Abstract:
:It has been shown that several X-ray-sensitive Chinese hamster cell mutants defective in repair of DNA double-strand breaks (DSBs) are also impaired in the process of V(D)J recombination. The hamster mutants with this phenotype represent three distinct complementation groups, represented by the xrs series, XR-1 and V-3. The murine scid cell line also shows the same phenotype, and therefore we examined whether the scid mutant represents a new complementation group or belongs to one of the existing groups. Scid cells were fused with hamster cell mutants representing the three complementation groups. Hybrids between V-3 and scid cells were only partially complemented for X-ray sensitivity, whereas hybrids derived from fusions with the other mutants were resistant to X rays. These results suggest that V-3 and scid cells are defective in the same gene. To confirm this finding, a single human chromosome 8, which is known to carry the scid gene, was introduced into V-3 cells by microcell-mediated chromosome transfer. Nine hybrid clones derived from V-3 and carrying human chromosome 8 were obtained, and seven were found to be partially complemented for X-ray sensitivity. When human chromosome 8 was introduced into scid cells, seven of eight hybrid clones became resistant to X rays. The results indicate that the defective genes in V-3 and scid are both localized on human chromosome 8. This supports the results from the fusion analysis that V-3 and scid cells are defective in the same gene.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Zdzienicka MZ,Jongmans W,Oshimura M,Priestley A,Whitmore GF,Jeggo PAsubject
Has Abstractpub_date
1995-09-01 00:00:00pages
238-44issue
3eissn
0033-7587issn
1938-5404journal_volume
143pub_type
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1987-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0840:soamoc]2.0.co;2
更新日期:2000-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::Microwave radiation produces hyperthermia. The mammalian thermoregulatory system defends against changes in temperature by mobilizing diverse control mechanisms. Neurotransmitters play a major role in eliciting thermoregulatory responses. The involvement of adrenergic and muscarinic cholinergic receptors was investiga...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)156[0118:bdbaap]2.0.co;2
更新日期:2001-07-01 00:00:00
abstract::The increasing use of mobile phones by children and teenagers has raised concerns about their safety. Addressing such concerns is difficult, because no data are available on possible effects from long-term exposure to radiofrequency (RF) fields during the development of the nervous system. Possible morphological and f...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1002.1
更新日期:2007-10-01 00:00:00
abstract::Dose-spread kernels can be used to calculate the dose distribution in a photon beam by convolving the kernel with the primary fluence distribution. The theoretical relationships between various types and components of dose-spread kernels relative to photon attenuation coefficients are explored. These relations can be ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract::To compare the genotoxic effects of high-LET ionizing radiation to those of low-LET radiation, we investigated the responses of human lymphoblastoid cells to DNA damage TK6 after treatment with either low-LET X rays or high-LET iron ions (1000 keV/microm). A highly localized distribution of gammaH2AX/RAD51 foci was ob...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)158[0678:dccpih]2.0.co;2
更新日期:2002-12-01 00:00:00
abstract::The physical and biological basis of our model to calculate the biological effects of charged particles, termed the local effect model (LEM), has recently been questioned in a commentary by R. Katz. Major objections were related to the definition of the target size and the use of the term cross section. Here we show t...
journal_title:Radiation research
pub_type: 评论,杂志文章
doi:10.1667/rr3174
更新日期:2004-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::We studied the spatial and temporal distributions of foci of the phosphorylated form of the histone protein H2AX (gamma-H2AX), which is known to be activated by double-strand breaks after irradiation of human fibroblast cells with high-energy silicon (54 keV/microm) and iron (176 keV/microm) ions. Here we present data...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3431.1
更新日期:2005-10-01 00:00:00
abstract::Previous surveys of radiation therapy among the Life Span Study (LSS) population at the Radiation Effects Research Foundation (RERF) revealed that 1,670 (1.4%) of the LSS participants received radiation treatments before 1984. The data on therapeutic radiation doses are indispensable for studying the relationship betw...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)155[0785:odfrti]2.0.co;2
更新日期:2001-06-01 00:00:00
abstract::The efficiency of boron neutron capture therapy (BNCT) for malignant gliomas depends on the selective and absolute accumulation of (10)B atoms in tumor tissues. Only two boron compounds, BPA and BSH, currently can be used clinically. However, the detailed distributions of these compounds have not been determined. Here...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0501.1
更新日期:2007-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14574.1
更新日期:2017-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR3373.1
更新日期:2013-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::It is well established that high-LET radiations efficiently induce chromosome aberrations. However, data on the effect of protons on telomere maintenance, as involved in genomic stability, are scarce and contradictory. Here we demonstrate that high-LET protons induce telomere lengthening in human primary fibroblasts a...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2127.1
更新日期:2010-11-01 00:00:00
abstract::The effect of hypoxia on the induction of and recovery from damage by radiation alone and in combination with heat has been investigated using plateau-phase Chinese hamster ovary (CHO) cells. Postirradiation hypoxia reduced the potentially lethal damage recovery (PLDR) in cells irradiated under an euoxic state and com...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::The modification of methylglyoxal bis(guanylhydrazone) (MGBG) by 42 degrees C hyperthermia-and/or radiation-induced cell killing was examined in Chinese hamster V-79 cells. At concentrations of more than 10 microM, cell survival decreased exponentially with increased MGBG exposure times. Cell lethality of MGBG (10 mic...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::Exposure of humans and rodents to radiofrequency (RF) cell phone fields has been reported to alter a number of stress- related parameters. To study this potential relationship in more detail, tube-restrained immobilized Fischer 344 rats were exposed in the near field in a dose-dependent manner to pulse-modulated (11 p...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)155[0584:eoiace]2.0.co;2
更新日期:2001-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)154[0326:todiiq]2.0.co;2
更新日期:2000-09-01 00:00:00
abstract::New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours following irradiation, oxid...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1968-11-01 00:00:00