Abstract:
:Radiation-induced neurotoxicity is a well-characterized phenomenon. However, the underlying mechanism of this toxicity is poorly understood. In the central nervous system (CNS), excitotoxic mechanisms are implicated in many neurodegenerative disease processes. Pivotal to the excitotoxic pathway is dysfunction of glutamate signaling. We reported previously that exposure to low-LET γ radiation results in altered glutamate transport in neurons and astrocytes. In the present study, we sought to investigate the effects of various particle radiations of differing LET on glutamate transport as a measure of the neurochemical vulnerability of the CNS. NTera2-derived neurons and astrocytes isolated as pure and mixed cultures were exposed to doses of 10 cGy, 50 cGy or 2 Gy of 250 MeV protons, 290 MeV/nucleon carbon ions, or 1000 MeV/nucleon iron ions. Transporter function was assessed at 3 h, 2 days and 7days after exposure. Functional assessment of glutamate transport revealed that neurons and astrocytes respond in a reciprocal manner after exposure to particle radiation. Uptake activity in neurons increased after particle irradiation. This effect was evident as late as our last time (7 days) after exposure (P < 0.05). In astrocytes, transporter activity decreased after exposure. The decrease in uptake observed in astrocytes was evident 7 days after exposure to carbon and iron ions. Uptake in mixed cultures after exposure to all three forms of radiation revealed a muted interactive response suggestive of the individual responses of each cellular phenotype acting in opposition.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Sanchez MC,Nelson GA,Green LMdoi
10.1667/RR2106.1subject
Has Abstractpub_date
2010-12-01 00:00:00pages
669-78issue
6eissn
0033-7587issn
1938-5404pii
10.1667/RR2106.1journal_volume
174pub_type
杂志文章abstract::Caffeine was found to potentiate X-ray-induced killing of human diploid fibroblasts from a normal subject and an ataxia-telangiectasia (AT) patient when it was present at 2 mM concentration for 30 to 66 hr postirradiation. The dose-modifying factor for caffeine-treated normal cells had an average value of 1.26 +/- 0.1...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3528.1
更新日期:2006-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/RR3546.1
更新日期:2006-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)158[0626:badeaf]2.0.co;2
更新日期:2002-11-01 00:00:00
abstract::Ion microscopy was used for subcellular quantitative imaging of the isotopes 10B and 11B in the same cell to evaluate boron delivery using a mixture of two neutron capture therapy drugs, p-boronophenylalanine-fructose (BPA-F) and sodium borocaptate (BSH). The application of 10B-labeled BPA-F and 11B-labeled BSH allowe...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)157[0700:qssims]2.0.co;2
更新日期:2002-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::After total-body exposure to various doses of ionizing radiation, the ability of lymphocytes to interact specifically with high endothelial venules of rat cervical and mesenteric lymph nodes was analyzed in frozen sections. Following a radiation dose of 1.5 Gy, high endothelial venules remained intact and the binding ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::Proton exposure induces mutations and cancer, which are presumably linked. Because protons are abundant in the space environment and significant uncertainties exist for the effects of space travel on human health, the purpose of this study was to identify the types of mutations induced by exposure of mammalian cells t...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR3174.1
更新日期:2013-05-01 00:00:00
abstract::Single crystals of guanine hydrobromide monohydrate, in which the guanine base is protonated at N7, were X-irradiated at 8 and 65 K. Using K-band ESR, ENDOR, and field-swept-ENDOR (FSE) techniques, the crystals were studied between 8 K and room temperature. There was evidence for five different radicals, RI-RV, immedi...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::To determine whether different agents that enhance the expression of potentially lethal X-ray damage (PLD) interact with the same or different lesions (or spectrum of lesions), cell killing was measured in three kinds of experiments: (1) When cells were irradiated in G1 phase and treated with caffeine or hydroxyurea a...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::Based on murine survival studies, endogenous hemopoietic spleen colony formation (E-CFU), and recovery of bone marrow and splenic granulocyte-macrophage colony-forming cells (GM-CFC), it was demonstrated that the postirradiation administration of glucan, an immunomodulator and hemopoietic stimulant, enhances the radio...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr1977.1
更新日期:2011-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-07-08 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
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更新日期:2009-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
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更新日期:2008-03-01 00:00:00
abstract::The ability of the aminothiol WR-1065 [N-(2-mercaptoethyl)-1,3-diaminopropane] to protect L5178Y (LY) cells against the cytotoxic and mutagenic effects of exposure to accelerated (56)Fe ions (1.08 GeV/nucleon) was determined. It was found that while WR-1065 reduced the mutagenicity in both cell lines when it was prese...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)158[0110:aowilc]2.0.co;2
更新日期:2002-07-01 00:00:00
abstract::Radiation nephropathy is one of the common late effects in cancer survivors who received radiotherapy as well as in victims of radiation accidents. The clinical manifestations of radiation nephropathy occur months after exposure. To date, there are no known early biomarkers to predict the future development of radiati...
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pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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更新日期:2013-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00