Abstract:
:These assessment of the consequences of irradiation on chromatin is complicated by endogenous nucleases. Isolation and prolonged storage of rat liver nuclei in buffers containing divalent metal ions activates these enzymes and promotes the degradation of chromatin. Irradiation of rat liver nuclei to dose levels of 20,000 rad under conditions in which endogenous nucleases are inhibited and analysis of the irradiated chromatin by sucrose density gradient centrifugation gave no evidence for monosomes or oligosomes. When chromatin from irradiated nuclei was digested with micrococcal nuclease, the levels of monosomes and oligosomes were identical to those of micrococcal nuclease, the levels of monosomes and oligosomes are identical to suggest that irradiation results in neither a direct fragmentation of linkers nor the sensitization of linkers for subsequent cleavage by micrococcal nuclease. Histones isolated from monosomes of irradiated and unirradiated nuclei were intact, showing no fragmentation or loss of residues, as judged by sodium dodecyl sulfate-polyacrylamide electrophoresis.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Gelderblom D,Smit BJ,Böhm Lsubject
Has Abstractpub_date
1984-08-01 00:00:00pages
363-71issue
2eissn
0033-7587issn
1938-5404journal_volume
99pub_type
杂志文章abstract::The space environment consists of a varying field of radiation particles including high-energy ions, with spacecraft shielding material providing the major protection to astronauts from harmful exposure. Unlike low-LEpsilonTau gamma or X rays, the presence of shielding does not always reduce the radiation risks for en...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0535.1
更新日期:2006-10-01 00:00:00
abstract::This study was undertaken to determine if structural alterations of the bulk chromatin and the amount of protein associated with the nuclear matrix in cerebellar neurons depend on radiation dose and a cell's state of oxygenation. After irradiation with 2.5 to 25.0 Gy under both aerobic and anoxic conditions, the sensi...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract::A recent publication by Leith et al. (Cancer Res. 51, 4111-4113, 1991) showed that administration of epidermal growth factor (EGF) (0.25 mg/kg; q.i.d. x 7) to mice bearing xenografted A431 human epidermoid cancers produced increased tumor growth and a reduction of the percentage of hypoxic cells within neoplasms. In c...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::Three somatic mutation assays were evaluated in men exposed to low-dose, whole-body, ionizing radiation. Blood samples were obtained between 1992 and 1999 from 625 Russian Chernobyl cleanup workers and 182 Russian controls. The assays were chromosome translocations in lymphocytes detected by FISH, hypoxanthine phospho...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)158[0424:tsgbac]2.0.co;2
更新日期:2002-10-01 00:00:00
abstract::The effect of ionizing radiation (137Cs) on processing and transport of ribosomal RNA (rRNA) was studied by pulse-labeling HeLa S3 cells with [3H]uridine immediately prior to irradiation. This approach permits kinetic analysis of processing of 45 S rRNA (radiolabeled predominantly prior to irradiation) into its 28 S a...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::We investigated delayed reproductive death and chromosome bridge formation in mitotic cells in colonies of normal human embryonic cells which survived exposure to 6 Gy of X rays. Eighteen primary clones each derived from control and irradiated cultures were isolated and secondary colonies were grown up. Six of the pri...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::We tested the possibility that hyperthermia kills HA-1 cells in a manner analogous to growth factor deprivation. HA-1 cells were inactivated by serum starvation when incubated in Eagle's MEM at a density of 40 cells/cm2 or less. Cells became resistant to the absence of serum when the cell density was greater than 400 ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract::Human keratinocytes show a bystander effect when exposed to low doses of low-LET radiation. In this paper, data are presented showing a method of correcting the overall survival curve to enable analysis of the relative contributions of the bystander effect and the effect attributable to direct interaction of the radia...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0508:rcobat]2.0.co;2
更新日期:2000-05-01 00:00:00
abstract::Radon is recognized as a public health concern for indoor exposure. Precise quantification derived from occupational exposure in miners is still needed for estimating the risk and the factors that modify the dependence on cumulated exposure. The present paper reports on relationship between radon exposure and lung can...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0848.1
更新日期:2008-02-01 00:00:00
abstract::CD34 antigen is a novel marker for human hematopoietic stem/progenitor cells. In the present study, two cell fractions, CD34low and CD34high, were prepared from steady-state human peripheral blood on the basis of CD34 antigen expression. The colony-forming unit megakaryocytes (CFU-Meg) contained in each cell fraction ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3396
更新日期:2005-07-01 00:00:00
abstract::Single crystals of guanine hydrobromide monohydrate, in which the guanine base is protonated at N7, were X-irradiated at 8 and 65 K. Using K-band ESR, ENDOR, and field-swept-ENDOR (FSE) techniques, the crystals were studied between 8 K and room temperature. There was evidence for five different radicals, RI-RV, immedi...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::This study was designed to determine changes in cell numbers, proliferation (using Ki-67) and EGFR expression in mouse bladder urothelium during the early and late radiation response. Groups of mice were irradiated with a single dose of 20 Gy and assayed 0-360 days later. Urothelial cells were counted. After immunohis...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1759.1
更新日期:2010-04-01 00:00:00
abstract::A nested case-control study was conducted among workers at five U.S. nuclear facilities to evaluate leukemia mortality risk (excluding chronic lymphocytic) from ionizing radiation using worksite doses and adjusting for potential confounding. Conditional logistic regression was used to estimate the relative risk (RR) o...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr0724.1
更新日期:2007-02-01 00:00:00
abstract::Previous work showed that treatment of plateau-phase Chinese hamster ovary cells with the radiomimetic double-strand cleaving agent bleomycin induced very small deletions as well as interchromosomal reciprocal translocations, both of which could be ascribed to errors in end joining of DNA double-strand breaks. In an a...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)158[0327:bstsbd]2.0.co;2
更新日期:2002-09-01 00:00:00
abstract::The increasingly wider use of microdosimetry in fields other than pure radiobiological research, especially in "practical" domains such as radiation protection and medical physics, has created a number of new practitioners who are not fully acquainted with many of the experimental pitfalls which beset the aspiring mic...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::Deep-space travel presents risks of exposure to ionizing radiation composed of a spectrum of low-fluence protons (1H) and high-charge and energy (HZE) iron nuclei (e.g., 56Fe). When exposed to galactic cosmic rays, each cell in the body may be traversed by 1H every 3-4 days and HZE nuclei every 3-4 months. The effects...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14667.1
更新日期:2017-08-01 00:00:00
abstract::Tumor radiotherapy with large-field irradiation results in an increase of p53-dependent apoptosis of the radiosensitive hematopoietic stem cells. Proapoptotic PUMA is a transcriptional target of p53. Thus suppression of PUMA expression by gene therapy with the transcription repressor SNAI2 as transgene might be a pote...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1952.1
更新日期:2010-05-01 00:00:00
abstract::To investigate whether exposure to atomic bomb radiation altered the prevalence of hepatitis C virus (HCV) infection or accelerated the progress toward chronic hepatitis after HCV infection, the seropositivity of antibody to hepatitis C virus (anti-HCV) was determined for 6,121 participants in the Adult Health Study o...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)154[0012:poahcv]2.0.co;2
更新日期:2000-07-01 00:00:00
abstract::To approach treatment of micrometastases of steroid receptor-rich cancers using estrogen receptor-directed therapy with Auger electrons, multicellular spheroids of the estrogen receptor-rich human breast cancer cell line, MCF-7, were prepared and exposed to a range of concentrations of an Auger electron-emitting estro...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Proton exposure induces mutations and cancer, which are presumably linked. Because protons are abundant in the space environment and significant uncertainties exist for the effects of space travel on human health, the purpose of this study was to identify the types of mutations induced by exposure of mammalian cells t...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR3174.1
更新日期:2013-05-01 00:00:00
abstract::Currently, there is a serious absence of pharmaceutically attractive small molecules that mitigate the lethal effects of an accidental or intentional public exposure to toxic doses of ionizing radiation. Moreover, cellular systems that emulate the radiobiologically relevant cell populations and that are suitable for h...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr2810.1
更新日期:2012-09-01 00:00:00
abstract::Dose assessment after radiological disasters is imperative to decrease mortality through rationally directed medical intervention. Our goal was to identify biomarkers capable of qualitative (nonirradiated/irradiated) and/or quantitative (dose) assessment of radiation exposure. Using real-time quantitative PCR, biodosi...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2419.1
更新日期:2011-07-01 00:00:00
abstract::We have previously used NotI as the marker enzyme (recognizing GCGGCCGC) in a genome scanning approach for detection of mutations induced in mouse spermatogonia and estimated the mutation induction rate as about 0.7 x 10(-5) per locus per Gy. To see whether different parts of the genome have different sensitivities fo...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0926.1
更新日期:2007-08-01 00:00:00
abstract::In this study, we assessed the efficacy of unilamellar 110-nm liposomes encapsulating the chelating agent diethylenetriaminepentaacetic acid (DTPA) in plutonium-exposed rats. Rats were contaminated by intravenous administration of the soluble citrate form of plutonium. The comparative effects of liposomal and free DTP...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14968.1
更新日期:2018-05-01 00:00:00
abstract::Experimental data from laboratory animals indicate that the same extent of DNA damage or the same mutations in oncogenes and tumor suppressor genes in different hosts result in widely differing cancer development because of numerous polymorphic tumor susceptibility genes. Similarly, recent epidemiological data indicat...
journal_title:Radiation research
pub_type: 杂志文章,评审
doi:10.1667/rr3333.1
更新日期:2005-10-01 00:00:00
abstract::To study the acquired radioresistance of tumor cells, a model system of two cell lines, Djungarian hamster fibroblasts (DH-TK-) and their radioresistant progeny, was established. The progeny of irradiated cells were isolated by treating the parental cell monolayer with a single dose of 20 Gy (PIC-20). The genetic and ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3443.1
更新日期:2005-12-01 00:00:00
abstract::Hyperthermia sensitization or tolerance is subject to cellular events that may occur at membrane, nuclear, and cytoplasmic sites. We have studied the effects of elevated temperatures on the oxidative-reductive state of the cell by measuring and altering glutathione (GSH) concentrations. GSH plays a pivotal role in mai...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::Radiotherapy is one of the major treatment modalities for the management of various cancers, however, it is limited by the severe side effects and complications experienced by some patients. Nicaraven, a chemically synthesized hydroxyl radical-specific scavenger, has been shown to protect normal tissues from radiation...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14614.1
更新日期:2017-02-22 00:00:00
abstract::While the link between risk of leukemia and acute radiation exposure is well established for large doses received acutely, uncertainty remains around the translation of these risk estimates to occupational exposure scenarios where the doses are low and accumulated over time, possibly over many years. We present leukem...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR15358.1
更新日期:2019-11-01 00:00:00
abstract::Our data (Reddy et al., Radiat. Res. 141, 252-258, 1995) on the kinetics of the repair of potentially lethal damage in log-phase Chinese hamster V79 cells are used to test some predictions which arise from the different assumptions of the repair-misrepair (RMR) (C. A. Tobias, Radiat. Res. 104, S77-S95, 1985), lethal-p...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00