Abstract:
:Current therapeutic approaches for treatment of exposure to radiation involve the use of antioxidants, chelating agents, recombinant growth factors and transplantation of stem cells (e.g., hematopoietic stem cell transplantation). However, exposure to high-dose radiation is associated with severe damage to the vasculature of vital organs, often leading to impaired healing, tissue necrosis, thrombosis and defective regeneration caused by aberrant fibrosis. It is very unlikely that infusion of protective chemicals will reverse severe damage to the vascular endothelial cells (ECs). The role of irradiated vasculature in mediating acute and chronic radiation syndromes has not been fully appreciated or well studied. New approaches are necessary to replace and reconstitute ECs in organs that are irreversibly damaged by radiation. We have set forth the novel concept that ECs provide paracrine signals, also known as angiocrine signals, which not only promote healing of irradiated tissue but also direct organ regeneration without provoking fibrosis. We have developed innovative technologies that enable manufacturing and banking of human GMP-grade ECs. These ECs can be transplanted intravenously to home to and engraft to injured tissues where they augment organ repair, while preventing maladaptive fibrosis. In the past, therapeutic transplantation of ECs was not possible due to a shortage of availability of suitable donor cell sources and preclinical models, a lack of understanding of the immune privilege of ECs, and inadequate methodologies for expansion and banking of engraftable ECs. Recent advances made by our group as well as other laboratories have breached the most significant of these obstacles with the development of technologies to manufacture clinical-scale quantities of GMP-grade and human ECs in culture, including genetically diverse reprogrammed human amniotic cells into vascular ECs (rAC-VECs) or human pluripotent stem cells into vascular ECs (iVECs). This approach provides a path to therapeutic EC transplantation that can be infused concomitantly or sequentially with hematopoietic stem cell transplantation more than 24 h after irradiation to support multi-organ regeneration, thereby improving immediate and long-term survival, while limiting long-term morbidity resulting from nonregenerative damage repair pathways.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Rafii S,Ginsberg M,Scandura J,Butler JM,Ding BSdoi
10.1667/RR14461.1subject
Has Abstractpub_date
2016-08-01 00:00:00pages
196-202issue
2eissn
0033-7587issn
1938-5404journal_volume
186pub_type
杂志文章,评审abstract::Radiation of the esophagus of C3H/HeNsd mice with 35 or 37 Gy of 6 MV X rays induces significantly increased RNA transcription for interleukin 1 (Il1), tumor necrosis factor alpha (Tnf), interferon gamma inducing factor (Ifngr), and interferon gamma (Ifng). These elevations are associated with DNA damage that is detec...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)155[0002:morice]2.0.co;2
更新日期:2001-01-01 00:00:00
abstract::An understanding of proton radiobiology is critical for optimization of both proton radiotherapy and assessment of carcinogenesis risk from space radiation. Although the physical aspects of proton beam radiobiology is well understood, the biological aspects, particularly the complex biological end points, have been un...
journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/RR2839.1
更新日期:2013-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0680.1
更新日期:2007-10-01 00:00:00
abstract::The effects of bromodeoxyuridine (BrdUrd) substitution for thymidine on gamma-ray-induced strand breakage were determined in single- and double-stranded oligonucleotides and double-stranded oligonucleotides containing a mismatched bubble region. BrdUrd does not sensitize complementary double-stranded DNA to gamma-ray-...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3267
更新日期:2004-12-01 00:00:00
abstract::The interaction function gamma(chi), which was introduced in the theory of dual radiation action as the probability that two energy transfers separated by distance chi combine with each other to produce a lesion, was estimated with sparsely ionizing radiation (60Co gamma rays and 40 kV X rays). Gamma(chi) was deduced ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::The binding site specificity of crosslinking mediated by the hydroxyl radical has been investigated in a simple model system: a tetrapeptide, Gly-Gly-Phe-Leu, and 14C-labeled phenylalanine. Crosslinking leads to the tetrapeptide-phenylalanine adduct which has been isolated by gel filtration. The amino acid analysis of...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-10-01 00:00:00
abstract::Most plutonium-induced bone tumors in beagles arise in trabecular bone sites surrounded by hematopoietic tissue. The microvasculature and the relative incorporation of 241Pu (a low energy beta emitter) on trabecular bone surfaces from red and fatty marrow sites were studied in the adult beagle. The vascular volume, as...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::Cytokines function in many roles that are highly relevant to radiation research. This review focuses on how cytokines are structurally organized, how they are induced by radiation, and how they orchestrate mesenchymal, epithelial and immune cell interactions in irradiated tissues. Pro-inflammatory cytokines are the ...
journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/RR3031.1
更新日期:2012-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)157[0386:acsisf]2.0.co;2
更新日期:2002-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1541.1
更新日期:2009-11-01 00:00:00
abstract::Impaired fluid and electrolyte transport in the intestine is a well-recognized characteristic of radiation-induced pathologies in the gastrointestinal tract. The aim of this study was to investigate the responsiveness of the epithelium of the colon of the rat to electrical and pharmacological (serotonin, carbachol) st...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Vascular change by the combined treatments of radiation and hyperthermia was studied. The legs of rats were irradiated with 2000 rad of X rays in a single dose and heated at 43.0 degrees C for 1 hr at various times after irradiation, and the blood flow and vascular volume in the skin and muscle were determined with 12...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::This study investigated whether the clonogenic ability of tumor cells to establish subcutaneous tumors and the tumor-associated macrophage (TAM) content correlate with the development and extent of the tumor bed effect (TBE). Ten tumors, five sarcomas and five carcinomas, syngeneic to C3Hf/Kam mice were used. Tumors w...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Chomentowski, M., Kellerer, A. M. and Pierce, D. A. Radiation Dose Dependences in the Atomic Bomb Survivor Cancer Mortality Data: A Model-Free Visualization. The standard approach to obtaining nominal risk coefficients for radiation-related cancer involves fitting linear or linear-quadratic dose-response functions. Th...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0289:rddita]2.0.co;2
更新日期:2000-03-01 00:00:00
abstract::The filter elution technique using nondenaturing conditions is widely used to assay DNA double-strand break (DSB) induction and repair. It has been reported that in the measurement of strand breaks higher rates of elution and of initial rejoining are obtained at pH 9.6 compared to pH 7.2. In the present experiments ne...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
abstract::Neither cell division nor growth of Saccharomyces cerevisiae were stimulated by the ultraviolet luminescence produced by adjacent exponential phase cultures of the yeast. The study included experiments in which the inocula (density = 5 X 10(7) cells cm-3) were irradiated and in which lag phase cultures (densities = 1 ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::This report compares the deterministic effects from an alpha-particle-emitting radionuclide, (239)PuO(2), and a beta-particle emitter, (144)Ce in fused aluminosilicate particles (FAP). The studies were conducted in beagle dogs of both genders exposed by inhalation to aerosols of the radionuclides. The initial lung bur...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Mixed radiation fields comprise the most common form of radiation exposure. Given their relevance in radiation protection, cancer radiotherapy and space research, accurate predictions of the corresponding radiation effects are essential. The local effect model (LEM) allows the prediction of cell survival after ion irr...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR15456.1
更新日期:2020-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)158[0110:aowilc]2.0.co;2
更新日期:2002-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3191
更新日期:2004-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
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journal_title:Radiation research
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更新日期:2010-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0535.1
更新日期:2006-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0752:ctitab]2.0.co;2
更新日期:2000-06-01 00:00:00
abstract::Clustered DNA damage is considered an important factor in determining the biological consequences of ionizing radiation. In this study, we successfully estimated the localization of abasic sites (APs) in DNA exposed to ionizing radiation using fluorescence resonance energy transfer (FRET) without any involvement of re...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR13780.1
更新日期:2015-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)155[0235:pcdtma]2.0.co;2
更新日期:2001-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1968-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::Many studies have shown the importance of altered cellular proto-oncogene expression in contributing to changes in cell survival, cell transformation, and cell cycle progression. In these experiments we examined the effects of total-body exposure of BCF1 mice to gamma rays (3 Gy) in modulating expression of cellular o...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00