Abstract:
:To evaluate whether DNA alterations in mature spermatozoa could stem from DNA damage induced in immature germ cells, testis cells and spermatozoa were analyzed by the comet assay and by the sperm chromatin structure assay 14, 45 and 100 days after in vivo X irradiation of the testes. These times were selected, according to the mouse seminiferous epithelium cycle, to follow the DNA damage induced in different germ cell compartments. The cytotoxic action was assessed by DNA flow cytometric analysis of testicular cells. A dose-dependent increase of DNA damage in testis cells was observed 14 days after irradiation, whereas mature sperm cells were not affected. On the other hand, an increase in DNA strand breaks was seen in spermatozoa 45 days after treatment. DNA damage returned to the control levels 100 days after irradiation. The methods used to evaluate DNA damage gave comparable results, emphasizing the correlation between DNA fragmentation and susceptibility of sperm chromatin to denaturation. Both techniques showed the high radiosensitivity of differentiating spermatogonia. The overall results showed that DNA damage induced in pre-meiotic germ cells is detectable in primary spermatocytes and is still present in mature spermatozoa.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Cordelli E,Fresegna AM,Leter G,Eleuteri P,Spanò M,Villani Pdoi
10.1667/rr3053subject
Has Abstractpub_date
2003-10-01 00:00:00pages
443-51issue
4eissn
0033-7587issn
1938-5404pii
RR3053journal_volume
160pub_type
杂志文章abstract::In a recent study (Int. J. Radiat. Oncol. Biol. Phys. 36, 635-639, 1996), 1H nuclear magnetic resonance (NMR) spectroscopy was used to demonstrate significant decreases in lactate levels after gamma irradiation of radiosensitive RIF-1 tumors in vitro. For comparison, we have examined the effects of gamma radiation on ...
journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::We previously reported that a priming dose of 0.3 Gy on gestation day 11 significantly increased the rate of living fetuses and reduced the incidence of congenital malformations caused by exposure to 5 Gy X rays on gestation day 12 in ICR mice. In the present study, postnatal development of the live offspring was inve...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::Irradiation from internally deposited radionuclides induces malignant tumors. Ingested long-lived radionuclides accumulate in specific organs that are irradiated throughout life. To elucidate why the development of malignant tumors requires long-term internal exposure, of the order of decades, despite the fact that ir...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1492.1
更新日期:2009-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::A radiation-induced bystander response, which is generally defined as a cellular response that is induced in nonirradiated cells that received bystander signals from directly irradiated cells within an irradiated cell population. In our earlier X-ray microbeam studies, bystander cell killing in normal human fibroblast...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR3010.1
更新日期:2013-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::The structural maintenance of chromosome 1 (Smc1) protein is a member of the highly conserved cohesin complex and is involved in sister chromatid cohesion. In response to ionizing radiation, Smc1 is phosphorylated at two sites, Ser-957 and Ser-966, and these phosphorylation events are dependent on the ATM protein kina...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2402.1
更新日期:2011-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR3236.1
更新日期:2013-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/RR15553.1
更新日期:2020-03-01 00:00:00
abstract::Synchronous Chinese hamster ovary cells were treated in G1 or S phase with cycloheximide or procaine hydrochloride before and during heating at 43 degrees C. Cycloheximide and procaine apparently act by different mechanisms, with cycloheximide inhibiting protein synthesis and procaine hydrochloride supposedly affectin...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::The effect of extremely low-frequency electromagnetic field (ELF EMF) exposures to human health has been widely debated. Epidemiological studies have found a possible correlation between increased cancer incidence and environmental ELF EMF exposures. Results from in vitro studies performed to examine the possible unde...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0663:eodctl]2.0.co;2
更新日期:2000-05-01 00:00:00
abstract::Radiation-induced neurotoxicity is a well-characterized phenomenon. However, the underlying mechanism of this toxicity is poorly understood. In the central nervous system (CNS), excitotoxic mechanisms are implicated in many neurodegenerative disease processes. Pivotal to the excitotoxic pathway is dysfunction of gluta...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2106.1
更新日期:2010-12-01 00:00:00
abstract::The primary risk factors of multiple myeloma are age, race and sex, but several studies have found an association between radiological hazards and multiple myeloma. The purpose of this nested case-control study was to investigate whether workers with chronic low-level exposure to internally deposited uranium at the Oa...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1607.1
更新日期:2009-06-01 00:00:00
abstract::Radon is recognized as a public health concern for indoor exposure. Precise quantification derived from occupational exposure in miners is still needed for estimating the risk and the factors that modify the dependence on cumulated exposure. The present paper reports on relationship between radon exposure and lung can...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0848.1
更新日期:2008-02-01 00:00:00
abstract::The human breast is sensitive to radiation carcinogenesis, and genomic instability occurs early in breast cancer development. This study tests the hypothesis that ionizing radiation elicits genomic instability in finite life-span human mammary epithelial cells (HMEC) and asks whether densely ionizing radiation is a mo...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1317.1
更新日期:2008-07-01 00:00:00
abstract::We present theoretical arguments to explain observations of a "reversal" of the RBE at relatively large doses; that is, the RBE of high-LET vs low-LET radiation is less than one. Numerical examples are given and the results of Bogo et al. (Radiat. Res. 118, 341-352, 1989) are discussed qualitatively. ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::The mortality experience between 1956 and 1985 of 8977 males employed by Atomic Energy of Canada Limited is reported. A total of 4260 men, 47% of the cohort, were exposed to low doses of external ionizing radiation at low dose rates, with a mean cumulative equivalent dose of 52.1 mSv. For cancers as a whole the excess...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::This experiment was performed to determine whether ionizing radiation is essential for maximum growth rate in a ciliated protozoan. When extraneous ionizing radiation was reduced to 0.15 mrad/day, the reproduction rate of Tetrahymena pyriformis was significantly less (P less than 0.01) than it was at near ambient leve...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1128.1
更新日期:2007-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Induction of interphase death was examined in Chinese hamster ovary cells exposed to accelerated heavy ions (carbon, neon, argon and iron) of various linear energy transfers (LETs) (10-2000 keV/microm). The fraction of cells that underwent interphase death was determined by observing individual cells with time-lapse p...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::The non-random distribution of DNA breakage in PFGE (pulsed-field gel electrophoresis) experiments poses a problem of proper subtraction of the background DNA damage to obtain a fragment-size distribution due to radiation only. A naive bin-to-bin subtraction of the background signal will not result in the right DNA ma...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0663.1
更新日期:2006-12-01 00:00:00
abstract::Evaluation of the probability of biological phenomena per cell by colony formation, requires knowledge not only of the number of cells at risk but also of their microdistribution. In the present study, the influence of cellular multiplicity (number of cells per potential colony-forming unit) on the determination of ra...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::If the limited life span of hematopoietic tissues in vitro is due to a finite proliferative capacity of individual stem cells, one might expect tissues of young donors to possess a greater proliferative capacity and to contain a larger population of primitive stem cells than those of older donors. To test this hypothe...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::The natural survival, relative to properly chosen controls, of 26 beagle dogs injected once intravenously with an average of 0.58 +/- 0.04 kBq 239Pu/kg, 23 dogs injected with 2.31 +/- 0.43 kBq 226Ra/kg, 13 dogs injected with 1.84 +/- 0.26 kBq 228Ra/kg, 12 dogs injected with 0.56 +/- 0.030 kBq 228Th/kg, and 12 dogs inj...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::To establish the tolerance of liver tissue to single high-dose intraoperative irradiation, the histopathological changes in the canine liver after single high-dose intraoperative irradiation were investigated by means of radionuclide imaging and light microscopy. Intraoperative irradiation at doses of 0, 10, 20, 25 or...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)154[0537:tdashd]2.0.co;2
更新日期:2000-11-01 00:00:00