Abstract：
:The flow cytometry mutation assay is based on detecting mutations in the CD59 gene on human chromosome 11 in CHO A(L) cells with flow cytometry, but the kinetics of mutant expression and the histogram region for mutant selection have not been studied in detail. CHO A(L) cells were analyzed by flow cytometry for CD59 expression at various times after irradiation. The mutant fraction increased to a maximum at day 6 but decreased to near background levels by day 20. Cells were sorted from six different regions on the CD59 histograms after irradiation. The growth rate was similar for cells from all regions, and the surviving fraction was 50% of that for control cells. By 14 days the CD59 expression of cells from regions 2-5 was reduced to that of region 1. Cells were also analyzed for simultaneous expression of CD59, CD44 and CD90 (all on chromosome 11) to roughly characterize the size of the mutations. Triple mutants from the sorted populations were reduced from 41% on day 6 to 8% on day 24. We conclude that the mutant region should be increased to include cells with intermediate CD59 expression; also, the loss of CD59 mutant expression over time could be explained in part by the loss of triple mutants from the population.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Ross CD,Fox MHdoi
10.1667/RR1276.1subject
Has Abstractpub_date
2008-11-01 00:00:00pages
628-37issue
5eissn
0033-7587issn
1938-5404pii
RR1276journal_volume
170pub_type
杂志文章abstract：:The International Commission on Radiation Protection (ICRP) has lowered the dose limits for workers and for the general public exposed to ionizing radiation. Consequently, a reliable dosimetric method for monitoring possible radiation-induced damage is of great importance in radioprotection. The counting of dicentric ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3544.1
更新日期：2006-05-01 00:00:00
abstract：:New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours following irradiation, oxid...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-11-01 00:00:00
abstract：:Serum copper concentration was evaluated as an index of lung injury (monitored by lung prostacyclin production) with respect to the effects of time, dose, dose fractionation, and penicillamine dose modification in rats irradiated to the right hemithorax. Both lung PGI2 production and serum Cu concentration increased w...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:Male rabbits were exposed to a single irradiation dose of 1000-5000 rad when 12, 15, 16, and 17 months old and were killed when 18 months old (i.e., 1, 2, 3, and 6 months after irradiation). Even after this long interval after irradiation, abdominal aortic prostacyclin formation was significantly depressed, whereas th...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:The lineal energy distribution and several other dosimetric parameters were measured for the neutrons emitted from a replica of the Hiroshima bomb to determine their usefulness in biological experiments designed to estimate the effectiveness of actual Hiroshima neutrons. The "Little-Boy" replica (LBR) was constructed ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:The recent demonstration of nanoscale scintillators has led to interest in the combination of radiation and photodynamic therapy. In this model, scintillating nanoparticles conjugated to photosensitizers and molecular targeting agents would enhance the targeting and improve the efficacy of radiotherapy and extend the ...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/RR1470.1
更新日期：2009-02-01 00:00:00
abstract：:To evaluate the usefulness of the transgenic Muta mouse for investigating radiation-induced mutations in vivo, we have examined the effects of whole-body X irradiation and compared them to the effects of ultraviolet light. The spontaneous mutation frequencies in young adults were about 7 x 10(-5) in the spleen, liver ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:Ketogenic diets are low in carbohydrates and high in fat, which forces cells to rely more heavily upon mitochondrial oxidation of fatty acids for energy. Relative to normal cells, cancer cells are believed to exist under a condition of chronic mitochondrial oxidative stress that is compensated for by increases in gluc...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14668.1
更新日期：2017-06-01 00:00:00
abstract：:The health consequences of exposure to low-dose radiation combined with a solar particle event during space travel remain unresolved. The goal of this study was to determine whether protracted radiation exposure alters gene expression and oxidative burst capacity in the liver, an organ vital in many biological process...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1155.1
更新日期：2008-03-01 00:00:00
abstract：:Animal models of hematopoietic and gastrointestinal acute radiation syndromes (ARS) have been characterized to develop medical countermeasures. Acute radiation-induced decrease of intestinal absorptive function has been correlated to a decrease in the number of intestinal crypt cells resulting from apoptosis and enter...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14305.1
更新日期：2016-07-01 00:00:00
abstract：:Experiments were performed to test whether significant amounts of pyrimidine dimers are produced in cultured cells of tobacco (Nicotiana tabacum L. var. Xanthi) and of Haplopappus gracilis by ultraviolet light in the biological dose range and whether either or both dark and light repair systems exist in these cells. T...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-11-01 00:00:00
abstract：:Pepleomycin is a derivative of bleomycin, which is less toxic to the host but possesses greater antitumor activity. Experiments are described here in which Chinese hamster V-79 cells in culture and a murine squamous cell carcinoma in vivo have been used to obtain survival curves for pepleomycin alone or in combination...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:Mutation and killing induced by X radiation and 60CO gamma radiation were studied in six different histidine-requiring auxotrophs of Salmonella typhimurium. Strain TA100, which is sensitive to base-pair substitutions, and strains TA2637 and TA98, which are sensitive to frameshifts, carry the pKM101 plasmid and exhibit...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:The filter elution technique using nondenaturing conditions is widely used to assay DNA double-strand break (DSB) induction and repair. It has been reported that in the measurement of strand breaks higher rates of elution and of initial rejoining are obtained at pH 9.6 compared to pH 7.2. In the present experiments ne...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Incorporation of bromodeoxyuridine (BrdU) into DNA in both Chinese hamster ovary (CHO) cells and human melanoma (U1) cells reduced the rate of DNA migration in transverse alternating-field electrophoresis (TAFE) agarose gel optimized for separating molecules larger than 2 Mb. This "BrdU migration effect" was independe...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Radiation exposure causes DNA breaks leading to structural chromosome aberrations that can be carcinogenic. Lifetime cancer risks are elevated in irradiated children compared to similarly exposed adults. To determine the extent to which age influences the frequency and types of chromosome damage in response to ionizin...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13598.1
更新日期：2014-06-01 00:00:00
abstract：:The threat of exposure to ionizing radiation from a nuclear reactor accident or deliberate terrorist actions is a significant public health concern. The lung is particularly susceptible to radiation-induced injury from external sources or inhalation of radioactive particles from radioactive fallout. Radiation-induced ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14686.1
更新日期：2017-11-01 00:00:00
abstract：:The effect of extremely low-frequency electromagnetic field (ELF EMF) exposures to human health has been widely debated. Epidemiological studies have found a possible correlation between increased cancer incidence and environmental ELF EMF exposures. Results from in vitro studies performed to examine the possible unde...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)153[0663:eodctl]2.0.co;2
更新日期：2000-05-01 00:00:00
abstract：:Neurons are essential components of neural circuits and provide brain function organization. We previously reported that X irradiation induces apoptosis in immature neurons. To the best of our knowledge, there have been few reports investigating the effects of X irradiation on mature neurons. We analyzed the effects o...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3098.1
更新日期：2013-06-01 00:00:00
abstract：:In a scenario involving a nuclear detonation during war or a terrorist attack, acute radiation exposure combined with thermal and blast effects results in severe skin injury. Although the cutaneous injury in such a scenario may not be lethal, it may lead to inflammation, delayed wound healing and loss of the skin barr...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14884.1
更新日期：2018-04-01 00:00:00
abstract：:Deep-space travel presents risks of exposure to ionizing radiation composed of a spectrum of low-fluence protons (1H) and high-charge and energy (HZE) iron nuclei (e.g., 56Fe). When exposed to galactic cosmic rays, each cell in the body may be traversed by 1H every 3-4 days and HZE nuclei every 3-4 months. The effects...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14667.1
更新日期：2017-08-01 00:00:00
abstract：:The relative biological effectiveness was determined using sex-linked recessive lethals induced in Drosophila spermatozoa as the biological effect. The sex-linked recessive lethal test, a measure of mutations induced in germ cells and transmitted through successive generations, yields a linear dose-response curve in t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Radiation exposure from a number of terrestrial sources is associated with an increased risk for atherosclerosis. Recently, concern over whether exposure to cosmic radiation might pose a similar risk for astronauts has increased. To address this question, we examined the effect of 2 to 5 Gy iron ions ((56)Fe), a parti...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2482.1
更新日期：2011-06-01 00:00:00
abstract：:Human cancers have multiple alterations in cell signaling pathways that promote resistance to cytotoxic therapy such as X rays. Parthenolide is a sesquiterpene lactone that has been shown to inhibit several pro-survival cell signaling pathways, induce apoptosis, and enhance chemotherapy-induced cell killing. We invest...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1128.1
更新日期：2007-12-01 00:00:00
abstract：:Current therapeutic approaches for treatment of exposure to radiation involve the use of antioxidants, chelating agents, recombinant growth factors and transplantation of stem cells (e.g., hematopoietic stem cell transplantation). However, exposure to high-dose radiation is associated with severe damage to the vascula...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/RR14461.1
更新日期：2016-08-01 00:00:00
abstract：:The concept of curvature in dose-response relationships figures prominently in radiation biology, encompassing a wide range of interests including radiation protection, radiotherapy and fundamental models of radiation action. In this context, the ability to detect even small amounts of curvature becomes important. Sta...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14520.1
更新日期：2017-01-01 00:00:00
abstract：:In this study, we assessed the efficacy of unilamellar 110-nm liposomes encapsulating the chelating agent diethylenetriaminepentaacetic acid (DTPA) in plutonium-exposed rats. Rats were contaminated by intravenous administration of the soluble citrate form of plutonium. The comparative effects of liposomal and free DTP...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14968.1
更新日期：2018-05-01 00:00:00
abstract：:Vascular change by the combined treatments of radiation and hyperthermia was studied. The legs of rats were irradiated with 2000 rad of X rays in a single dose and heated at 43.0 degrees C for 1 hr at various times after irradiation, and the blood flow and vascular volume in the skin and muscle were determined with 12...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Irradiation of human melanoma (MeWo, Be11) and squamous cell carcinoma (4451, 4197) cells induces cell cycle blocks from which the cells recover to re-enter mitosis after 40-60 h. In the TP53 mutant cell lines, MeWo and 4451, irradiation induces a G(2)-phase block, where the fraction of cells in G(2) phase reaches a m...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)154[0640:aogmpb]2.0.co;2
更新日期：2000-12-01 00:00:00
abstract：:Radiation-induced neurotoxicity is a well-characterized phenomenon. However, the underlying mechanism of this toxicity is poorly understood. In the central nervous system (CNS), excitotoxic mechanisms are implicated in many neurodegenerative disease processes. Pivotal to the excitotoxic pathway is dysfunction of gluta...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2106.1
更新日期：2010-12-01 00:00:00
