Abstract:
:Clustered DNA damage is considered an important factor in determining the biological consequences of ionizing radiation. In this study, we successfully estimated the localization of abasic sites (APs) in DNA exposed to ionizing radiation using fluorescence resonance energy transfer (FRET) without any involvement of repair enzyme functions. A linearized plasmid (pUC19 digested by Sma I) was irradiated with: (60)Co γ rays; (4)He(2+) (2.0 MeV/u) particles; and the (12)C(5+) (0.37 MeV/u) particles in the solid state. A donor or acceptor fluorescent probe with a nucleophilic O-amino group was used to label APs. The results showed that the (12)C(5+) particle likely produced close APs within a track. The apparent distance calculated from the observed FRET efficiency (E) of around 0.10 was estimated to be approximately 17 base pairs. On the other hand, E values of (60)Co γ rays and the (4)He(2+) beam were less than those of the (12)C(5+) beam, increased with increasing AP density (the average number of APs per base pair), and were slightly greater than those of randomly distributed APs. We propose that the FRET method provides a degree of localization regardless of whether an AP cluster is single-stranded or bistranded DNA damage.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Akamatsu K,Shikazono N,Saito Tdoi
10.1667/RR13780.1subject
Has Abstractpub_date
2015-01-01 00:00:00pages
105-13issue
1eissn
0033-7587issn
1938-5404journal_volume
183pub_type
杂志文章abstract::Mice were irradiated with 1 Gy of fission neutrons. At intervals up to 15 days after irradiation undifferentiated spermatogonia were counted in whole mounts of seminiferous tubules in up to eight stages of the epithelial cycle. From Day 6 onward lower numbers of spermatogonia were found in the areas which were in stag...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
pub_type: 历史文章,杂志文章
doi:
更新日期:1996-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1759.1
更新日期:2010-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3085
更新日期:2003-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14653.1
更新日期:2018-01-01 00:00:00
abstract::Ionization generates a large number of secondary low-energy electrons (LEEs) with a most probable energy of approximately 10 eV, which can break DNA bonds by dissociative electron attachment (DEA) and lead to DNA damage. In this study, we investigated radiation damage to dry DNA induced by X rays (1.5 keV) alone on a ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14476.1
更新日期:2016-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2003)159[0775:eotsmf]2.0.co;2
更新日期:2003-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0479:pddihb]2.0.co;2
更新日期:2000-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Ionizing radiation is known to effect development during early life stages. Lake whitefish (Coregonus clupeaformis) represent a unique model organism for examining such effects. The purpose of this study was to examine how ionizing radiation affects development in lake whitefish embryos and to investigate the presence...
journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2017-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
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pub_type: 杂志文章,评审
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