Abstract：
:We studied heat-induced apoptosis and loss of clonogenicity in Rat-1 fibroblasts, thermotolerant Rat-1 (TT Rat-1), Rat-1 transfected with the human hsp-70 gene (M21) and Rat-1 transfected with the human c-myc proto-oncogene (Rat-1:myc). Relative to Rat-1, TT Rat-1 and M21 cells are heat-resistant, but Rat-1:myc cells are heat-sensitive, in terms of both apoptosis and clonogenic survival. The apoptotic fractions assayed 24 h after a heat treatment of 60 min at 44 degrees C, are about 20% for Rat-1, 7% for TT Rat-1, 10% for M21 and 70% for Rat-1:myc cells, respectively. Most of the apoptotic cells detach from substratum within 1 day of heat treatment and exhibit morphological changes, chromatin condensation and DNA fragmentation. The results of this study suggest that (1) apoptosis is an important mechanism of heat-induced cell killing in some cell lines, (2) apoptosis-mediated cell killing manifests rapidly (relative to clonogenic assay) after a heat treatment, and (3) overexpression of the human hsp-70 gene reduces, whereas expression of the human c-myc proto-oncogene enhances, heat-induced apoptosis. Lastly, the effects of the hsp-70 and c-myc genes on the thermosensitivity of cells are correlated with their modulation of apoptosis.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Li WX,Chen CH,Ling CC,Li GCsubject
Has Abstractpub_date
1996-03-01 00:00:00pages
324-30issue
3eissn
0033-7587issn
1938-5404journal_volume
145pub_type
杂志文章abstract：:Exposure of humans and rodents to radiofrequency (RF) cell phone fields has been reported to alter a number of stress- related parameters. To study this potential relationship in more detail, tube-restrained immobilized Fischer 344 rats were exposed in the near field in a dose-dependent manner to pulse-modulated (11 p...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0584:eoiace]2.0.co;2
更新日期：2001-04-01 00:00:00
abstract：:To determine whether exposure to radiofrequency (RF) radiation can induce DNA damage or apoptosis, Molt-4 T lymphoblastoid cells were exposed with RF fields at frequencies and modulations of the type used by wireless communication devices. Four types of frequency/modulation forms were studied: 847.74 MHz code-division...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3127
更新日期：2004-02-01 00:00:00
abstract：:An induction-type radiofrequency generator was used to heat thigh implants of the VX2 rabbit carcinoma. The tumor temperature could be easily raised to over 50 degrees C, while the temperature of normal adjacent muscle generally remained at about 43 degrees C. The marked hypovascularity of the tumor, as demonstrated a...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-02-01 00:00:00
abstract：:In the event of a radiation accident or attack, it will be imperative to quickly assess the amount of radiation exposure to accurately triage victims for appropriate care. RNA-based radiation dosimetry assays offer the potential to rapidly screen thousands of individuals in an efficient and cost-effective manner. Howe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr1977.1
更新日期：2011-02-01 00:00:00
abstract：:We present measurements of low-energy (<18 eV) electron-stimulated desorption of anions from acetamide (CH(3)CONH(2)) and dimethyl disulfide [DMDS: (CH(3)S)(2)] films. Electron irradiation of physisorbed CH(3)CONH(2) produces H(-), CH(3)(-) and O(-) anions, whereas the H(-), CH(2)(-), CH(3)(-), S(-), SH(-) and SCH(3)(...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0023:aopsib]2.0.co;2
更新日期：2002-07-01 00:00:00
abstract：:The relationship between the intracellular glutathione (GSH) concentration and the aerobic radiation response was studied in Chinese hamster ovary cells. Various degrees of GSH depletion were produced by exposure to buthionine sulfoximine (BSO) and/or diethyl maleate (DEM). Diethyl maleate did not act as a classical r...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:The relative biological effectiveness was determined using sex-linked recessive lethals induced in Drosophila spermatozoa as the biological effect. The sex-linked recessive lethal test, a measure of mutations induced in germ cells and transmitted through successive generations, yields a linear dose-response curve in t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Accurate assessment of risks of long-term space missions is critical for human space exploration. It is essential to have a detailed understanding of the biological effects on humans living and working in deep space. Ionizing radiation from galactic cosmic rays (GCR) is a major health risk factor for astronauts on ext...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15062.1
更新日期：2018-06-01 00:00:00
abstract：:Wistar rats (36) were exposed daily throughout pregnancy to a power density level of 35 mW/cm2 of 6000-MHz microwave radiation (11), sham irradiated (10), or used as control animals (15). Litters were culled to a maximum of eight F1a offspring/litter (total = 124) on Postnatal Day 1 and subjected to a series of reflex...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-02-01 00:00:00
abstract：:There is growing scientific, regulatory and public concern over anthropogenic input of radionuclides to the aquatic environment, especially given the issues surrounding existing nuclear waste, future energy demand and past or potential nuclear accidents. A change in the approach to how we protect the environment from ...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/rr2687.1
更新日期：2012-05-01 00:00:00
abstract：:A subline of a rat mammary carcinoma (MATB 13762), selected for resistance to melphalan, is cross-resistant to other alkylating drugs, to unrelated drugs and to ionizing radiation. The difference in radioresponse between the sensitive wild-type cell line and the melphalan- and radiation-resistant line (MLNr) is relate...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:To approach treatment of micrometastases of steroid receptor-rich cancers using estrogen receptor-directed therapy with Auger electrons, multicellular spheroids of the estrogen receptor-rich human breast cancer cell line, MCF-7, were prepared and exposed to a range of concentrations of an Auger electron-emitting estro...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:The effect of ionizing radiation on the survival of bovine aortic endothelial (BAE) cells was determined by the in vitro colony formation method. The BAE cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% calf serum, antibiotics, and growth factors obtained from the culture of mouse...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：:It is well known that ionizing radiation-induced toxicity to normal tissue has functional consequences in the brain. However, the underlying molecular alterations have yet to be elucidated. We have previously reported cognitive impairments with concomitant changes in dendritic complexity, spine density and inflammatio...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15061.1
更新日期：2018-11-01 00:00:00
abstract：:Mixed radiation fields comprise the most common form of radiation exposure. Given their relevance in radiation protection, cancer radiotherapy and space research, accurate predictions of the corresponding radiation effects are essential. The local effect model (LEM) allows the prediction of cell survival after ion irr...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15456.1
更新日期：2020-02-01 00:00:00
abstract：:Irradiation of human melanoma (MeWo, Be11) and squamous cell carcinoma (4451, 4197) cells induces cell cycle blocks from which the cells recover to re-enter mitosis after 40-60 h. In the TP53 mutant cell lines, MeWo and 4451, irradiation induces a G(2)-phase block, where the fraction of cells in G(2) phase reaches a m...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)154[0640:aogmpb]2.0.co;2
更新日期：2000-12-01 00:00:00
abstract：:The purpose of this paper is to validate a metabolic model describing the kinetics of tritium in man. The validation is based on measurements of background levels of loose and bound tritium in Italian subjects and their diets. Model predictions are compared with empirical measurements of tritium in human urine and tis...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:Statistical analysis of Monte Carlo simulations of positive ion track structure is presented for 1 MeV protons passing outside an absorbing region of interest. Energy deposition and production of ionization within the region occur only by delta-ray transport from the ion's path. Depositions in spherical absorber regio...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:Two different sets of Monte Carlo computations were carried out for the study of dose penetration of monoenergetic, low-energy (10 to 100 keV) photon beams incident on slabs of tissue. One program took into account coherent scattering and considered electron binding when finding the angle of scattering during incohere...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Induction of interphase death was examined in Chinese hamster ovary cells exposed to accelerated heavy ions (carbon, neon, argon and iron) of various linear energy transfers (LETs) (10-2000 keV/microm). The fraction of cells that underwent interphase death was determined by observing individual cells with time-lapse p...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:The effect of sodium bisulfite, a specific inhibitor of chromatin proteolysis, on radiation damage in rat thymocytes in vitro was examined. Rat thymocytes irradiated with 1 kR X rays in vitro were incubated at 37 degrees C with 10 mM glucose for 4 to 6 hr. During that time development of interphase death as judged by ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:Numerous studies have shown that histone deacetylase inhibitors (HDACis) improve cellular acetylation while also enhancing the radiation sensitivity. In this work, however, we confirmed that low-dose trichostatin A (TSA) as a typical HDACi could reduce rather than increase the radiosensitivity of cancer cells, while t...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RADE-20-00145.1
更新日期：2021-01-29 00:00:00
abstract：:Ketogenic diets are low in carbohydrates and high in fat, which forces cells to rely more heavily upon mitochondrial oxidation of fatty acids for energy. Relative to normal cells, cancer cells are believed to exist under a condition of chronic mitochondrial oxidative stress that is compensated for by increases in gluc...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14668.1
更新日期：2017-06-01 00:00:00
abstract：:To characterize the differences in the radiosensitivity of individual populations of human hematopoietic stem/progenitor cells (HSPCs), we examined the relationship among cell surface antigens, clonogenic potential and radiation survival. The expressions of CD34, CD38, CD45RA, CD110 and Tie-2, early differentiation pa...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1839.1
更新日期：2010-02-01 00:00:00
abstract：:Aberrant expression of microRNAs (miRNAs) is frequently associated with a variety of cancers, including breast cancer. We and others have demonstrated that radiation-induced rat mammary cancer exhibits a characteristic gene expression profile and a random increase in aberrant DNA copy number; however, the role of aber...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2927.1
更新日期：2013-02-01 00:00:00
abstract：:1. A survival curve for the colony-forming ability of mouse bone marrow cells irradiated in vivo with Co60 gamma-rays has been obtained. The curve may be characterized by D0 of 95 rads and an extrapolation number of 1.5. 2. Comparison with the survival curve obtained for irradiated in vitro indicated a statistically s...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1962-06-01 00:00:00
abstract：:Human immortalized keratinocytes were exposed to a range of single or fractionated doses of gamma rays from (60)Co, to medium harvested from donor cells exposed to these protocols, or to a combination of radiation and irradiated cell conditioned medium (ICCM). The surviving fractions after direct irradiation or exposu...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0626:badeaf]2.0.co;2
更新日期：2002-11-01 00:00:00
abstract：:Blockers of the renin-angiotensin-aldosterone system (RAAS) ameliorate cognitive deficits and some aspects of brain injury after whole-brain irradiation. We investigated whether treatment with the angiotensin II type 1 receptor antagonist L-158,809 at a dose that protects cognitive function after fractionated whole-br...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2560.1
更新日期：2011-07-01 00:00:00
abstract：:The Radiological Research Accelerator Facility (RARAF) is in its 50th year of operation. It was commissioned on April 1, 1967 as a collaboration between the Radiological Research Laboratory (RRL) of Columbia University, and members of the Medical Research Center of Brookhaven National Laboratory (BNL). It was initiall...
journal_title：Radiation research
pub_type： 历史文章,杂志文章
doi：10.1667/RR002CC.1
更新日期：2017-04-01 00:00:00
abstract：:Deep-space travel presents risks of exposure to ionizing radiation composed of a spectrum of low-fluence protons (1H) and high-charge and energy (HZE) iron nuclei (e.g., 56Fe). When exposed to galactic cosmic rays, each cell in the body may be traversed by 1H every 3-4 days and HZE nuclei every 3-4 months. The effects...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14667.1
更新日期：2017-08-01 00:00:00
