Abstract：
:The effect of ionizing radiation (137Cs) on processing and transport of ribosomal RNA (rRNA) was studied by pulse-labeling HeLa S3 cells with [3H]uridine immediately prior to irradiation. This approach permits kinetic analysis of processing of 45 S rRNA (radiolabeled predominantly prior to irradiation) into its 28 S and 18 S rRNA daughter species following irradiation. By this technique, we have recently demonstrated an increase in the normal 28 S:18 S rRNA stoichiometric ratio of 1:1 to as high as 1.6:1 during the interval 5 to 20 h following irradiation of HeLa cells at greater than or equal to 7.5 Gy. Alterations in 28 S:18 S ratio were evaluated in greater detail at early times following irradiation, up to 2 h. The 28 S:18 S ratio was found to be maximal at 1 h after radiation, at about 2:1, following 5 or 10 Gy. Using a method for rapid separation of nucleus from cytoplasm, transport of rRNA from nucleus to cytoplasm was also evaluated during this period. Despite an increase in the rate of 45 S rRNA processing, as well as an increased 28 S:18 S ratio, no alterations in transport from nucleus to cytoplasm were detected. This lack of transport alteration suggests that accumulation of excess 28 S rRNA is restricted to the nucleus, where it may represent an early step in the process of radiation-induced cell killing.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Fuchs P,Krolak JM,McClain D,Minton KWsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
67-70issue
1eissn
0033-7587issn
1938-5404journal_volume
121pub_type
杂志文章abstract：:The retention of 241Am in mice 48 h after administration by gavage is reported here. The 241Am was given to mice in the form of either 241Am nitrate or 241Am citrate. The 241Am was also injected into rats in the same form. The homogenized livers of those rats were subsequently administered by gavage to another group o...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:After total-body exposure to various doses of ionizing radiation, the ability of lymphocytes to interact specifically with high endothelial venules of rat cervical and mesenteric lymph nodes was analyzed in frozen sections. Following a radiation dose of 1.5 Gy, high endothelial venules remained intact and the binding ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:The concept of curvature in dose-response relationships figures prominently in radiation biology, encompassing a wide range of interests including radiation protection, radiotherapy and fundamental models of radiation action. In this context, the ability to detect even small amounts of curvature becomes important. Sta...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14520.1
更新日期：2017-01-01 00:00:00
abstract：:Molecular imaging is an evolving science that is concerned with the development of novel imaging probes and biomarkers that can be used to non-invasively image molecular and cellular processes. This special issue approaches molecular imaging in the context of radiation research, focusing on biomarkers and imaging meth...
journal_title：Radiation research
pub_type：
doi：10.1667/rr2959.1
更新日期：2012-04-01 00:00:00
abstract：:Little computational or experimental information is available on site-specific hydroxyl attack probabilities to DNA. In this study, an atomistic stochastic model of OH radical reactions with DNA was developed to compute relative OH attack probabilities at individual deoxyribose hydrogen atoms. A model of the self-comp...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0038:ssoatt]2.0.co;2
更新日期：2002-01-01 00:00:00
abstract：:Our data (Reddy et al., Radiat. Res. 141, 252-258, 1995) on the kinetics of the repair of potentially lethal damage in log-phase Chinese hamster V79 cells are used to test some predictions which arise from the different assumptions of the repair-misrepair (RMR) (C. A. Tobias, Radiat. Res. 104, S77-S95, 1985), lethal-p...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Currently available treatments for radiation-induced nausea and vomiting either are ineffective or reduce performance. The new antiemetic and gastrokinetic agent zacopride was tested in rhesus monkeys to assess its behavioral toxicity and its ability to inhibit radiation-induced emesis. Zacopride (intragastric, 0.3 mg...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:Long-term retention of 133Ba in the trachea from intratracheally administered BaSO4 particles was determined by both serial sacrifice and external scanning methods up to 6 months after injection. The amount of 133Ba retained 1 week after injection in the caudal region of the trachea, where the tip of the cannula had b...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:Cells plated immediately after irradiation on nutrient agar (immediate plating) exhibit a lower survival than cells which are kept under nongrowth conditions before plating (delayed plating). The difference between the survival curves obtained after immediate plating and delayed plating is considered to exhibit the ce...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:Previous work from our laboratory suggested that neuronal chromatin structure may be altered immediately after exposure to ionizing radiation. In the present study, whole brains of 4-month-old male Fisher 344 rats were irradiated with a dose of 25 Gy. The kinetics of restoring the chromatin structure to its unirradiat...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:The high-energy neutron component of the space radiation environment in thick structures such as the International Space Station contributes to the total radiation dose received by an astronaut. Detector design constraints such as size and mass have limited the energy range of neutron spectrum measurements in orbit to...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2003)159[0154:henswt]2.0.co;2
更新日期：2003-02-01 00:00:00
abstract：:We studied the spatial and temporal distributions of foci of the phosphorylated form of the histone protein H2AX (gamma-H2AX), which is known to be activated by double-strand breaks after irradiation of human fibroblast cells with high-energy silicon (54 keV/microm) and iron (176 keV/microm) ions. Here we present data...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3431.1
更新日期：2005-10-01 00:00:00
abstract：:Transforming growth factor-β-activated kinase 1 (TAK1) appears to play a role in inhibiting apoptotic death in response to multiple stresses. To assess the role of TAK1 in X-ray induced apoptosis and cell death, we irradiated parental and siRNA-TAK1-knockdown HeLa cells. Changes in gene expression levels with and with...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2792.1
更新日期：2012-06-01 00:00:00
abstract：:Dose-spread kernels can be used to calculate the dose distribution in a photon beam by convolving the kernel with the primary fluence distribution. The theoretical relationships between various types and components of dose-spread kernels relative to photon attenuation coefficients are explored. These relations can be ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:This study was designed to determine changes in cell numbers, proliferation (using Ki-67) and EGFR expression in mouse bladder urothelium during the early and late radiation response. Groups of mice were irradiated with a single dose of 20 Gy and assayed 0-360 days later. Urothelial cells were counted. After immunohis...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1759.1
更新日期：2010-04-01 00:00:00
abstract：:To study the acquired radioresistance of tumor cells, a model system of two cell lines, Djungarian hamster fibroblasts (DH-TK-) and their radioresistant progeny, was established. The progeny of irradiated cells were isolated by treating the parental cell monolayer with a single dose of 20 Gy (PIC-20). The genetic and ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3443.1
更新日期：2005-12-01 00:00:00
abstract：:We investigated the regeneration of rat thymus after sublethal X irradiation (6 Gy). The number of thymocytes was much lower on day 3 after irradiation, and many apoptotic cells were observed. However, by day 5, there had been a rapid proliferation of thymocytes. Since cytokines are considered to be important regulato...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0281:iaaicr]2.0.co;2
更新日期：2002-03-01 00:00:00
abstract：:The German Thorotrast study comprises 2,326 patients and 1,890 controls. Forty-eight Thorotrast patients and 239 controls are still alive and are invited for a follow-up examination every 2 years. In the deceased patients, the following neoplastic diseases with excess rates were registered (Thorotrast/controls): liver...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:A standardized dichlorofluorescin (DCF) fluorometric assay capable of measuring radiation-induced oxidative stress was used to determine the effectiveness of protons and high-mass, high-atomic number (Z) and high-energy (HZE) particles to produce oxidative stress in vitro. Protons were found to be about equally as eff...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2005)163[0364:doosib]2.0.co;2
更新日期：2005-04-01 00:00:00
abstract：:Involvement of gap junctional intercellular communication (GJIC) in bystander responses of confluent human fibroblasts irradiated with a carbon-ion beam was investigated. It was found that the lower the radiation dose, the higher the yield of radiation-induced micronuclei per nuclear traversal, suggesting the existenc...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3044
更新日期：2003-09-01 00:00:00
abstract：:Gemcitabine (dFdCyd) shows broad antitumor activity in solid tumors in chemotherapeutic regimens or when combined with ionizing radiation (radiosensitization). While it is known that mismatches in DNA are necessary for dFdCyd radiosensitization, the critical event resulting in radiosensitization has not been identifie...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14443.1
更新日期：2016-11-01 00:00:00
abstract：:We have investigated tumor response to low-dose-rate irradiation from an implanted 125I source alone or in conjunction with intratumoral drug administration. The drug (cis-DDP or 5-FU) was incorporated homogeneously into the co-polymer CPP-SA, 20:80, and the polymer/drug rods were implanted in the RIF-1 fibrosarcomas ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3204
更新日期：2004-07-01 00:00:00
abstract：:We have previously observed time- and dose-dependent increases in matrix metalloproteinase 2 (Mmp2) protein levels in rat tubule epithelial cells (NRK52E) after irradiation. However, the mechanism(s) involved remains unclear. In the present study, irradiating NRK52E cells with 0-20 Gy gamma rays was associated with ti...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/3155
更新日期：2004-04-01 00:00:00
abstract：:The increasing use of mobile phones by children and teenagers has raised concerns about their safety. Addressing such concerns is difficult, because no data are available on possible effects from long-term exposure to radiofrequency (RF) fields during the development of the nervous system. Possible morphological and f...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1002.1
更新日期：2007-10-01 00:00:00
abstract：:Mice were irradiated with 1 Gy of fission neutrons. At intervals up to 15 days after irradiation undifferentiated spermatogonia were counted in whole mounts of seminiferous tubules in up to eight stages of the epithelial cycle. From Day 6 onward lower numbers of spermatogonia were found in the areas which were in stag...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:The effect of X irradiation on the respiration of rat thymocytes was studied. An increase in the rate of O2 uptake was observed 1 h after cells were irradiated with doses of 6-10 Gy. The radiation-induced increase in respiration could be blocked by oligomycin, an inhibitor of mitochondrial ATP synthase, suggesting con...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:The effect of both hyperthermia and verapamil on cytotoxicity and transport of melphalan was studied in a pleiotropic drug-resistant Chinese hamster ovary cell line (CHRC5) and in the drug-sensitive parent line (AuxB1). The CHRC5 cell line was selected for resistance to colchicine but is also cross-resistant to other ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:The study design and obtained results represent an intercomparison of various laboratories performing dose assessment using the dicentric chromosome analysis (DCA) as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibrat...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3235.1
更新日期：2013-08-01 00:00:00
abstract：:Wistar rats (36) were exposed daily throughout pregnancy to a power density level of 35 mW/cm2 of 6000-MHz microwave radiation (11), sham irradiated (10), or used as control animals (15). Litters were culled to a maximum of eight F1a offspring/litter (total = 124) on Postnatal Day 1 and subjected to a series of reflex...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-02-01 00:00:00
abstract：:Abstract Double-strand breaks (DSBs) can be generated in the DNA of mammalian cells when heat-labile sites induced by ionizing radiation within a clustered DNA damage site are thermally transformed to single-strand breaks (SSBs) and combine with other SSBs or heat-labile sites in the opposite DNA strand. When this the...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1745.1
更新日期：2009-08-01 00:00:00
