Abstract:
:In previous studies, flavonoids, orientin and vicenin, that were isolated from the leaf extract of Ocimum sanctum, were found to protect mice against radiation injury. Several flavonoids are known to be good antioxidants. Therefore, the effect of orientin and vicenin on radiation-induced lipid peroxidation in vivo and their antioxidant activity in vitro were studied. Adult mice were injected intraperitoneally with 50 microgram/kg of orientin or vicenin and exposed whole-body to 3 Gy of gamma radiation. Lipid peroxidation was measured in the liver 15 min to 8 h postirradiation. The antioxidant activity of orientin/vicenin (10-500 microM) was studied by measuring inhibition of hydroxyl radicals generated by the Fenton reaction (Fe(3+)-EDTA-ascorbic acid-H(2)O(2)) in vitro. The compounds were also tested for possible pro-oxidant and iron chelation activities at the above concentrations in the in vitro system. Orientin and vicenin provided almost equal protection against radiation-induced lipid peroxidation in mouse liver. Both compounds showed a significantly greater free radical-inhibiting activity in vitro than DMSO. Neither orientin nor vicenin showed any pro-oxidant activity at the concentrations tested. Both compounds inhibited free radical formation in the absence of EDTA. Free radical scavenging appears to be a likely mechanism of radiation protection by these flavonoids.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Uma Devi P,Ganasoundari A,Vrinda B,Srinivasan KK,Unnikrishnan MKdoi
10.1667/0033-7587(2000)154[0455:rpbtof]2.0.co;2subject
Has Abstractpub_date
2000-10-01 00:00:00pages
455-60issue
4eissn
0033-7587issn
1938-5404journal_volume
154pub_type
杂志文章abstract::We tested the possibility that hyperthermia kills HA-1 cells in a manner analogous to growth factor deprivation. HA-1 cells were inactivated by serum starvation when incubated in Eagle's MEM at a density of 40 cells/cm2 or less. Cells became resistant to the absence of serum when the cell density was greater than 400 ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-04-01 00:00:00
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journal_title:Radiation research
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更新日期:1998-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3353
更新日期:2005-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
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doi:
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pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
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