Abstract：
:Base damage flanking a radiation-induced DNA double-strand break (DSB) may contribute to DSB complexity and affect break repair. However, to date, an isolated radiation-induced DSB has not been assessed for such structures at the molecular level. In this study, an authentic site-specific radiation-induced DSB was produced in plasmid DNA by triplex forming oligonucleotide-targeted (125)I decay. A restriction fragment terminated by the DSB was isolated and probed for base damage with the E. coli DNA repair enzymes endonuclease III and formamidopyrimidine-DNA glycosylase. Our results demonstrate base damage clustering within 8 bases of the (125)I-targeted base in the DNA duplex. An increased yield of base damage (purine > pyrimidine) was observed for DSBs formed by irradiation in the absence of DMSO. An internal control fragment 1354 bp upstream from the targeted base was insensitive to enzymatic probing, indicating that the damage detected proximal to the DSB was produced by the (125)I decay that formed the DSB. Gas chromatography-mass spectrometry identified three types of damaged bases in the approximately 32-bp region proximal to the DSB. These base lesions were 8-hydroxyguanine, 8-hydroxyadenine and 5-hydroxycytosine. Finally, evidence is presented for base damage >24 bp upstream from the (125)I-decay site that may form via a charge migration mechanism.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Datta K,Jaruga P,Dizdaroglu M,Neumann RD,Winters TAdoi
10.1667/RR0628.1subject
Has Abstractpub_date
2006-11-01 00:00:00pages
767-81issue
5eissn
0033-7587issn
1938-5404pii
RR0628journal_volume
166pub_type
杂志文章abstract：:Given the distinct characteristics of low-energy electrons (LEEs), particularly at energies less than 30 eV, they can be applied to a wide range of therapeutic modalities to improve cancer treatment. LEEs have been shown to efficiently produce complex molecular damage resulting in substantial cellular toxicities. Sinc...
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pub_type： 杂志文章,评审
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journal_title：Radiation research
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doi：
更新日期：1991-02-01 00:00:00
abstract：:Ionization generates a large number of secondary low-energy electrons (LEEs) with a most probable energy of approximately 10 eV, which can break DNA bonds by dissociative electron attachment (DEA) and lead to DNA damage. In this study, we investigated radiation damage to dry DNA induced by X rays (1.5 keV) alone on a ...
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journal_title：Radiation research
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doi：
更新日期：1998-09-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
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更新日期：2018-12-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
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更新日期：2018-09-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RADE-20-00102.1
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2020-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2000-05-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
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更新日期：2004-07-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
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doi：
更新日期：1999-09-01 00:00:00
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journal_title：Radiation research
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doi：
更新日期：1989-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
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更新日期：2017-06-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3362.1
更新日期：2005-10-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:Radiation-induced cell death is thought to be caused by nuclear DNA damage that cannot be repaired. However, in this study we found that a delayed increase of mitochondrial reactive oxygen species (ROS) is responsible for some of the radiation-induced cell death in normal human fibroblast cells. We have previously rep...
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更新日期：2015-04-01 00:00:00
