Abstract:
:Keratinocyte growth factor (FGF7, also known as KGF) ameliorates the radiation response of mouse oral mucosa and other epithelial tissues. However, the precise mechanisms remain unclear. The aim of the present study was to investigate the effect of FGF7 on the survival and colony size of normal human epidermal keratinocytes in vitro. Primary neonatal keratinocytes (HEKn) were irradiated with doses of 0 and 2 Gy of 200 kV X rays and incubated in the presence or absence of 100 ng/ml FGF7. The plating efficiency (PE) and surviving fraction (SF2) were determined using a clonogenic assay. In cell cultures without FGF7, the mean PE was 4.6 +/- 0.2%. Irradiation with 2 Gy resulted in an SF2 of 51 +/- 2%. In cell cultures with FGF7, the mean PE was identical, and a similar SF2 of 54 +/- 1% was observed (P = 0.4). However, the individual colony size was significantly increased in all cultures incubated with FGF7 compared to those incubated without FGF7. The number of extremely large colonies (> or =2 mm) was clearly higher (P < 0.0001) in cultures with FGF7. This was accompanied by a significant reduction in the diameter of individual cells from 29 microm in controls to 23 microm with FGF7. In conclusion, FGF7 does not affect the survival of keratinocytes after irradiation, but it does stimulate proliferation of surviving cells.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Slonina D,Hoinkis C,Dörr Wdoi
10.1667/0033-7587(2001)156[0761:eokgfo]2.0.co;2subject
Has Abstractpub_date
2001-12-01 00:00:00pages
761-6issue
6eissn
0033-7587issn
1938-5404journal_volume
156pub_type
杂志文章abstract::The linear no-threshold extrapolation from a dose-response relationship for ionizing radiation derived at higher doses to doses for which regulatory standards are proposed is being challenged by some scientists and defended by others. It appears that the risks associated with exposures to doses of interest are below t...
journal_title:Radiation research
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doi:
更新日期:1998-12-01 00:00:00
abstract::Single crystals of the co-crystalline complex of 1-methyluracil and 9-ethyladenine were X-irradiated and studied using EPR, ENDOR and FSE spectroscopic techniques at 10 K. All together seven radicals were identified, and experimental evidence for at least one more species, as well as for a very low population of radic...
journal_title:Radiation research
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更新日期:1998-02-01 00:00:00
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journal_title:Radiation research
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doi:10.1667/0033-7587(2001)155[0724:eotibc]2.0.co;2
更新日期:2001-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::The potential carcinogenic effects of internally deposited alpha-particle-emitting nuclides, notably plutonium, in the liver in humans are unknown but are of concern in relation to exposures from the nuclear industry. However, patients injected with the radiographic contrast medium Thorotrast are chronically exposed t...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::Ionizing radiation induces bistranded clustered damages--two or more abasic sites, oxidized bases and strand breaks on opposite DNA strands within a few helical turns. Since clusters are refractory to repair and are potential sources of double-strand breaks (DSBs), they are potentially lethal and mutagenic. Although i...
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更新日期:2006-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::We collected initial quantitative information on the effects of high-dose carbon (12C) ions compared to photons on vascular damage in anaplastic rat prostate tumors, with the goal of elucidating differences in response to high-LET radiation, using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Syngene...
journal_title:Radiation research
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doi:10.1667/RR15459.1
更新日期:2020-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Given the distinct characteristics of low-energy electrons (LEEs), particularly at energies less than 30 eV, they can be applied to a wide range of therapeutic modalities to improve cancer treatment. LEEs have been shown to efficiently produce complex molecular damage resulting in substantial cellular toxicities. Sinc...
journal_title:Radiation research
pub_type: 杂志文章,评审
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更新日期:2017-08-01 00:00:00
abstract::Peroxidation of unconjugated polyunsaturated fatty acids such as linolenic acid proceeds through a free radical chain mechanism and is accompanied by the formation of conjugated dienes such as hydroperoxides. In an investigation of radiation-induced oxidation of aqueous linolenate, we have measured two indexes of pero...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0201:eotfod]2.0.co;2
更新日期:2000-02-01 00:00:00
abstract::Polonium-210 is a naturally occurring radioactive element that decays by emitting an alpha particle. It is in the air we breathe and also a component of tobacco smoke. Polonium-210 is used as an anti-static device in printing presses and gained widespread notoriety in 2006 after the poisoning and subsequent death of a...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR13395.1
更新日期:2014-02-01 00:00:00
abstract::A standard Fricke dosimeter was used to measure the absorbed dose via the oxidation yields of Fe3+ ions in an aqueous environment induced by soft X rays within the "water window" spectral range. We also exploited the property of a neutral solution containing terephthalic acid as a tool for selective detection of OH ra...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR15520.1
更新日期:2020-04-01 00:00:00
abstract::Technological developments in radiation therapy and other cancer therapies have led to a progressive increase in five-year survival rates over the last few decades. Although acute effects have been largely minimized by both technical advances and medical interventions, late effects remain a concern. Indeed, the need t...
journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/rr2530.1
更新日期:2012-04-01 00:00:00
abstract::Previous work from our laboratory suggested that neuronal chromatin structure may be altered immediately after exposure to ionizing radiation. In the present study, whole brains of 4-month-old male Fisher 344 rats were irradiated with a dose of 25 Gy. The kinetics of restoring the chromatin structure to its unirradiat...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-04-01 00:00:00
abstract::Radiation-induced complications in bone and cartilage are of increasing concern due to potential long-term effects in cancer survivors. Healthy articular cartilage may be exposed to radiation during either chondrosarcoma treatment or in-field radiotherapy of tumors located in close proximity to articulation. Cartilage...
journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/RR13928.1
更新日期:2015-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)158[0626:badeaf]2.0.co;2
更新日期:2002-11-01 00:00:00
abstract::Clinicians involved in conventional radiation therapy are very concerned about the dose-response relationships of normal tissues. Before proceeding to new clinical protocols, radiation biologists involved with conformal proton therapy believe it is necessary to quantify the dose response and tolerance of the organs an...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/r3016
更新日期:2003-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2157.1
更新日期:2010-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Cisplatin and binuclear rhodium (Rh2) carboxylates appear to potentiate radiation-induced killing of Salmonella typhimurium cells largely as a consequence of one-electron reduction that leads to an active radiolytic product. This conclusion is supported by results from experiments wherein the hydrated electron and the...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::Radiation sensitivity was measured by the micronucleus assay in T lymphocytes of the spleen and in fibroblasts derived from three inbred strains of mice, BALB/c, A/J and C3H/He. A linear correlation between the surviving fraction and the micronucleus frequency was obtained. There were clear differences in the radiosen...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Cells plated immediately after irradiation on nutrient agar (immediate plating) exhibit a lower survival than cells which are kept under nongrowth conditions before plating (delayed plating). The difference between the survival curves obtained after immediate plating and delayed plating is considered to exhibit the ce...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-08-01 00:00:00
abstract::The lineal energy distribution and several other dosimetric parameters were measured for the neutrons emitted from a replica of the Hiroshima bomb to determine their usefulness in biological experiments designed to estimate the effectiveness of actual Hiroshima neutrons. The "Little-Boy" replica (LBR) was constructed ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::To accurately quantify the number of single-strand breaks (SSBs) induced in plasmid DNA molecules after irradiation, a new type of assay methodology has been explored. The new method is based on the TUNEL (terminal deoxynucleotide transferase dUTP nick end-labeling) assay that was adopted for use under ELISA (enzyme-l...
journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
abstract::The potential for radiogenic neoplasia from charged-particle irradiation has been estimated using the Harderian gland of the mouse as a test system. Particles ranging in Z from Z = 1 (proton) to Z = 41 (niobium), in energy from 228 to 670A MeV, and in LET from 0.4 to 464 keV/microns were produced at the Lawrence Berke...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Wortmannin has been shown to be an efficient radiosensitizer. Since wortmannin is able to inhibit DNA-dependent protein kinase (DNA-PK) and double-strand break (DSB) rejoining, it is believed that its mechanism of radiation sensitization is through the inhibition of DNA-PK-mediated repair of DSBs. However, since wortm...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00