Abstract:
:We demonstrate by western analysis that the expression levels of TP53 (formerly known as p53), CDKN1A (formerly known as p21Waf1), CDC2 (formerly known as p34cdc2), CCNB1 (cyclin B1) and RAD51 are significantly modulated in confluent, density-inhibited human diploid cell populations exposed to doses where only a small fraction of the nuclei are actually traversed by an alpha-particle track. The extent of modulation of TP53 and CDKN1A is significantly reduced in the presence of the gap junction inhibitor lindane and in irradiated low-density cell populations. In situ immunofluorescence studies show that at doses where about 2% of the nuclei would be traversed by an alpha particle, induction of CDKN1A occurs in more cells than predicted. Furthermore, the induced cells are present in isolated aggregates of neighboring cells. Therefore, our studies at the gene expression level indicate that similar signaling pathways are induced in bystander cells that are not traversed by an alpha particle as in traversed cells, and that biological effects in cell populations are not restricted to the response of individual cells to the DNA damage they receive.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Azzam EI,de Toledo SM,Gooding T,Little JBsubject
Has Abstractpub_date
1998-11-01 00:00:00pages
497-504issue
5eissn
0033-7587issn
1938-5404journal_volume
150pub_type
杂志文章abstract::Results from several laboratories, including ours, have suggested that measurements of radiation-induced DNA strand breaks and DNA-protein crosslinks (DPCs) may be used to estimate the hypoxic fraction or fractional hypoxic volume of tumors and normal tissues. This suggestion has been predicted on both published and u...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::Radiation is an independent risk factor for cardiovascular and cerebrovascular disease in cancer patients. Modern radiotherapy techniques reduce the volume of the heart and major coronary vessels exposed to high doses, but some exposure is often unavoidable. Radiation damage to the myocardium is caused primarily by in...
journal_title:Radiation research
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doi:10.1667/RR1862.1
更新日期:2010-12-01 00:00:00
abstract::In light-ion radiation therapy, both the dose and the local energy spectrum, which is often characterized with the linear energy transfer (LET), must be considered. In treatment optimization, it is advantageous to use a radiobiological model that analytically accounts for both dose and LET for the ion type of interest...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2045.1
更新日期:2010-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::The intrinsic radiosensitivity of tumor cells is most frequently reported for asynchronous populations, although cell cycle variation in radiosensitivity is known to be significant. Linear-quadratic analyses of survival data for asynchronous human tumor cells show wide variations in the alpha coefficient with smaller ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::Bcl-X(S) is a pro-apoptosis member of the Bcl2 family that has been shown to induce cell death and enhance chemosensitivity. We have investigated the effect of Bcl-X(S) overexpression on radiation sensitivity. Using a tetracycline-repressible system, we found that removal of tetracycline for 16 h induced Bcl-X(S) and ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3169
更新日期:2004-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2013-02-22 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1968-11-01 00:00:00
abstract::After exposure to 10 or 20 J/m2 UVC light, cells of the UMN-PIE-1181 line, an embryonic cell line derived from the Indian meal moth, Plodia interpunctella, exhibited a rapid and prolonged depression in the rate of incorporation of [3H]thymidine, whereas cells of the TN-368 line, an ovarian cell line derived from Trich...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR13351.1
更新日期:2013-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3522.1
更新日期:2006-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1541.1
更新日期:2009-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)156[0388:tkmfro]2.0.co;2
更新日期:2001-10-01 00:00:00
abstract::These studies examined the effects of X radiation and interleukin 3 (IL-3), which is an effective cytokine for the generation of megakaryocytopoiesis from X-irradiated hematopoietic stem/progenitor cells, on the terminal process of human megakaryocytopoiesis and thrombopoiesis. Mature megakaryocytes were induced by cu...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1519.1
更新日期:2009-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::The late effects of heavy-ion irradiation on the spinal cord of the rat were investigated histologically and morphometrically. After a single exposure of each animal's lower thoracic and lumbar spinal cord to a carbon-ion beam, the animals were observed clinically for up to 69 weeks and their spinal cords were examine...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章,评审
doi:
更新日期:1990-09-01 00:00:00
abstract::While the risk of lung cancer associated separately with smoking and radiation exposure has been widely reported, it is not clear how smoking and radiation together contribute to the risk of specific lung cancer histological types. With individual smoking histories and radiation dose estimates, we characterized the jo...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr2819.1
更新日期:2012-09-01 00:00:00
abstract::The severe combined immune deficiency (SCID) mouse shows two kinds of phenotypic abnormalities, a high radiosensitivity and an abnormal immunoglobulin gene recombination. A genetic study has revealed that a mutation exists in chromosome 16. However, several attempts to isolate the gene responsible for these phenotypes...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
abstract::The concept of curvature in dose-response relationships figures prominently in radiation biology, encompassing a wide range of interests including radiation protection, radiotherapy and fundamental models of radiation action. In this context, the ability to detect even small amounts of curvature becomes important. Sta...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14520.1
更新日期:2017-01-01 00:00:00
abstract::Ionizing radiation induces clustered DNA damage sites, whereby two or more individual DNA lesions are formed within one or two helical turns of DNA by a single radiation track. A subset of DNA clustered damage sites exist in which the lesions are located in tandem on the same DNA strand. Recent studies have establishe...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3284
更新日期:2005-01-01 00:00:00
abstract::To evaluate the function of the reticuloendothelial system and macrophage-monocyte system, blood samples from Thorotrast patients and age-matched healthy controls were used to determine the titer of plasma antibodies to lipid A and the numbers of several lymphocyte subclasses in peripheral blood and for an autoradiogr...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Human blood cultures were exposed to a 1.9 GHz continuous-wave (CW) radiofrequency (RF) field for 2 h using a series of six circularly polarized, cylindrical waveguides. Mean specific absorption rates (SARs) of 0.0, 0.1, 0.26, 0.92, 2.4 and 10 W/kg were achieved, and the temperature within the cultures during a 2-h ex...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)158[0523:ddamii]2.0.co;2
更新日期:2002-10-01 00:00:00