Abstract:
:The effect of extremely low-frequency electromagnetic field (ELF EMF) exposures to human health has been widely debated. Epidemiological studies have found a possible correlation between increased cancer incidence and environmental ELF EMF exposures. Results from in vitro studies performed to examine the possible underlying bioeffects of ELF EMFs have varied greatly. Reported effects range from robust and reproducible effects to undetectable. In this study, Daudi cells were exposed to 60 Hz magnetic fields for 20, 40 or 60 min at flux densities of 12.5, 50, 100 or 500 microT. Exposures were performed in the Regional ELF-EMF Exposure Facility (Rockville, MD) to minimize variables that might contribute to a false positive effect. Exposures included sham/sham, exposed/sham or sham/exposed, and were performed with blinding with respect to type of exposure. 12-O-Tetradecanoylphorbol-13-acetate (TPA) treatment was used as a positive control. Total cellular RNA was isolated using a single-step technique. Human MYC expression was measured by northern blot hybridization as an indicator of the responsiveness of Daudi cells to experimental conditions. Beta-2-microglobulin (B2M) expression was measured simultaneously as an internal control. Exposure to a 60 Hz magnetic field did not significantly alter MYC expression in Daudi cells under any of the exposure conditions.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Morehouse CA,Owen RDdoi
10.1667/0033-7587(2000)153[0663:eodctl]2.0.co;2subject
Has Abstractpub_date
2000-05-01 00:00:00pages
663-9issue
5 Pt 2eissn
0033-7587issn
1938-5404journal_volume
153pub_type
杂志文章abstract::The relationships between DNA damage and the survival of murine bone marrow cells irradiated in situ were examined. Cell survival was assayed by the ability of bone marrow cells from irradiated mice to form colonies in vitro (CFU-C). DNA double-strand breaks (DSBs) were measured by neutral (nondenaturing) filter eluti...
journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1992-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1983-04-01 00:00:00
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journal_title:Radiation research
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更新日期:2007-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1667/rr3236
更新日期:2004-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1962-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)154[0640:aogmpb]2.0.co;2
更新日期:2000-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1667/RR0535.1
更新日期:2006-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0628.1
更新日期:2006-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:1987-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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