Abstract:
:We have previously used NotI as the marker enzyme (recognizing GCGGCCGC) in a genome scanning approach for detection of mutations induced in mouse spermatogonia and estimated the mutation induction rate as about 0.7 x 10(-5) per locus per Gy. To see whether different parts of the genome have different sensitivities for mutation induction, we used AflII (recognizing CTTAAG) as the marker enzyme in the present study. After the screening of 1,120 spots in each mouse offspring, we found five mutations among 92,655 spots from the unirradiated paternal genome, five mutations among 218,411 spots from the unirradiated maternal genome, and 13 mutations among 92,789 spots from 5 Gy-exposed paternal genome. Among the 23 mutations, 11 involved mouse satellite DNA sequences (AT-rich), and the remaining 12 mutations also involved AT-rich but non-satellite sequences. Both types of sequences were found as multiple, similar-sequence blocks in the genome. Counting each member of cluster mutations separately and excluding results on one hypermutable spot, the spontaneous mutation rates were estimated as 3.2 (+/- 1.9) x 10(-5) and 2.3 (+/- 1.0) x 10(-5) per locus per generation in the male and female genomes, respectively, and the mutation induction rate as 1.1 (+/- 1.2) x 10(-5) per locus per Gy. The induction rate would be reduced to 0.9 x 10(-5) per locus per Gy if satellite sequence mutations were excluded from this analysis. The results indicate that mutation induction rates do not largely differ between GC-rich and AT-rich regions: 1 x 10(-5) per locus per Gy or less, which is close to 1.08 x 10(-5) per locus per Gy, the current estimate for the mean mutation induction rate in mice.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Asakawa J,Nakamura N,Katayama H,Cullings HMdoi
10.1667/RR0926.1subject
Has Abstractpub_date
2007-08-01 00:00:00pages
158-67issue
2eissn
0033-7587issn
1938-5404pii
RR0926journal_volume
168pub_type
杂志文章abstract::Irradiation from internally deposited radionuclides induces malignant tumors. Ingested long-lived radionuclides accumulate in specific organs that are irradiated throughout life. To elucidate why the development of malignant tumors requires long-term internal exposure, of the order of decades, despite the fact that ir...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1492.1
更新日期:2009-04-01 00:00:00
abstract::A dose of heat which renders 98% of a population of Chinese hamster ovary cells reproductively dead has no significant effect on their Na+, K+, or Mg2+ content by 28 h postheat. In contrast, the cellular Ca2+ content increases in a dose-dependent manner as observed at 22 h after heating for 15-35 min at 45 degrees C. ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-02-01 00:00:00
abstract::The flow cytometry mutation assay is based on detecting mutations in the CD59 gene on human chromosome 11 in CHO A(L) cells with flow cytometry, but the kinetics of mutant expression and the histogram region for mutant selection have not been studied in detail. CHO A(L) cells were analyzed by flow cytometry for CD59 e...
journal_title:Radiation research
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doi:10.1667/RR1276.1
更新日期:2008-11-01 00:00:00
abstract::Existence of a substantial fraction of replicon initiation events refractory to the effects of X irradiation in Chinese hamster cells has been reported by several laboratories. The work reported here examined whether this apparently refractive fraction resulted from a delayed inhibition of initiation events. Data obta...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Braun, R. D., Lanzen, J. L., Turnage, J. A., Rosner, G. and Dewhirst, M. W. Effects of the Interaction between Carbogen and Nicotinamide on R3230 Ac Tumor Blood Flow in Fischer 344 Rats. Radiat. Res. 155, 724-733 (2001). The purpose of this study was to determine whether there are interactions between carbogen breathi...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)155[0724:eotibc]2.0.co;2
更新日期:2001-05-01 00:00:00
abstract::Bcl-X(S) is a pro-apoptosis member of the Bcl2 family that has been shown to induce cell death and enhance chemosensitivity. We have investigated the effect of Bcl-X(S) overexpression on radiation sensitivity. Using a tetracycline-repressible system, we found that removal of tetracycline for 16 h induced Bcl-X(S) and ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3169
更新日期:2004-05-01 00:00:00
abstract::A yeast-based frameshift/stop codon assay for examining ATM (ataxia telangiectasia mutated) mutations was established. Each of six fragments of a PCR-amplified coding sequence for ATM is inserted in frame by homologous recombination into a yeast URA3 fusion protein gene, and the transformants are assayed for growth in...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)158[0195:doagmi]2.0.co;2
更新日期:2002-08-01 00:00:00
abstract::Aberrant expression of microRNAs (miRNAs) is frequently associated with a variety of cancers, including breast cancer. We and others have demonstrated that radiation-induced rat mammary cancer exhibits a characteristic gene expression profile and a random increase in aberrant DNA copy number; however, the role of aber...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2927.1
更新日期:2013-02-01 00:00:00
abstract::The presence of low levels of oxygen may have profound effects on the cytotoxic activity of radiation, radiosensitizers, and bioreductive alkylating agents. As others have shown, low oxygen tensions may significantly alter rates of cellular and chemical oxygen consumption. When experiments are performed at very low ox...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
abstract::The effect of hypoxia on the induction of and recovery from damage by radiation alone and in combination with heat has been investigated using plateau-phase Chinese hamster ovary (CHO) cells. Postirradiation hypoxia reduced the potentially lethal damage recovery (PLDR) in cells irradiated under an euoxic state and com...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::Molecular oxygen has long been recognized as a powerful radiosensitizer that enhances the cell-killing efficiency of ionizing radiation. Radiosensitization by oxygen occurs at very low concentrations with the half-maximum radiosensitization at approximately 3 mmHg. However, robust hypoxia-induced signal transduction c...
journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/RR13959.1
更新日期:2015-05-01 00:00:00
abstract::Synchrotron microbeam radiation treatment (MRT) is a preclinical radiotherapy technique with considerable clinical promise, although some of the underlying radiobiology of MRT is still not well understood. In recently reported studies, it has been suggested that MRT elicits a different tumor immune profile compared to...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14115.1
更新日期:2016-01-01 00:00:00
abstract::This report compares the deterministic effects from an alpha-particle-emitting radionuclide, (239)PuO(2), and a beta-particle emitter, (144)Ce in fused aluminosilicate particles (FAP). The studies were conducted in beagle dogs of both genders exposed by inhalation to aerosols of the radionuclides. The initial lung bur...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Evaluation of the probability of biological phenomena per cell by colony formation, requires knowledge not only of the number of cells at risk but also of their microdistribution. In the present study, the influence of cellular multiplicity (number of cells per potential colony-forming unit) on the determination of ra...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Experiments were performed to test whether significant amounts of pyrimidine dimers are produced in cultured cells of tobacco (Nicotiana tabacum L. var. Xanthi) and of Haplopappus gracilis by ultraviolet light in the biological dose range and whether either or both dark and light repair systems exist in these cells. T...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1968-11-01 00:00:00
abstract::The efficiency of boron neutron capture therapy (BNCT) for malignant gliomas depends on the selective and absolute accumulation of (10)B atoms in tumor tissues. Only two boron compounds, BPA and BSH, currently can be used clinically. However, the detailed distributions of these compounds have not been determined. Here...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0501.1
更新日期:2007-01-01 00:00:00
abstract::Clustered damage sites other than double-strand breaks (DSBs) have the potential to contribute to deleterious effects of ionizing radiation, such as cell killing and mutagenesis. In the companion article (Semenenko et al., Radiat. Res. 164, 180-193, 2005), a general Monte Carlo framework to simulate key steps in the b...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3414
更新日期:2005-08-01 00:00:00
abstract::In this study, we investigated the accuracy of using off-line bioluminescence imaging (BLI) and tomography (BLT) to guide irradiation of small soft tissue targets on a small animal radiation research platform (SARRP) with on-board cone beam CT (CBCT) capability. A small glass bulb containing BL cells was implanted as ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2999.1
更新日期:2013-02-22 00:00:00
abstract::Neurons are essential components of neural circuits and provide brain function organization. We previously reported that X irradiation induces apoptosis in immature neurons. To the best of our knowledge, there have been few reports investigating the effects of X irradiation on mature neurons. We analyzed the effects o...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR3098.1
更新日期:2013-06-01 00:00:00
abstract::The presence of oxygen-deficient tumor cells is a critical issue in cancer therapy. To identify tumor hypoxia, tissue partial oxygen pressure (pO2) can be measured directly. The OxyLite system allows determination of pO2 in tumors and permits continuous measurements of pO2 at a fixed point. In this study, this system ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR3602.1
更新日期:2006-09-01 00:00:00
abstract::V79 Chinese hamster cells were irradiated in the presence of different amounts of boric acid with thermal neutrons at the Medical Research Reactor at Brookhaven National Laboratory. From the linear dose-survival curves observed, a D0 value of 66 rad for the 10B(n, alpha) 7Li neutron capture reaction was obtained. No d...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Mixed radiation fields comprise the most common form of radiation exposure. Given their relevance in radiation protection, cancer radiotherapy and space research, accurate predictions of the corresponding radiation effects are essential. The local effect model (LEM) allows the prediction of cell survival after ion irr...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR15456.1
更新日期:2020-02-01 00:00:00
abstract::Plutonium is considered to be a carcinogen because it emits alpha particles that may result in the irradiation of stem cell population. In the present study we show that plutonium can also catalyze reactions that induce hydroxyl radicals in the absence of significant alpha-particle irradiation. Using the low specific ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Pulse radiolysis and 60Co gamma radiolysis were used to study the effects of ionizing radiations on the activity of glutamate dehydrogenase. Hydroxyl radicals are considerably more effective than hydrated electrons in causing loss of enzymatic activity. Evidence is also presented that the free radical anions (SCN)-.2,...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Synchronous Chinese hamster ovary cells were treated in G1 or S phase with cycloheximide or procaine hydrochloride before and during heating at 43 degrees C. Cycloheximide and procaine apparently act by different mechanisms, with cycloheximide inhibiting protein synthesis and procaine hydrochloride supposedly affectin...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::Telomeres cap the ends of chromosomes, preventing end-to-end fusions and subsequent chromosome instability. Here we used a telomerase knockout model to investigate whether telomerase participates in the processes of DNA break repair by de novo synthesis of telomere repeats at broken chromosome ends (chromosome healing...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3203
更新日期:2004-08-01 00:00:00
abstract::The effects of reoxygenation on repair of potentially lethal radiation damage were investigated using MG-63 human osteosarcoma cells in vitro. When exponentially growing MG-63 cells were cultured under hypoxic conditions for 24 h, cells stopped growing and remained at a low density. The hypoxic cells were then reoxyge...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Eisenia fetida were exposed continuously to (60)Co gamma radiation during two generations (F(0) and F(1)). Adult F(0) reproduction capacity (i.e., number of cocoons produced, hatchability and number of F(1) hatchlings) in controls and at five dose rates (0.18, 1.7, 4, 11 and 43 mGy/h) was measured over a 13-week expos...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1012.1
更新日期:2007-11-01 00:00:00
abstract::A historical cohort mortality study was conducted among 28,008 white male employees who had worked for at least 1 month in Oak Ridge, Tennessee, during World War II. The workers were employed at two plants that were producing enriched uranium and a research and development laboratory. Vital status was ascertained thro...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Human cancers have multiple alterations in cell signaling pathways that promote resistance to cytotoxic therapy such as X rays. Parthenolide is a sesquiterpene lactone that has been shown to inhibit several pro-survival cell signaling pathways, induce apoptosis, and enhance chemotherapy-induced cell killing. We invest...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1128.1
更新日期:2007-12-01 00:00:00