Abstract:
:Radiation-induced cell death is thought to be caused by nuclear DNA damage that cannot be repaired. However, in this study we found that a delayed increase of mitochondrial reactive oxygen species (ROS) is responsible for some of the radiation-induced cell death in normal human fibroblast cells. We have previously reported that there is a delayed increase of mitochondrial (·)O2(-), measured using MitoSOX™ Red reagent, due to gamma irradiation. This is dependent on Drp1 localization to mitochondria. Here, we show that knockdown of Drp1 expression reduces the level of DNA double-strand breaks (DSBs) remaining 3 days after 6 Gy irradiation. Furthermore, cells with knockdown of Drp1 expression are more resistant to gamma radiation. We then tested whether the delayed increase of ROS causes DNA damage. The antioxidant, 2-glucopyranoside ascorbic acid (AA-2G), was applied before or after irradiation to inhibit ROS production during irradiation or to inhibit delayed ROS production from mitochondria. Interestingly, 1 h after exposure, the AA-2G treatment reduced the level of DSBs remaining 3 days after 6 Gy irradiation. In addition, irradiated AA-2G-treated cells were more resistant to radiation than the untreated cells. These results indicate that delayed mitochondrial ROS production may cause some of the cell death after irradiation.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Kobashigawa S,Kashino G,Suzuki K,Yamashita S,Mori Hdoi
10.1667/RR13772.1subject
Has Abstractpub_date
2015-04-01 00:00:00pages
455-64issue
4eissn
0033-7587issn
1938-5404journal_volume
183pub_type
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更新日期:1993-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1988-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1988-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
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doi:
更新日期:1991-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章,评审
doi:
更新日期:1994-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1991-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
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