Abstract:
:X rays have been shown to induce two forms of potentially lethal damage (PLD), "fast-repairing" PLD and "slowly repairing" PLD, whose repair is completed in 1 h and 4-6 h, respectively. In this study three modes of treatment with hypertonic solutions containing different NaCl concentrations for different durations (0.5 M for 30 min, 0.225 M for 4 h, 0.16 M for 16 h) were examined to determine which form of PLD is expressed under each condition. These three modes of treatment enhanced the cell-killing action of X rays on actively growing V79 cells due to fixation of PLD. The kinetics of recovery from PLD was assessed by delayed treatments with hypertonic solutions. Cells exposed to one of the three treatments (see above) had completed recovery times of 1, 4 and 8 h, respectively, suggesting the possibility that these three modes of treatment cause the expression of different forms of PLD. As has been reported, treatment with 0.5 M NaCl for 30 min expressed fast-repairing PLD. The independence of the PLD expressed by 0.225 M NaCl for 4 h from fast-repairing PLD expressed after 0.5 M NaCl for 30 min was shown by combined treatment with the two modes, which reduced survival to the level that would be reached if the two modes acted independently. The data on the recovery time and on the inhibition by 0.225 M NaCl of recovery from slowly repairing PLD in plateau-phase cells indicated that the PLD expressed after 0.225 M NaCl for 4 h may be related to slowly repairing PLD. The combined treatment of 0.16 M NaCl for 16 h with 0.225 M NaCl for 4 h indicated independent action, albeit incomplete, of the PLD expressed after 0.16 M NaCl for 16 h from slowly repairing PLD. We propose for the first time that "very slowly repairing" PLD is expressed after 0.16 M NaCl for 16 h in exponentially growing cells and that therefore three forms of PLD are expressed by hypertonic treatments after X irradiation.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Ikebuchi M,Kimura H,Hill CK,Aoyama Tsubject
Has Abstractpub_date
1995-01-01 00:00:00pages
19-27issue
1eissn
0033-7587issn
1938-5404journal_volume
141pub_type
杂志文章abstract::Hypoxic BP-8 murine sarcoma cells were exposed to misonidazole and/or radiation and the kinetics and extent of cell death were evaluated with the [125I]iododeoxyuridine-prelabeling assay. Cell death after treatment with lethal doses of misonidazole was rapid and essentially complete within 2 or 3 days after drug expos...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::The dependence of relative biological effectiveness (RBE) on photon energy is a topic of extensive discussions. The increasing amount of in vitro data in the low-energy region indicates this to be a complex dependence that is influenced by the end point and cell line studied. In the present investigation, the RBE of 1...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0874.1
更新日期:2008-03-01 00:00:00
abstract::Statistical analysis of Monte Carlo simulations of positive ion track structure is presented for 1 MeV protons passing outside an absorbing region of interest. Energy deposition and production of ionization within the region occur only by delta-ray transport from the ion's path. Depositions in spherical absorber regio...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::We measured the production of reactive hydroxyl radical (OH.) by Fe2+ itself or complexed with nucleotide triphosphates or tripolyphosphate (TPP). Coumarin-3-carboxylic acid (3-CCA) reacts with the OH. produced by Fe2+, Fe3+ or Cu2+ plus ascorbate and with various iron complexes. We measured in real time the increased...
journal_title:Radiation research
pub_type: 杂志文章,评审
doi:
更新日期:1996-05-01 00:00:00
abstract::To evaluate whether DNA alterations in mature spermatozoa could stem from DNA damage induced in immature germ cells, testis cells and spermatozoa were analyzed by the comet assay and by the sperm chromatin structure assay 14, 45 and 100 days after in vivo X irradiation of the testes. These times were selected, accordi...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3053
更新日期:2003-10-01 00:00:00
abstract::Synchrotron microbeam radiation treatment (MRT) is a preclinical radiotherapy technique with considerable clinical promise, although some of the underlying radiobiology of MRT is still not well understood. In recently reported studies, it has been suggested that MRT elicits a different tumor immune profile compared to...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14115.1
更新日期:2016-01-01 00:00:00
abstract::It has been suggested that fibrosis which develops after irradiation is caused by increases in vascular permeability. Plasma proteins leak into irradiated tissue where fibrinogen may be converted into fibrin which is gradually replaced by fibrous tissue. Vascular and fibrotic changes in mouse lung were investigated af...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::To clarify the information in the Radiation Effects Research Foundation data regarding cancer risks of low radiation doses, we focus on survivors with doses less than 0.5 Sv. For reasons indicated, we also restrict attention mainly to survivors within 3, 000 m of the hypocenter of the bombs. Analysis is of solid cance...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)154[0178:rrcral]2.0.co;2
更新日期:2000-08-01 00:00:00
abstract::Fluorescence in situ hybridization procedures were used to examine the influence of chromosome locus on the frequency and type of chromosome aberrations induced by 60Co gamma rays in the human lymphoblastoid cell line TK6. Aberrations involving the X chromosome were compared to those involving the similarly sized auto...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::Reverse-biased silicon p-n junction arrays using Silicon-On-Insulator technology have been proposed as microdosimeters. The performance of such detectors in boron neutron capture therapy (BNCT) is discussed. This work provides the first reported measurements using boron-coated silicon diode arrays as microdosimeters i...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::The major limitation to reaching a curative radiation dose in radioresistant tumors such as malignant gliomas is the high sensitivity to radiation and subsequent damage of the surrounding normal tissues. Novel dose delivery methods such as minibeam radiation therapy (MBRT) may help to overcome this limitation. MBRT ut...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14018.1
更新日期:2015-09-01 00:00:00
abstract::We have used Escherichia coli B/r to test the proposal that hydroxyl radicals (.OH) are major contributors to lethal damage when bacteria in equilibrium with air or 100% nitrogen are exposed to ionizing radiation. In addition, we have tested the hypothesis that oxygen sensitizes bacterial cells to radiation by reactin...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-02-01 00:00:00
abstract::In a recent study (Int. J. Radiat. Oncol. Biol. Phys. 36, 635-639, 1996), 1H nuclear magnetic resonance (NMR) spectroscopy was used to demonstrate significant decreases in lactate levels after gamma irradiation of radiosensitive RIF-1 tumors in vitro. For comparison, we have examined the effects of gamma radiation on ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours following irradiation, oxid...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1968-11-01 00:00:00
abstract::Radiation nephropathy is one of the common late effects in cancer survivors who received radiotherapy as well as in victims of radiation accidents. The clinical manifestations of radiation nephropathy occur months after exposure. To date, there are no known early biomarkers to predict the future development of radiati...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14828.1
更新日期:2017-12-01 00:00:00
abstract::Ketogenic diets are low in carbohydrates and high in fat, which forces cells to rely more heavily upon mitochondrial oxidation of fatty acids for energy. Relative to normal cells, cancer cells are believed to exist under a condition of chronic mitochondrial oxidative stress that is compensated for by increases in gluc...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14668.1
更新日期:2017-06-01 00:00:00
abstract::Whether inner-shell ionizations of DNA atoms, called core ionizations, are critical events for cell inactivation by ionizing radiations such as 100 keV electrons and gamma rays has been investigated. The number of core ionizations in DNA atoms per gray of the two types of radiations is calculated from various Monte Ca...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0451.1
更新日期:2007-04-01 00:00:00
abstract::To ensure the high-fidelity transmission by reproductive cells of genetic information from generation to generation, cells have evolved surveillance systems to eliminate genomic lesions by inducing cell suicide and/or DNA repair. In this report, gamma-ray-induced cell death was investigated using the medaka fish, Oryz...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)157[0386:acsisf]2.0.co;2
更新日期:2002-04-01 00:00:00
abstract::We demonstrate by western analysis that the expression levels of TP53 (formerly known as p53), CDKN1A (formerly known as p21Waf1), CDC2 (formerly known as p34cdc2), CCNB1 (cyclin B1) and RAD51 are significantly modulated in confluent, density-inhibited human diploid cell populations exposed to doses where only a small...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Irradiation from internally deposited radionuclides induces malignant tumors. Ingested long-lived radionuclides accumulate in specific organs that are irradiated throughout life. To elucidate why the development of malignant tumors requires long-term internal exposure, of the order of decades, despite the fact that ir...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1492.1
更新日期:2009-04-01 00:00:00
abstract::The electroporation of restriction enzymes into mammalian cells results in DNA double-strand breaks that can lead to chromosome aberrations. Four chemicals known to interfere with cellular responses to DNA damage were investigated for their effects on chromosome aberrations induced by AluI and Sau3AI; in addition, the...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::The relative biological effectiveness was determined using sex-linked recessive lethals induced in Drosophila spermatozoa as the biological effect. The sex-linked recessive lethal test, a measure of mutations induced in germ cells and transmitted through successive generations, yields a linear dose-response curve in t...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract::The neon beam at 250 MeV/nucleon from the Bevalac at the Lawrence Berkeley Laboratory is bombarded in nuclear emulsion. The experimental interaction cross section of the neon beam in emulsion is 1078 +/- 35 mb, in agreement with the calculations from the modified Bradt and Peters formula. The secondary fragments were ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
abstract::Mortality data for B6CF1 mice exposed to 60Co gamma rays for the duration of life were used to make quantitative predictions of age-specific mortality observed in comparably exposed beagles. Simple Kaplan-Meier survival curves for the beagles and their 95% confidence intervals were computed for each dose-rate group ob...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::The intrinsic radiosensitivity of tumor cells is most frequently reported for asynchronous populations, although cell cycle variation in radiosensitivity is known to be significant. Linear-quadratic analyses of survival data for asynchronous human tumor cells show wide variations in the alpha coefficient with smaller ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::The present study was undertaken to assess the protective effect of dimethyl sulfoxide (DMSO) against the induction and rejoining of DNA double-strand breaks (DSBs) and inactivation of V79-4 Chinese hamster cells by both high- and low-linear energy transfer (LET) radiations. The cells were exposed under aerobic condit...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::Extension of previous investigations at this laboratory regarding life shortening and tumor induction in the mouse has provided more complete dose-response information in the low dose region of X rays and neutrons. A complete observation of survival and late pathology has been carried out on over 2000 BC3F1 female mic...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract::The capacity of plateau-phase Chinese hamster V79 and normal and transformed C3H-10T1/2 cells for repair of potentially lethal radiation damage (PLD) was evaluated for cells irradiated alone or given combined treatments of heat and radiation. The data show that all cell lines tested could repair PLD and that transform...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::We have previously observed time- and dose-dependent increases in matrix metalloproteinase 2 (Mmp2) protein levels in rat tubule epithelial cells (NRK52E) after irradiation. However, the mechanism(s) involved remains unclear. In the present study, irradiating NRK52E cells with 0-20 Gy gamma rays was associated with ti...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/3155
更新日期:2004-04-01 00:00:00
abstract::Technological developments in radiation therapy and other cancer therapies have led to a progressive increase in five-year survival rates over the last few decades. Although acute effects have been largely minimized by both technical advances and medical interventions, late effects remain a concern. Indeed, the need t...
journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/rr2530.1
更新日期:2012-04-01 00:00:00