Abstract：
:We investigated delayed reproductive death and chromosome bridge formation in mitotic cells in colonies of normal human embryonic cells which survived exposure to 6 Gy of X rays. Eighteen primary clones each derived from control and irradiated cultures were isolated and secondary colonies were grown up. Six of the primary clones surviving the irradiation showed significantly lower cloning efficiencies than the control clones (P < 0.001), while the rest of the surviving clones showed a cloning efficiency similar to those of the control clones. The average cloning efficiency of control and surviving clones was 16.4 and 7.2%, respectively. Reduced cloning efficiencies were also observed in the tertiary colony formation, indicating that the persistent decrease in cloning efficiency was inherited over 40 generations after X irradiation in normal human cells. Five of nine primary clones surviving the irradiation also frequently contained multiple giant cells in the colonies, while this was a rare event in the progeny of control clones. Furthermore, we analyzed in situ chromosome segregation in anaphase cells appearing during the formation of the secondary colonies. A significantly higher frequency of chromosome bridge formation was found in four of the surviving clones which received 6 Gy (P < 0.001), and the frequency of micronuclei was also increased in the surviving clones. These results provide evidence that genetic instability is induced in normal human embryonic cells by low-LET radiation, and that misrejoining of the broken chromosome ends is increased in the progeny of cells surviving X irradiation.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Suzuki K,Takahara R,Kodama S,Watanabe Msubject
Has Abstractpub_date
1998-10-01 00:00:00pages
375-81issue
4eissn
0033-7587issn
1938-5404journal_volume
150pub_type
杂志文章abstract：:Currently, there is a serious absence of pharmaceutically attractive small molecules that mitigate the lethal effects of an accidental or intentional public exposure to toxic doses of ionizing radiation. Moreover, cellular systems that emulate the radiobiologically relevant cell populations and that are suitable for h...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2810.1
更新日期：2012-09-01 00:00:00
abstract：:A statistical technique is given which can be used to estimate the parameters of the two-component model for cell survival from quantal response multifraction data. The method is a nonlinear logistic regression and relies on a mild assumption relating the probability of death to cell survival level. The method is demo...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:While lifespan studies provide basic information for estimating the risk of ionizing radiation, findings on the effect of low-dose/low-dose-rate irradiation on the lifespan of mammals are controversial. Here we evaluate the effect of continuous exposure to low-dose-rate γ radiation on the lifespan of mice with acceler...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2977.1
更新日期：2013-06-01 00:00:00
abstract：:In the event of a large-scale radiation exposure, accurate and quick assessment of radiation dose received would be critical for triage and medical treatment of large numbers of potentially exposed individuals. Current methods of biodosimetry, such as the dicentric chromosome assay, are time consuming and require soph...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14655.1
更新日期：2017-06-01 00:00:00
abstract：:Phosphorylated ATM immunofluorescence staining was used to investigate the dose-response relationship for the number of DNA double-strand breaks (DSBs) induced in primary normal human fibroblasts irradiated with doses from 1.2 to 200 mGy. The induction of DSBs showed a supralinear dose-response relationship. Radiation...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1255.1
更新日期：2008-09-01 00:00:00
abstract：:The response of the testes of two strains of adult rats (Sprague-Dawley and Wistar) to graded single doses and split doses of 230 kVp X rays has been investigated. A marked difference was noted between the strains in the response of the clonogenic spermatogonia to irradiation, as measured histologically by the repopul...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:Given the distinct characteristics of low-energy electrons (LEEs), particularly at energies less than 30 eV, they can be applied to a wide range of therapeutic modalities to improve cancer treatment. LEEs have been shown to efficiently produce complex molecular damage resulting in substantial cellular toxicities. Sinc...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/RR14727.1
更新日期：2017-08-01 00:00:00
abstract：:If the limited life span of hematopoietic tissues in vitro is due to a finite proliferative capacity of individual stem cells, one might expect tissues of young donors to possess a greater proliferative capacity and to contain a larger population of primitive stem cells than those of older donors. To test this hypothe...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:Some cells have been reported to show greater resistance to drugs or radiation when growing with close intercellular contacts in spheroids or in solid tumors than when growing with few intercellular contacts in sparse cultures. In some cases this increased resistance reflects an increased capacity of cells in close co...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：:Radiation-induced cell death is thought to be caused by nuclear DNA damage that cannot be repaired. However, in this study we found that a delayed increase of mitochondrial reactive oxygen species (ROS) is responsible for some of the radiation-induced cell death in normal human fibroblast cells. We have previously rep...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13772.1
更新日期：2015-04-01 00:00:00
abstract：:Microwave radiation produces hyperthermia. The mammalian thermoregulatory system defends against changes in temperature by mobilizing diverse control mechanisms. Neurotransmitters play a major role in eliciting thermoregulatory responses. The involvement of adrenergic and muscarinic cholinergic receptors was investiga...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:To investigate whether exposure to atomic bomb radiation altered the prevalence of hepatitis C virus (HCV) infection or accelerated the progress toward chronic hepatitis after HCV infection, the seropositivity of antibody to hepatitis C virus (anti-HCV) was determined for 6,121 participants in the Adult Health Study o...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)154[0012:poahcv]2.0.co;2
更新日期：2000-07-01 00:00:00
abstract：:Radon is recognized as a public health concern for indoor exposure. Precise quantification derived from occupational exposure in miners is still needed for estimating the risk and the factors that modify the dependence on cumulated exposure. The present paper reports on relationship between radon exposure and lung can...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0848.1
更新日期：2008-02-01 00:00:00
abstract：:Molecular imaging is an evolving science that is concerned with the development of novel imaging probes and biomarkers that can be used to non-invasively image molecular and cellular processes. This special issue approaches molecular imaging in the context of radiation research, focusing on biomarkers and imaging meth...
journal_title：Radiation research
pub_type：
doi：10.1667/rr2959.1
更新日期：2012-04-01 00:00:00
abstract：:In a patient with Morbus Hodgkin, structural aberrations of the chromosome type in peripheral lymphocytes were analyzed during radiation therapy (accumulated target dose 44.6 Gy: 22 fractions of 1.8 Gy each and 2 fractions of 2.5 Gy each at the end of the therapy). The blood was sampled about 5 min after a fraction an...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:In previous studies, flavonoids, orientin and vicenin, that were isolated from the leaf extract of Ocimum sanctum, were found to protect mice against radiation injury. Several flavonoids are known to be good antioxidants. Therefore, the effect of orientin and vicenin on radiation-induced lipid peroxidation in vivo and...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)154[0455:rpbtof]2.0.co;2
更新日期：2000-10-01 00:00:00
abstract：:We showed previously that autosomal recessive determinants control the development of pulmonary fibrosis in mice during the early and late phases after irradiation. The extent of fibrosis was inversely correlated with the intrinsic lung activity of both plasminogen activator (PLA) and angiotensin-converting enzyme (AC...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:The radiation dose received by crew members in interplanetary space is influenced by the stage of the solar cycle. Using the recently developed models of the galactic cosmic radiation (GCR) environment and the energy-dependent radiation transport code, we have calculated the dose at 0 and 5 cm water depth; using a com...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:Radiation sensitivity was measured by the micronucleus assay in T lymphocytes of the spleen and in fibroblasts derived from three inbred strains of mice, BALB/c, A/J and C3H/He. A linear correlation between the surviving fraction and the micronucleus frequency was obtained. There were clear differences in the radiosen...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:Gemcitabine (dFdCyd) shows broad antitumor activity in solid tumors in chemotherapeutic regimens or when combined with ionizing radiation (radiosensitization). While it is known that mismatches in DNA are necessary for dFdCyd radiosensitization, the critical event resulting in radiosensitization has not been identifie...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14443.1
更新日期：2016-11-01 00:00:00
abstract：:The effectiveness of 5-fluorouracil (5-FU), FT-207 and FT-207 + uracil in combination with two repetitions of 43 degrees C hyperthermia in the treatment of the Meth-A-Fibrosarcoma and Sarcoma-180 was examined in vivo in BALB/c mice. The antitumor effect was evaluated in terms of inhibition of tumor growth by measuring...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:Mice were irradiated with 1 Gy of fission neutrons. At intervals up to 15 days after irradiation undifferentiated spermatogonia were counted in whole mounts of seminiferous tubules in up to eight stages of the epithelial cycle. From Day 6 onward lower numbers of spermatogonia were found in the areas which were in stag...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:Our goal was to determine whether esophageal progenitor cells could be isolated from adult mouse esophagus or bone marrow and shown to home to and proliferate in the irradiated esophagus of recipient mice. Esophageal progenitor cells were isolated from adult male C3H/HeNsd or C57BL/6J green fluorescent protein (GFP(+)...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3224
更新日期：2004-09-01 00:00:00
abstract：:In a scenario involving a nuclear detonation during war or a terrorist attack, acute radiation exposure combined with thermal and blast effects results in severe skin injury. Although the cutaneous injury in such a scenario may not be lethal, it may lead to inflammation, delayed wound healing and loss of the skin barr...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14884.1
更新日期：2018-04-01 00:00:00
abstract：:Soil microorganisms in general and biocontrol agents in particular are very sensitive to UV light. The packaging of biocontrol microorganisms into cellular solids has been developed as a means of reducing loss caused by exposure to environmental UV radiation. The bacterial and fungal biocontrol agents Pantoea agglomer...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3027
更新日期：2003-08-01 00:00:00
abstract：:We recently reported that daily dietary supplementation with 100 µg selenium (a dose exceeding a rat's nutritional requirement by about 33-fold) initiated immediately after total-body irradiation (TBI) and maintained for 21 weeks mitigates radiation nephropathy in a rat model as indicated by blood urea nitrogen (BUN) ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2456.1
更新日期：2011-09-01 00:00:00
abstract：:Tritium that is bound to organic molecules is of special risk for living systems, in particular when such molecules are components of the cell nucleus. Therefore, [3H]thymidine and [3H]arginine were studied for radiotoxicity in early mammalian embryo development. Starting with the two-cell stage, mouse embryos were in...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:The non-human primate has been a useful model for studies of human acute radiation syndrome (ARS). However, to date structural changes in various parts of the intestine after total body irradiation (TBI) have not been systematically studied in this model. Here we report on our current study of TBI-induced intestinal s...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14191.1
更新日期：2015-11-01 00:00:00
abstract：:The relationship between the intracellular glutathione (GSH) concentration and the aerobic radiation response was studied in Chinese hamster ovary cells. Various degrees of GSH depletion were produced by exposure to buthionine sulfoximine (BSO) and/or diethyl maleate (DEM). Diethyl maleate did not act as a classical r...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:The analysis of mitotic indices (MI) and chromosome breakages in metaphases of 50-hr lymphocyte cultures exposed to the calcium or zinc chelates of diethylenetriamine pentaacetic acid (DTPA) demonstrated: (1) an 80% reduction in MI in cultures from three women but no reduction in those from two men after in vitro expo...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
