Abstract：
:Previously, we described the radiation-induced (RI) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) effect as the increased MTT metabolization at the intermediate dose region after the irradiation of an MCF-7/6 cell monolayer with an X-ray dose gradient. We wondered if the cell monolayer at the intermediate dose region was characterized by an increased metabolic activity. In this study, we unraveled the mechanisms behind the RI MTT effect. Comparison of the MTT, sulforhodamine B (SRB), 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium (WST-8), and nitroblue tetrazolium (NBT) assays indicated that the RI MTT effect is not due to an increased cell density, but to an exclusively intracellular MTT conversion. Our results for the MTT and NBT assays after digitonin pretreatment of the irradiated cell monolayer indicated a role of the plasma membrane permeability in the RI MTT effect. Assessment of the radiation impact on the oxidative phosphorylation system by Western blot analysis, spectrophotometric measurement and Blue Native gel electrophoresis showed a dose-dependent downregulation of the oxidative phosphorylation system complexes, whereby the radiosensitivity of each complex was proportional to the number of mitochondrial DNA-encoded subunits. Further, only treatment of the irradiated cell monolayer with a cocktail and not with the individual inhibitors of complexes I, II and IV during the MTT assay prevented the RI MTT effect. In general, our results demonstrate that the RI MTT effect is not due to an increased metabolic activity, but rather to an enhanced cellular MTT entry and mitochondrial MTT conversion.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Blockhuys S,Vanhoecke B,Smet J,De Paepe B,Van Coster R,Bracke M,De Wagter Cdoi
10.1667/RR3070.1subject
Has Abstractpub_date
2013-04-01 00:00:00pages
433-43issue
4eissn
0033-7587issn
1938-5404journal_volume
179pub_type
杂志文章abstract：:To determine the linear energy transfer (LET) dependence of the biological effects of densely ionizing radiation in relation to changes in the ionization density along the track, we measured the yields and spectrum of clustered DNA damages induced by charged particles of different atomic number but similar kinetic ene...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2093.1
更新日期：2010-08-01 00:00:00
abstract：:Infusions of boronophenylalanine-fructose complex (BPA-F), at doses up to 900 mg/kg of BPA and 860 mg/kg of fructose, have been used to deliver boron to cancer tissue for boron neutron capture therapy (BNCT). In patients with phenylketonuria (PKU), phenylalanine accumulates, which is harmful in the long run. PKU has b...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3067
更新日期：2003-11-01 00:00:00
abstract：:In an effort to identify proteomic changes that may be useful for radiation biodosimetry, human cells of hematological origin were treated with ionizing radiation or mock-irradiated and then harvested at different times after treatment. Protein lysates were generated from these cells and evaluated by Western blotting ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1638.1
更新日期：2009-05-01 00:00:00
abstract：:The immunocytochemical response to DNA damage induced by low-energy bismuth and carbon ions was investigated in normal human fibroblasts. Inside the nuclei, the traversing charged particles lead to the accumulation of proteins related to DNA lesions and repair along the ion trajectories. Irradiation under a standard g...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2003)159[0676:biohct]2.0.co;2
更新日期：2003-05-01 00:00:00
abstract：:These assessment of the consequences of irradiation on chromatin is complicated by endogenous nucleases. Isolation and prolonged storage of rat liver nuclei in buffers containing divalent metal ions activates these enzymes and promotes the degradation of chromatin. Irradiation of rat liver nuclei to dose levels of 20,...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:The potential carcinogenic effects of internally deposited alpha-particle-emitting nuclides, notably plutonium, in the liver in humans are unknown but are of concern in relation to exposures from the nuclear industry. However, patients injected with the radiographic contrast medium Thorotrast are chronically exposed t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:To elucidate the nature and kinetics of DNA strand breaks caused by low-energy Auger electron emitters, we compared the yields of DNA breaks in supercoiled pUC19 DNA in the presence of the (.)OH scavenger dimethyl sulfoxide (DMSO) after the decay of (125)I (1) in proximity to DNA after minor-groove binding ((125)I-iod...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:The risk of a radiological or nuclear public health emergency is a major growing concern of the U.S. government. To address a potential incident and ensure that the government is prepared to respond to any subsequent civilian or military casualties, the U.S. Department of Health and Human Services and the Department o...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15137.1
更新日期：2018-12-01 00:00:00
abstract：:Bcl-X(S) is a pro-apoptosis member of the Bcl2 family that has been shown to induce cell death and enhance chemosensitivity. We have investigated the effect of Bcl-X(S) overexpression on radiation sensitivity. Using a tetracycline-repressible system, we found that removal of tetracycline for 16 h induced Bcl-X(S) and ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3169
更新日期：2004-05-01 00:00:00
abstract：:The present paper describes experiments conducted over the last several years which have focused primarily on the development of mammary tumors in BALB/c mice after neutron and gamma irradiation. Time-dose relationships for induction of mammary and lung tumors following irradiation with fission-spectrum neutrons and 1...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:Chinese hamster ovary cells were irradiated with 250 kVp X rays and analyzed for the presence of DNA double-strand breaks using either polycarbonate filter elution or pulsed-field agarose gel electrophoresis at neutral pH. Reduction in DNA length detected by filter elution was produced as a nonlinear function of incre...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Deep underground laboratories (DULs) were originally created to host particle, astroparticle or nuclear physics experiments requiring a low-background environment with vastly reduced levels of cosmic-ray particle interference. More recently, the range of science projects requiring an underground experiment site has gr...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15083.1
更新日期：2018-09-01 00:00:00
abstract：:Radioprotection in vitro by sulfhydryl (SH)-containing compounds is usually greater in aerated than in hypoxic cells. This observation has been cited recently as one of the reasons for the relatively greater effectiveness of radioprotectors such as WR-2721 in normal tissues compared to tumor cells. It is demonstrated ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:Puskin, J. S. What Can Epidemiology Tell Us about Risks at Low Doses? Radiat. Res. 169, 122-124 (2008). Limitations on statistical power preclude direct detection and quantification of radiogenic cancer risks at very low (environmental) levels of low-LET radiation through epidemiological studies. Given this limitation...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1187.1
更新日期：2008-01-01 00:00:00
abstract：:The late effects of heavy-ion irradiation on the spinal cord of the rat were investigated histologically and morphometrically. After a single exposure of each animal's lower thoracic and lumbar spinal cord to a carbon-ion beam, the animals were observed clinically for up to 69 weeks and their spinal cords were examine...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Single crystals of the co-crystalline complex of 1-methyluracil and 9-ethyladenine were X-irradiated and studied using EPR, ENDOR and FSE spectroscopic techniques at 10 K. All together seven radicals were identified, and experimental evidence for at least one more species, as well as for a very low population of radic...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:Determinations of the LET distribution, phi(L), of charged particles within a spacecraft in low-Earth orbit have been made. One method used a cylindrical tissue-equivalent proportional counter (TEPC), with the assumption that for each measured event, lineal energy, y, is equal to LET and thus phi(L) = phi(y). The othe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)156[0310:coldmi]2.0.co;2
更新日期：2001-09-01 00:00:00
abstract：:The mutagenic activity of green (532 nm) and infrared (1064 nm) ultra-short laser light pulses was tested in cultured Syrian hamster fibroblasts by a hypoxanthine phosphoribosyl-transferase (HPRT) mutagenesis assay. In 18 irradiation trials, cells were exposed to eight consecutive 100-ps pulses of either 532 nm or 106...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:The purpose of this work was to determine how fractionated radiation used in the treatment of tumors affects the ability of cancer as well as normal cells to repair induced DNA double-strand breaks (DSBs) and how cells that have lost this ability die. Lymphocytic leukemia cells (MOLT4) were used as an experimental mod...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2478.1
更新日期：2011-06-01 00:00:00
abstract：:We report results for chromosomal aberrations in human peripheral blood lymphocytes after they were exposed to high-energy iron ions with or without shielding at the HIMAC, AGS and NSRL accelerators. Isolated lymphocytes were exposed to iron ions with energies between 200 and 5000 MeV/nucleon in the 0.1-1-Gy dose rang...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3362.1
更新日期：2005-10-01 00:00:00
abstract：:Cognitive impairments after brain irradiation seriously affect quality of life for patients, and there is currently no effective treatment. In this study using an irradiated rat model, the role of electroacupuncture was investigated for treatment of radiation-induced brain injury. Animals received 10 Gy exposure to th...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14561.1
更新日期：2017-06-01 00:00:00
abstract：:Carcinogenesis is a multistep process, involving the irreversible conversion of a stem cell to a terminal-differentiation-resistant cell ("initiation"), followed by the clonal expansion of this cell ("promotion") and by the acquisition of other genetic alterations leading to malignancy ("progression"). The initiation ...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：
更新日期：1990-09-01 00:00:00
abstract：:The linear no-threshold extrapolation from a dose-response relationship for ionizing radiation derived at higher doses to doses for which regulatory standards are proposed is being challenged by some scientists and defended by others. It appears that the risks associated with exposures to doses of interest are below t...
journal_title：Radiation research
pub_type：
doi：
更新日期：1998-12-01 00:00:00
abstract：:Individual differences in response to radiation are well known, but the molecular basis for these differences is not well understood, and molecular indicators that are useful in assessing individual variation are lacking. Cells from patients developing unexpected radiation responses have occasionally been analyzed for...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)156[0668:gerilc]2.0.co;2
更新日期：2001-11-01 00:00:00
abstract：:The influence of Trp53 on the radiation-induced elevation of T-cell receptor (TCR) variant fractions was examined in splenic T lymphocytes of Trp53-proficient and -deficient mice. Wild-type Trp53+/+, heterozygous Trp53+/- and null Trp53-/- mice were exposed to 3 Gy of X rays at 8 weeks of age. The fraction of TCR-defe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3583.1
更新日期：2006-07-01 00:00:00
abstract：:The appearance of prostaglandins and thromboxane in mouse serum after X irradiation was observed by simultaneous quantitative analysis using gas chromatography/mass spectrometry/selected ion monitoring with stable isotope dilution methods. Mice of two strains (C57BL/CN Jcl and BALB/c) showed similar responses to X irr...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:We studied the spatial and temporal distributions of foci of the phosphorylated form of the histone protein H2AX (gamma-H2AX), which is known to be activated by double-strand breaks after irradiation of human fibroblast cells with high-energy silicon (54 keV/microm) and iron (176 keV/microm) ions. Here we present data...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3431.1
更新日期：2005-10-01 00:00:00
abstract：:As part of a long-term study of the effects of irradiation during development, prenatal and early neonatal mortality were evaluated for beagles exposed in utero at 8 days postcoitus (dpc), 28 dpc, 55 dpc, or 2 days postpartum. Mean doses used were 0,0.16, or 0.83 Gy. A decrease in whelping rates was observed for femal...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:A novel technique has been employed to investigate the simultaneous damage to DNA components induced by soft X rays (1.5 keV) and low-energy electrons (0-30 eV) in thin films of thymidine deposited on glass and tantalum substrates and irradiated under atmospheric pressure and temperature. The films were surrounded by ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13584.1
更新日期：2014-06-01 00:00:00
abstract：:Identification of medical countermeasures (MCM) to mitigate radiation damage and/or protect first responders is a compelling unmet medical need. The prostaglandin E2 (PGE2) analog, 16,16 dimethyl-PGE2 (dmPGE2), has shown efficacy as a radioprotectant and radiomitigator that can enhance hematopoiesis and ameliorate int...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RADE-20-00181.1
更新日期：2020-12-10 00:00:00
