Abstract:
:Our goal was to determine whether esophageal progenitor cells could be isolated from adult mouse esophagus or bone marrow and shown to home to and proliferate in the irradiated esophagus of recipient mice. Esophageal progenitor cells were isolated from adult male C3H/HeNsd or C57BL/6J green fluorescent protein (GFP(+)) mice by a serial in vitro preplate technique or the technique of side population cell sorting. When injected intravenously (i.v.), these cells homed to the 30-Gy-irradiated esophagus of GFP(-) female recipient mice and formed donor-origin esophageal foci. GFP(+) whole murine bone marrow cells injected i.v. also formed donor-origin esophageal squamous cell foci and protected recipient GFP(-) mice from upper-body irradiation in a cell dose-dependent manner. Marrow chimeric GFP(-) mice reconstituted with GFP(+) cells showed migration of GFP(+) marrow cells to the esophagus after 30 Gy irradiation. Purified esophageal progenitor cells isolated from first-generation preplate cell recipients engrafted after i.v. injection to the esophagus of second-generation-irradiated recipient mice. These data establish that esophageal progenitor cells can home to the irradiated esophagus and show limited differentiation capacity to squamous epithelium.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Epperly MW,Guo H,Shen H,Niu Y,Zhang X,Jefferson M,Sikora CA,Greenberger JSdoi
10.1667/rr3224subject
Has Abstractpub_date
2004-09-01 00:00:00pages
233-40issue
3eissn
0033-7587issn
1938-5404pii
RR3224journal_volume
162pub_type
杂志文章abstract::The present study was undertaken to standardize a dichlorofluorescein (DCF) assay for measurement of radiation-induced oxidation of dichlorofluorescin (DCFH) substrate in MCF-10 cells. This assay was highly sensitive and capable of detecting increased DCFH oxidation in the cells exposed to gamma radiation at doses as ...
journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
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journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2019-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3053
更新日期:2003-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2999.1
更新日期:2013-02-22 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-04-01 00:00:00
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更新日期:2013-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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