Abstract:
:Soil microorganisms in general and biocontrol agents in particular are very sensitive to UV light. The packaging of biocontrol microorganisms into cellular solids has been developed as a means of reducing loss caused by exposure to environmental UV radiation. The bacterial and fungal biocontrol agents Pantoea agglomerans and Trichoderma harzianum were immobilized in freeze-dried alginate beads containing fillers and subjected to 254 nm UV radiation (UVC). Immobilization of cells in freeze-dried alginate-glycerol beads resulted in greater survival after UV irradiation than for a free cell suspension. Adding chitin, bentonite or kaolin as fillers to the alginate-glycerol formulation significantly increased bacterial survival. Immobilization in alginate-glycerol-kaolin beads resulted in the highest levels of survival. The transmissive properties of the dried hydrocolloid cellular solid had a major influence on the amount of protection by the cell carrier. Dried alginate matrix (control) transmitted an average of 7.2% of the radiation. Filler incorporation into the matrix significantly reduced UV transmission: Alginate with kaolin, bentonite and chitin transmitted an average of 0.15, 0.38 and 3.4% of the radiation, respectively. In addition, the filler inclusion had a considerable effect on the bead's average wall thickness, resulting in a approximately 1.5- to threefold increase relative to beads based solely on alginate. These results suggest that the degree of protection of entrapped microorganisms against UVC radiation is determined by the UV-transmission properties of the dried matrix and the cellular solid's structure. It is concluded that for maximum protection against UV-radiation-induced cell loss, biocontrol microorganisms should be immobilized in alginate-glycerol beads containing kaolin.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Zohar-Perez C,Chernin L,Chet I,Nussinovitch Adoi
10.1667/rr3027subject
Has Abstractpub_date
2003-08-01 00:00:00pages
198-204issue
2eissn
0033-7587issn
1938-5404journal_volume
160pub_type
杂志文章abstract::Radiation exposure leads to a risk for long-term deterministic and stochastic late effects. Two individuals exposed to protracted photon radiation in the radiological accident at the Lilo Military site in Georgia in 1997 received follow-up treatment and resection of several chronic radiation ulcers in the Bundeswehr H...
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journal_title:Radiation research
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doi:
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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journal_title:Radiation research
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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