Abstract:
:Single crystals of guanine hydrobromide monohydrate, in which the guanine base is protonated at N7, were X-irradiated at 8 and 65 K. Using K-band ESR, ENDOR, and field-swept-ENDOR (FSE) techniques, the crystals were studied between 8 K and room temperature. There was evidence for five different radicals, RI-RV, immediately following irradiation at 8 or 65 K. RI was identified as the O6-protonated anion. It decayed near room temperature with no detectable successor. RII was identified as the N7-deprotonated cation, and decayed near 130 K. RIII is thought to be a ring-opened product formed by C8-N9 bond rupture; upon warming, it decayed at 150 K. RIV is the well-known C8 H-addition radical. These four radicals have been observed previously in the hydrochloride salt of guanine monohydrate. RV is novel, however, with magnetic characteristics consistent with those of the product formed by net OH addition to C5 of the unsaturated C4-C5 bond. It is characterized by four alpha-proton couplings indicating pi-electron spin as follows: 13% at C8; 11% at N7; and 12% at N10. RV decayed between 240 and 255 K with no detectable successor. Upon further warming, very weak resonance lines due to additional, unidentified radicals were observed. A comparison of these results with those obtained from other systems containing N7-protonated guanine bases demonstrates the effect of the environment on the primary radical formation.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Hole EO,Sagstuen E,Nelson WH,Close DMsubject
Has Abstractpub_date
1991-02-01 00:00:00pages
119-28issue
2eissn
0033-7587issn
1938-5404journal_volume
125pub_type
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journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
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pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1999-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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journal_title:Radiation research
pub_type: 杂志文章
doi:
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更新日期:2014-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1984-05-01 00:00:00
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