Abstract：
:A yeast-based frameshift/stop codon assay for examining ATM (ataxia telangiectasia mutated) mutations was established. Each of six fragments of a PCR-amplified coding sequence for ATM is inserted in frame by homologous recombination into a yeast URA3 fusion protein gene, and the transformants are assayed for growth in the absence of uracil. The usefulness of this assay was verified in a panel of cell lines derived from individuals with homozygous and heterozygous ATM mutations. The assay was also shown to distinguish between specimens with wild-type alleles and those with truncating mutations: a frameshift mutation or an inserted stop codon. Using this assay M059J cells, which fail to express the catalytic subunit of DNA-dependent protein kinase (PRKDC, also known as DNA-PKcs) and are hypersensitive to ionizing radiation, were found to express two different aberrant ATM transcripts: one characterized by 4776 del 133, which corresponds to the deletion of exon 33, and the other by 4909 ins 116. Subsequent analysis of the intron sequences revealed that 4909 ins 116 is comprised of a nucleotide sequence corresponding to 84013-84128 in intron 33 with a cryptic splice site. Thus the radiosensitive phenotype of M059J cells appears to be due to a defect in PRKDC and a truncating ATM mutation.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Tsuchida R,Yamada T,Takagi M,Shimada A,Ishioka C,Katsuki Y,Igarashi T,Chessa L,Delia D,Teraoka H,Mizutani Sdoi
10.1667/0033-7587(2002)158[0195:doagmi]2.0.co;2subject
Has Abstractpub_date
2002-08-01 00:00:00pages
195-201issue
2eissn
0033-7587issn
1938-5404journal_volume
158pub_type
杂志文章abstract：:Mortality data for B6CF1 mice exposed to 60Co gamma rays for the duration of life were used to make quantitative predictions of age-specific mortality observed in comparably exposed beagles. Simple Kaplan-Meier survival curves for the beagles and their 95% confidence intervals were computed for each dose-rate group ob...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:Chinese hamster ovary (CHO) cells were exposed to various concentrations of diethylmaleate (DEM) during a 42 degrees C incubation to determine if glutathione (GSH) compartmentalization was a factor in modification of thermal sensitivity. Cytoplasmic and mitochondrial GSH were isolated from CHO cells immediately after ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:Well-defined protocols and quality management standards are indispensable for biological dosimetry laboratories. Participation in periodic proficiency testing by interlaboratory comparisons is also required. This harmonization is essential if a cooperative network is used to respond to a mass casualty event. Here we p...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2425.1
更新日期：2011-05-01 00:00:00
abstract：:This work provides a description of a new interaction, cross-section-based model for radiation-induced cellular inactivation, sublethal damage, DNA repair and cell survival, with the ability to more accurately elucidate different radiation-response phenomena. The principal goal of this work is to describe the damage-i...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RADE-20-00052.1
更新日期：2020-09-16 00:00:00
abstract：:Electron microbeam experiments are planned or under way to explore in part the question regarding whether the bystander effect is a general phenomenon or is restricted to high-LET radiation. Since low-LET radiations scatter more readily compared to high-LET radiations, identifying bystander cells and assessing the pot...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0089:moakem]2.0.co;2
更新日期：2001-01-01 00:00:00
abstract：:Wortmannin has been shown to be an efficient radiosensitizer. Since wortmannin is able to inhibit DNA-dependent protein kinase (DNA-PK) and double-strand break (DSB) rejoining, it is believed that its mechanism of radiation sensitization is through the inhibition of DNA-PK-mediated repair of DSBs. However, since wortm...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:In a recent study (Int. J. Radiat. Oncol. Biol. Phys. 36, 635-639, 1996), 1H nuclear magnetic resonance (NMR) spectroscopy was used to demonstrate significant decreases in lactate levels after gamma irradiation of radiosensitive RIF-1 tumors in vitro. For comparison, we have examined the effects of gamma radiation on ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:A statistical technique is given which can be used to estimate the parameters of the two-component model for cell survival from quantal response multifraction data. The method is a nonlinear logistic regression and relies on a mild assumption relating the probability of death to cell survival level. The method is demo...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:Wen, B., Urano, M., Humm, J. L., Seshan, V. E., Li, G. C. and Ling, C. C. Comparison of Helzel and OxyLite Systems in the Measurements of Tumor Partial Oxygen Pressure (pO(2)). Radiat. Res. 168, 67-75 (2008). It has been demonstrated in both experimental and human malignancies that hypoxic tumor cells are linked with ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0888.1
更新日期：2008-01-01 00:00:00
abstract：:Chomentowski, M., Kellerer, A. M. and Pierce, D. A. Radiation Dose Dependences in the Atomic Bomb Survivor Cancer Mortality Data: A Model-Free Visualization. The standard approach to obtaining nominal risk coefficients for radiation-related cancer involves fitting linear or linear-quadratic dose-response functions. Th...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)153[0289:rddita]2.0.co;2
更新日期：2000-03-01 00:00:00
abstract：:X rays have been shown to induce two forms of potentially lethal damage (PLD), "fast-repairing" PLD and "slowly repairing" PLD, whose repair is completed in 1 h and 4-6 h, respectively. In this study three modes of treatment with hypertonic solutions containing different NaCl concentrations for different durations (0....
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Single crystals of anhydrous inosine were studied subsequent to exposure to high and low doses of X radiation at 10 K using K-band, EPR, ENDOR, and field-swept-ENDOR (FSE) techniques. Immediately following high radiation doses at 10 K at least eight different radicals, RI-RVIII, were observed. All radicals, except for...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:Statistical analysis of Monte Carlo simulations of positive ion track structure is presented for 1 MeV protons passing outside an absorbing region of interest. Energy deposition and production of ionization within the region occur only by delta-ray transport from the ion's path. Depositions in spherical absorber regio...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:Radiation-induced free radical damage in solid-state DNA arises from direct-type damage mechanisms. In the present study, free radical yields in film and lyophilized Na DNA model systems are compared. The free radical yields in lyophilized samples are significantly greater than those in films. Since DNA conformation c...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:We showed previously that autosomal recessive determinants control the development of pulmonary fibrosis in mice during the early and late phases after irradiation. The extent of fibrosis was inversely correlated with the intrinsic lung activity of both plasminogen activator (PLA) and angiotensin-converting enzyme (AC...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Evidence is presented for the formation of products in irradiated dinucleoside monophosphates in which both bases are damaged. The dinucleoside monophosphates d(GpT), d(GpC), d(TpG) and d(CpG) were X-irradiated in oxygenated aqueous solution. Product identification was by NMR spectroscopy. In products containing doubl...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Incorporation of bromodeoxyuridine (BrdU) into DNA in both Chinese hamster ovary (CHO) cells and human melanoma (U1) cells reduced the rate of DNA migration in transverse alternating-field electrophoresis (TAFE) agarose gel optimized for separating molecules larger than 2 Mb. This "BrdU migration effect" was independe...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:The present study was undertaken to standardize a dichlorofluorescein (DCF) assay for measurement of radiation-induced oxidation of dichlorofluorescin (DCFH) substrate in MCF-10 cells. This assay was highly sensitive and capable of detecting increased DCFH oxidation in the cells exposed to gamma radiation at doses as ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3299
更新日期：2005-02-01 00:00:00
abstract：:Previous studies identified radiation therapy as a key modifier of basal cell carcinoma (BCC) risk in survivors of hematopoietic cell transplantation (HCT). In the present analysis, risk of BCC was analyzed in relation to age at transplant, attained age, race, total-body irradiation (TBI), and radiation fractionation ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1469.1
更新日期：2009-02-01 00:00:00
abstract：:Synchronous Chinese hamster ovary cells were treated in G1 or S phase with cycloheximide or procaine hydrochloride before and during heating at 43 degrees C. Cycloheximide and procaine apparently act by different mechanisms, with cycloheximide inhibiting protein synthesis and procaine hydrochloride supposedly affectin...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:The influence of Trp53 on the radiation-induced elevation of T-cell receptor (TCR) variant fractions was examined in splenic T lymphocytes of Trp53-proficient and -deficient mice. Wild-type Trp53+/+, heterozygous Trp53+/- and null Trp53-/- mice were exposed to 3 Gy of X rays at 8 weeks of age. The fraction of TCR-defe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3583.1
更新日期：2006-07-01 00:00:00
abstract：:Tissue-incorporated radionuclides impart radiation energy over extended periods of time depending on their effective half-lives. The capacity of vitamin A dissolved in soybean oil to protect against the biological effects caused by internal radionuclides is investigated. The radiochemicals examined are DNA-binding 125...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:A method was used to detect chromosome inversions as apparent or false sister chromatid exchanges (SCEs) in the first mitosis after gamma irradiation of human G0 cells. Dose-response relationships for small inversions have not been measured and reported previously, but it has been assumed that these are induced with a...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:We have previously used NotI as the marker enzyme (recognizing GCGGCCGC) in a genome scanning approach for detection of mutations induced in mouse spermatogonia and estimated the mutation induction rate as about 0.7 x 10(-5) per locus per Gy. To see whether different parts of the genome have different sensitivities fo...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0926.1
更新日期：2007-08-01 00:00:00
abstract：:In the event of a radiation accident or attack, it will be imperative to quickly assess the amount of radiation exposure to accurately triage victims for appropriate care. RNA-based radiation dosimetry assays offer the potential to rapidly screen thousands of individuals in an efficient and cost-effective manner. Howe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr1977.1
更新日期：2011-02-01 00:00:00
abstract：:Angiogenesis defines the many steps involved in the growth and migration of endothelial cell-derived blood vessels. This process is necessary for the growth and metastasis of tumors, and considerable effort is being expended to find inhibitors of tumor angiogenesis. This usually involves screening of potential anti-an...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0171:rdaion]2.0.co;2
更新日期：2001-01-01 00:00:00
abstract：:Alpha-particle irradiation of cells damages not only the irradiated cells but also nontargeted bystander cells. It has been proposed that the bystander effect is caused by oxidants and free radicals generated by the radiation. Recent studies have shown that α(1)-microglobulin protects against cell damage caused by oxi...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2213.1
更新日期：2010-11-01 00:00:00
abstract：:The exposure of peripheral blood mononuclear cells (PBMCs) was performed in a 10-T static magnetic field. Without lymphocyte stimulation, there were no significant differences in the viability of the exposed and unexposed CD4(+) T cells, CD8(+) T cells, B cells, and natural killer (NK) cells. The expression of Th1 typ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2003)159[0775:eotsmf]2.0.co;2
更新日期：2003-06-01 00:00:00
abstract：:A computer model that simulates the killing of exponentially growing cells by low-dose-rate radiation is described. The model incorporates cell killing by single-hit damage and double-hit (sublethal) damage, as well as repair of sublethal damage, delay of cell cycle progression, blockage and increased sensitivity of c...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:The filter elution technique using nondenaturing conditions is widely used to assay DNA double-strand break (DSB) induction and repair. It has been reported that in the measurement of strand breaks higher rates of elution and of initial rejoining are obtained at pH 9.6 compared to pH 7.2. In the present experiments ne...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
