosimetric spectra. A method was developed to allow di...

journal_title：Radiation research

authors： Schuhmacher H,Menzel HG,Blattmann H,Muth H

更新日期：1985-01-01 00:00:00

abstract：:Asynchronously growing V79 cells were assayed for mutation induction following exposure to hyperthermia either immediately before or after being irradiated with 60Co gamma rays. Hyperthermia exposures consisted of either 43.5 degrees C for 30 min or 45 degrees C for 10 min. Each of these heat treatments resulted in a ...

journal_title：Radiation research

authors： Banerjee S,Bhaumik G,Bhattacharjee SB

更新日期：1989-08-01 00:00:00

abstract：:The molecular structure of mutants induced in human lymphoblast cells by 500 cGy X rays in the presence of the radioprotector cysteamine (25 mM) has been compared with that induced by an equally mutagenic treatment of 150 cGy X rays alone. Sets of mutants at the hypoxanthine-guanine phosphoribosyl transferase locus we...

journal_title：Radiation research

authors： Liber HL,Benforado K,Little JB

更新日期：1989-05-01 00:00:00

abstract：:Studies have causally linked external thyroid radiation exposure in childhood with thyroid cancer. In 1995, investigators conducted relative risk analyses of pooled data from seven epidemiologic studies. Doses were mostly <10 Gy, although childhood cancer therapies can result in thyroid doses >50 Gy. We pooled data fr...

journal_title：Radiation research

authors： Veiga LH,Holmberg E,Anderson H,Pottern L,Sadetzki S,Adams MJ,Sakata R,Schneider AB,Inskip P,Bhatti P,Johansson R,Neta G,Shore R,de Vathaire F,Damber L,Kleinerman R,Hawkins MM,Tucker M,Lundell M,Lubin JH

更新日期：2016-05-01 00:00:00

abstract：:Polonium-210 is a naturally occurring radioactive element that decays by emitting an alpha particle. It is in the air we breathe and also a component of tobacco smoke. Polonium-210 is used as an anti-static device in printing presses and gained widespread notoriety in 2006 after the poisoning and subsequent death of a...

journal_title：Radiation research

authors： Boice JD Jr,Cohen SS,Mumma MT,Ellis ED,Cragle DL,Eckerman KF,Wallace PW,Chadda B,Sonderman JS,Wiggs LD,Richter BS,Leggett RW

更新日期：2014-02-01 00:00:00

abstract：:The monohydroxylated fatty acid content of peripheral blood mononuclear cells from 23 cleanup workers and 16 unexposed individuals was studied in relation to their immune status after the Chernobyl accident. Men with absorbed doses below 0.32 Gy showed higher levels of free and esterified 12-hydroxyeicosatetraenoic ac...

journal_title：Radiation research

authors： Chumak A,Thevenon C,Gulaya N,Guichardant M,Margitich V,Bazyka D,Kovalenko A,Lagarde M,Prigent AF

更新日期：2001-11-01 00:00:00

abstract：:A recently developed method for the direct measurement of the restricted dose mean LET (cutoff energy = 500 eV) of an unspecified photon or electron radiation field with the high-pressure ionization chamber has been utilized to investigate the variation of this radiobiologically important parameter outside the primary...

journal_title：Radiation research

authors： Makrigiorgos G,Antonadou D

更新日期：1987-04-01 00:00:00

abstract：:While nontargeted and low-dose effects such as the bystander effect are now accepted, the mechanisms underlying the response have yet to be elucidated. It has been shown that the transfer of irradiated cell conditioned medium (ICCM) can kill cells that are not directly irradiated; however, to date the effect of ICCM c...

journal_title：Radiation research

authors： Ryan LA,Smith RW,Seymour CB,Mothersill CE

更新日期：2008-02-01 00:00:00

abstract：:The incidence of micronuclei was evaluated to assess the induction of an adaptive response to non-ionizing radiofrequency (RF) radiation in peripheral blood lymphocytes collected from five different human volunteers. After stimulation with phytohemagglutinin for 24 h, the cells were exposed to an adaptive dose of 900 ...

journal_title：Radiation research

authors： Sannino A,Sarti M,Reddy SB,Prihoda TJ,Vijayalaxmi,Scarfì MR

更新日期：2009-06-01 00:00:00