Abstract：
:We show here that the arrests of cells in G2 phase of the cell cycle induced by either staurosporine or ionizing radiation are closely related phenomena governed by a common kinase signaling pathway. The protein kinase inhibitor staurosporine induces a complete G2-phase arrest in exponentially growing TK6 human lymphoblastoid and V79 Chinese hamster fibroblast cells. Both cell types are equally sensitive to the kinase inhibitor and the arrest is dependent on its continued presence. Caffeine completely abrogates this arrest at concentrations comparable to those which abrogate radiation-induced G2-phase arrest. The kinetics of caffeine-induced release of both kinds of arrest are essentially identical. The activity of p34cdc2 kinase was also found to increase in a parallel fashion after caffeine-induced release of both kinds of arrest. As opposed to those transformed cell types which arrest only in G2 phase in response to staurosporine, immortalized C3H 10T1/2 fibroblasts and Muntjak skin fibroblasts display both G1- and G2-phase arrests. The results suggest that staurosporine and radiation interact with regulatory pathways in the cell cycle, and specifically with a caffeine-sensitive signal transduction pathway which recognizes DNA damage, regulates the G2/M-phase transition, and attenuates the biological consequences of radiation exposure.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Crompton NE,Hain J,Jaussi R,Burkart Wsubject
Has Abstractpub_date
1993-09-01 00:00:00pages
372-9issue
3eissn
0033-7587issn
1938-5404journal_volume
135pub_type
杂志文章abstract：:Hypoadrenocorticism, known as Addison's disease in humans, was diagnosed in six beagles after inhalation of at least 1.7 kBq/g lung of 238PuO2. Histological examination of adrenal gland specimens obtained at necropsy revealed marked adrenal cortical atrophy in all cases. Autoradiographs showed only slight alpha-partic...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Radiotherapy is one of the major treatment modalities for the management of various cancers, however, it is limited by the severe side effects and complications experienced by some patients. Nicaraven, a chemically synthesized hydroxyl radical-specific scavenger, has been shown to protect normal tissues from radiation...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14614.1
更新日期：2017-02-22 00:00:00
abstract：:Well-defined protocols and quality management standards are indispensable for biological dosimetry laboratories. Participation in periodic proficiency testing by interlaboratory comparisons is also required. This harmonization is essential if a cooperative network is used to respond to a mass casualty event. Here we p...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2425.1
更新日期：2011-05-01 00:00:00
abstract：:Experiments were performed with three different cell lines, mouse fibroblast LM cells, HeLa S3 cells, and Ehrlich Ascites Tumor (EAT) cells, to establish the possible importance of hyperthermic-induced alterations in cellular K+ content in the mechanism of cell killing by heat. At different time points after the hyper...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:We report here on a qualitative and quantitative comparison of four kinetic photon cell survival models. The commonly used linear-quadratic model extended by a dose protraction factor, the lethal potentially lethal model, the repair misrepair model and the recently reported Giant LOop Binary LEsion (GLOBLE) model are ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13862.1
更新日期：2015-11-01 00:00:00
abstract：:We have previously published the techniques and preliminary results of an SV40 viral probe assay for gamma-radiation-induced single- and double-strand DNA breaks and their intracellular repair in higher cells (Radiat. Res. 101, 356-372, 1985). Those experiments with SV40 infected CV-1 monkey kidney cells suggested tha...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:A significant fraction of DNA damage produced by ionizing radiation comes from free radicals generated during the radiolysis of water, that is, by indirect effects. The hydroxyl radical, the principal damaging species, produces single-strand breaks and a plethora of base and sugar lesions that can be cytotoxic or muta...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：
更新日期：1998-11-01 00:00:00
abstract：:Determinations of the LET distribution, phi(L), of charged particles within a spacecraft in low-Earth orbit have been made. One method used a cylindrical tissue-equivalent proportional counter (TEPC), with the assumption that for each measured event, lineal energy, y, is equal to LET and thus phi(L) = phi(y). The othe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)156[0310:coldmi]2.0.co;2
更新日期：2001-09-01 00:00:00
abstract：:The purpose of this study was to determine the antimutagenicity of WR-1065 added after irradiation of cells of cell lines differing in their ability to rejoin radiation-induced DNA double-strand breaks (DSBs). The postirradiation antimutagenicity of WR-1065 at the thymidine kinase locus was demonstrated for L5178Y (LY...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:Pentamidine is similar to rhodamine-123 in chemical structure and state of electron charge, and rhodamine-123 was previously shown to be a hyperthermic sensitizer under appropriate cell culture conditions. The present experiments were carried out to determine whether pentamidine would potentiate hyperthermic cell kill...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:The hyperthermic inhibition of cellular DNA synthesis, i.e., reduction in replicon initiation and delay in DNA chain elongation, was previously postulated to be involved in the induction of chromosomal aberrations believed to be largely responsible for killing S-phase cells. Utilizing asynchronous Chinese hamster ovar...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:Results from several laboratories, including ours, have suggested that measurements of radiation-induced DNA strand breaks and DNA-protein crosslinks (DPCs) may be used to estimate the hypoxic fraction or fractional hypoxic volume of tumors and normal tissues. This suggestion has been predicted on both published and u...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:1. A survival curve for the colony-forming ability of mouse bone marrow cells irradiated in vivo with Co60 gamma-rays has been obtained. The curve may be characterized by D0 of 95 rads and an extrapolation number of 1.5. 2. Comparison with the survival curve obtained for irradiated in vitro indicated a statistically s...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1962-06-01 00:00:00
abstract：:Exposure to ionizing radiation or a variety of chemical agents is known to increase the risk of developing malignancy and many tumors have been linked to inflammatory processes. In most studies, the potentially harmful effects of ionizing radiation or other agents are considered in isolation, mainly due to the large n...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2690.1
更新日期：2012-05-01 00:00:00
abstract：:The present study demonstrates the usefulness of immunochemical assays for quantitating modified bases in oxidized and X-irradiated DNA. Escherichia coli, phi X174 RF I, PM2, and M13 DNA containing thymine glycols introduced by OsO4 oxidation were used as antigens in a direct enzyme-linked immunosorbent assay (ELISA)....
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:Accurate assessment of risks of long-term space missions is critical for human space exploration. It is essential to have a detailed understanding of the biological effects on humans living and working in deep space. Ionizing radiation from galactic cosmic rays (GCR) is a major health risk factor for astronauts on ext...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15062.1
更新日期：2018-06-01 00:00:00
abstract：:New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours following irradiation, oxid...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-11-01 00:00:00
abstract：:Therapeutic gain factors (TGFs) have been determined for three spontaneous tumors of the C3H mouse treated by photons + normobaric oxygen (O2(1) ATA), photons + hyperbaric oxygen (O2 3 ATA), photons + misonidazole, or fast neutrons. The tumors were early generation isotransplants of spontaneous tumors: MCaIV, a mammar...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:To determine the linear energy transfer (LET) dependence of the biological effects of densely ionizing radiation in relation to changes in the ionization density along the track, we measured the yields and spectrum of clustered DNA damages induced by charged particles of different atomic number but similar kinetic ene...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2093.1
更新日期：2010-08-01 00:00:00
abstract：:The recent demonstration of nanoscale scintillators has led to interest in the combination of radiation and photodynamic therapy. In this model, scintillating nanoparticles conjugated to photosensitizers and molecular targeting agents would enhance the targeting and improve the efficacy of radiotherapy and extend the ...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/RR1470.1
更新日期：2009-02-01 00:00:00
abstract：:In boron neutron capture therapy, the absorbed dose from the (10)B(n,alpha)(7)Li reaction depends on the (10)B concentration and (10)B distribution in the irradiated volume. Thus compounds used in BNCT should have tumor-specific uptake and low accumulation in normal tissues. This study compares in a mouse model the (1...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1700.1
更新日期：2009-10-01 00:00:00
abstract：:Gemcitabine (dFdCyd) shows broad antitumor activity in solid tumors in chemotherapeutic regimens or when combined with ionizing radiation (radiosensitization). While it is known that mismatches in DNA are necessary for dFdCyd radiosensitization, the critical event resulting in radiosensitization has not been identifie...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14443.1
更新日期：2016-11-01 00:00:00
abstract：:The photodynamic properties of a second-generation photodynamic sensitizer, meta-tetra(hydroxyphenyl)chlorin (mTHPC) were studied by dye-sensitized photoinactivation (650 nm) of HT29 human adenocarcinoma cells in culture. The photocytotoxicity of mTHPC in vitro depended on the presence of molecular oxygen. A strong in...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:Exposure of humans and rodents to radiofrequency (RF) cell phone fields has been reported to alter a number of stress- related parameters. To study this potential relationship in more detail, tube-restrained immobilized Fischer 344 rats were exposed in the near field in a dose-dependent manner to pulse-modulated (11 p...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0584:eoiace]2.0.co;2
更新日期：2001-04-01 00:00:00
abstract：:A nested case-control study was conducted among workers at five U.S. nuclear facilities to evaluate leukemia mortality risk (excluding chronic lymphocytic) from ionizing radiation using worksite doses and adjusting for potential confounding. Conditional logistic regression was used to estimate the relative risk (RR) o...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr0724.1
更新日期：2007-02-01 00:00:00
abstract：:Previous studies identified radiation therapy as a key modifier of basal cell carcinoma (BCC) risk in survivors of hematopoietic cell transplantation (HCT). In the present analysis, risk of BCC was analyzed in relation to age at transplant, attained age, race, total-body irradiation (TBI), and radiation fractionation ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1469.1
更新日期：2009-02-01 00:00:00
abstract：:We have been investigating radiation-induced neoplastic transformants of C3H 10T1/2 mouse fibroblasts for evidence of heritable changes. C3H 10T1/2 cells were treated with 8 Gy X rays. After approximately 8 weeks of culture, type II/III foci were isolated from the monolayer using cloning rings. Cell lines developed fr...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:The placental transfer and distribution of 241Am in the feto-placental system were studied in pregnant rats. Rats were injected intravenously with 241Am citrate at 15 or 18 days of gestation. Groups injected at 15 days of gestation were sacrificed 2, 24, 48, or 120 hr after injection, and the group injected at 18 days...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-04-01 00:00:00
abstract：:While nontargeted and low-dose effects such as the bystander effect are now accepted, the mechanisms underlying the response have yet to be elucidated. It has been shown that the transfer of irradiated cell conditioned medium (ICCM) can kill cells that are not directly irradiated; however, to date the effect of ICCM c...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1141.1
更新日期：2008-02-01 00:00:00
abstract：:We have previously shown significant pathology in the heart and kidney of murine hematopoietic-acute radiation syndrome (H-ARS) survivors of 8.7-9.0 Gy total-body irradiation (TBI). The goal of this study was to determine temporal relationships in the development of vasculopathy and the progression of renal and cardio...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15130.1
更新日期：2019-05-01 00:00:00
