Abstract:
:Human keratinocytes show a bystander effect when exposed to low doses of low-LET radiation. In this paper, data are presented showing a method of correcting the overall survival curve to enable analysis of the relative contributions of the bystander effect and the effect attributable to direct interaction of the radiation with the target cell. The technique used is to obtain a standard clonogenic survival curve using the assay of Puck and Marcus and, with a different set of flasks containing cloning densities of unirradiated cells, to assay the cell killing caused by medium harvested from 2 x 10(5) cells irradiated with the same doses. The data show that for this human epithelial cell line, doses of 0.01-0. 5 Gy show clonogenic death by the bystander effect only, if maximum potential bystander killing is assumed. The magnitude of the effect is relatively constant, and it appears to saturate at doses in the range of 0.03-0.05 Gy. After doses greater than 0.5 Gy, the curves for clonogenic death are the result of a dose-dependent non-bystander effect and a dose-independent bystander effect. If these particular dose-response effects occur in epithelial cells in vivo, they may have important consequences for therapy and studies of low-dose risk.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Seymour CB,Mothersill Cdoi
10.1667/0033-7587(2000)153[0508:rcobat]2.0.co;2subject
Has Abstractpub_date
2000-05-01 00:00:00pages
508-11issue
5 Pt 1eissn
0033-7587issn
1938-5404journal_volume
153pub_type
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