Abstract：
:To determine whether different agents that enhance the expression of potentially lethal X-ray damage (PLD) interact with the same or different lesions (or spectrum of lesions), cell killing was measured in three kinds of experiments: (1) When cells were irradiated in G1 phase and treated with caffeine or hydroxyurea at concentrations that yield maximal response, the same survival plateaus were reached. (2) Treatment of cells irradiated in G1 phase either with caffeine or with hydroxyurea so as to yield survival levels that differed twofold after 4 h incubation, followed by treatment with caffeine to allow expression of PLD in G2 phase, resulted eventually in the same level of survival. (3) When cells were irradiated and treated with caffeine, hydroxyurea, or 9-beta-D-arabinofuranosyladenine (araA) after progressively longer delays, to trace the time course of recovery from the PLD, the responses obtained with caffeine and araA were similar; sensitivity to hydroxyurea was lost more rapidly. The results are consistent with the possibility that these three agents interact with the same lesions, but that different steps in the repair process are inhibited by caffeine or araA than by hydroxyurea.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Beetham KL,Tolmach LJsubject
Has Abstractpub_date
1991-11-01 00:00:00pages
225-8issue
2eissn
0033-7587issn
1938-5404journal_volume
128pub_type
杂志文章abstract：:The linear no-threshold extrapolation from a dose-response relationship for ionizing radiation derived at higher doses to doses for which regulatory standards are proposed is being challenged by some scientists and defended by others. It appears that the risks associated with exposures to doses of interest are below t...
journal_title：Radiation research
pub_type：
doi：
更新日期：1998-12-01 00:00:00
abstract：:The effect of X irradiation on the respiration of rat thymocytes was studied. An increase in the rate of O2 uptake was observed 1 h after cells were irradiated with doses of 6-10 Gy. The radiation-induced increase in respiration could be blocked by oligomycin, an inhibitor of mitochondrial ATP synthase, suggesting con...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Following X irradiation of 3',5'-cyclic guanosine monophosphate single crystals at 10 K, several free radicals were trapped and detected by ESR/ENDOR/FSE spectroscopy. The two dominant species both have unpaired spin located on the guanine base. One is the product of net hydrogen atom loss from the exocyclic amino gro...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Telomeres cap the ends of chromosomes, preventing end-to-end fusions and subsequent chromosome instability. Here we used a telomerase knockout model to investigate whether telomerase participates in the processes of DNA break repair by de novo synthesis of telomere repeats at broken chromosome ends (chromosome healing...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3203
更新日期：2004-08-01 00:00:00
abstract：:Solid thymidylyl-(3'-->5')-thymidine (dTpdT) was irradiated in a vacuum with monochromatic photons from 150 to 300 nm; the photoproducts were analyzed quantitatively by high-performance liquid chromatography. The results of the experiment were as follows: (1) above 210 nm the major photoproducts were three dithymine p...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:Tritium that is bound to organic molecules is of special risk for living systems, in particular when such molecules are components of the cell nucleus. Therefore, [3H]thymidine and [3H]arginine were studied for radiotoxicity in early mammalian embryo development. Starting with the two-cell stage, mouse embryos were in...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:An induction-type radiofrequency generator was used to heat thigh implants of the VX2 rabbit carcinoma. The tumor temperature could be easily raised to over 50 degrees C, while the temperature of normal adjacent muscle generally remained at about 43 degrees C. The marked hypovascularity of the tumor, as demonstrated a...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-02-01 00:00:00
abstract：:Serum copper concentration was evaluated as an index of lung injury (monitored by lung prostacyclin production) with respect to the effects of time, dose, dose fractionation, and penicillamine dose modification in rats irradiated to the right hemithorax. Both lung PGI2 production and serum Cu concentration increased w...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:The relationship between the intracellular glutathione (GSH) concentration and the aerobic radiation response was studied in Chinese hamster ovary cells. Various degrees of GSH depletion were produced by exposure to buthionine sulfoximine (BSO) and/or diethyl maleate (DEM). Diethyl maleate did not act as a classical r...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:While the link between risk of leukemia and acute radiation exposure is well established for large doses received acutely, uncertainty remains around the translation of these risk estimates to occupational exposure scenarios where the doses are low and accumulated over time, possibly over many years. We present leukem...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15358.1
更新日期：2019-11-01 00:00:00
abstract：:Keratinocyte growth factor (FGF7, also known as KGF) ameliorates the radiation response of mouse oral mucosa and other epithelial tissues. However, the precise mechanisms remain unclear. The aim of the present study was to investigate the effect of FGF7 on the survival and colony size of normal human epidermal keratin...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)156[0761:eokgfo]2.0.co;2
更新日期：2001-12-01 00:00:00
abstract：:Evaluation of the probability of biological phenomena per cell by colony formation, requires knowledge not only of the number of cells at risk but also of their microdistribution. In the present study, the influence of cellular multiplicity (number of cells per potential colony-forming unit) on the determination of ra...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Radiation-induced neurotoxicity is a well-characterized phenomenon. However, the underlying mechanism of this toxicity is poorly understood. In the central nervous system (CNS), excitotoxic mechanisms are implicated in many neurodegenerative disease processes. Pivotal to the excitotoxic pathway is dysfunction of gluta...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2106.1
更新日期：2010-12-01 00:00:00
abstract：:The relative biological effectiveness was determined using sex-linked recessive lethals induced in Drosophila spermatozoa as the biological effect. The sex-linked recessive lethal test, a measure of mutations induced in germ cells and transmitted through successive generations, yields a linear dose-response curve in t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:To examine more fully the nature of chromosomal radiosensitivity in ataxia telangiectasia (AT) cells, we employed 24-color combinatorial painting to visualize 137Cs gamma-ray-induced chromosome-type aberrations in cells of two AT and one normal primary human fibroblast strains irradiated in log-phase growth. As a meas...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3255
更新日期：2004-11-01 00:00:00
abstract：:The stable free radical 4-oxo-2,2,6,6-tetramethylpiperidinooxy (2,2,6,6-tetramethylpiperidone-N-oxyl, TEMPONE) in nitrous oxide-saturated aqueous formate solution has been shown to be a quantitative dosimeter for in situ radiolysis electron spin resonance (ESR) studies. The bleach of the TEMPONE ESR signal is proporti...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:During the pulsed-electron beam direct grafting of neat styrene onto poly(tetrafluoroethylene-co-hexafluoropropylene) (FEP) substrate, the radiolytically-produced styryl and carbon-centered FEP radicals undergo various desired and undesired competing reactions. In this study, a high-dose rate is used to impede the und...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15006.1
更新日期：2018-09-01 00:00:00
abstract：:On future missions into deep space, astronauts will be required to work more autonomously than on previous missions, and thus their ability to perform executive functions could be critical to mission success. In this study, we determined the effect that ≤15 cGy of 600 MeV/n 56Fe particles has on attentional set-shifti...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15085.1
更新日期：2018-12-01 00:00:00
abstract：:In the 400-MHz 1H NMR spectra of gamma-irradiated, N2O-saturated aqueous solutions of polyuridylic acid (polyU), polycytidylic acid (polyC), polyadenylic acid (polyA), and single-stranded DNA from calf thymus, well-resolved signals of the undamaged free bases were identified. Upon incubation of the solutions at 95 deg...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:A biphasic curve was observed when surviving allogeneic lytic activity was plotted as a function of irradiation delivered before sensitization. Flow cytometry analysis demonstrated that the number of cells was reduced in response to increasing dose and that subset precursors Lyt 1+2+ were proportionally more radiosens...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:Ionizing radiation is known to effect development during early life stages. Lake whitefish (Coregonus clupeaformis) represent a unique model organism for examining such effects. The purpose of this study was to examine how ionizing radiation affects development in lake whitefish embryos and to investigate the presence...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14574.1
更新日期：2017-10-01 00:00:00
abstract：:Chinese hamster ovary (CHO) cells were exposed to various concentrations of diethylmaleate (DEM) during a 42 degrees C incubation to determine if glutathione (GSH) compartmentalization was a factor in modification of thermal sensitivity. Cytoplasmic and mitochondrial GSH were isolated from CHO cells immediately after ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:In the double-strand break (DSB) model, the initial slope (alpha) of the survival curve is determined by the number of DSBs remaining unrepaired at a certain time after irradiation. There are three types of calculations which confirm the validity of this approach. First, the model quite reasonably predicts the low-LET...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:The dependence of relative biological effectiveness (RBE) on photon energy is a topic of extensive discussions. The increasing amount of in vitro data in the low-energy region indicates this to be a complex dependence that is influenced by the end point and cell line studied. In the present investigation, the RBE of 1...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0874.1
更新日期：2008-03-01 00:00:00
abstract：:To compare the genotoxic effects of high-LET ionizing radiation to those of low-LET radiation, we investigated the responses of human lymphoblastoid cells to DNA damage TK6 after treatment with either low-LET X rays or high-LET iron ions (1000 keV/microm). A highly localized distribution of gammaH2AX/RAD51 foci was ob...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0678:dccpih]2.0.co;2
更新日期：2002-12-01 00:00:00
abstract：:After total-body exposure to various doses of ionizing radiation, the ability of lymphocytes to interact specifically with high endothelial venules of rat cervical and mesenteric lymph nodes was analyzed in frozen sections. Following a radiation dose of 1.5 Gy, high endothelial venules remained intact and the binding ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:We have previously published the techniques and preliminary results of an SV40 viral probe assay for gamma-radiation-induced single- and double-strand DNA breaks and their intracellular repair in higher cells (Radiat. Res. 101, 356-372, 1985). Those experiments with SV40 infected CV-1 monkey kidney cells suggested tha...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:Bcl-X(S) is a pro-apoptosis member of the Bcl2 family that has been shown to induce cell death and enhance chemosensitivity. We have investigated the effect of Bcl-X(S) overexpression on radiation sensitivity. Using a tetracycline-repressible system, we found that removal of tetracycline for 16 h induced Bcl-X(S) and ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3169
更新日期：2004-05-01 00:00:00
abstract：:The effects of X irradiation on oligodendrocyte-type-2-astrocyte (O-2A) progenitor cells derived from different regions of the perinatal central nervous system (CNS) of rats were investigated in vitro. The O-2A progenitor cells can differentiate into either oligodendrocytes or type-2 astrocytes. The depletion of these...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:The ability of the aminothiol WR-1065 [N-(2-mercaptoethyl)-1,3-diaminopropane] to protect L5178Y (LY) cells against the cytotoxic and mutagenic effects of exposure to accelerated (56)Fe ions (1.08 GeV/nucleon) was determined. It was found that while WR-1065 reduced the mutagenicity in both cell lines when it was prese...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0110:aowilc]2.0.co;2
更新日期：2002-07-01 00:00:00
