A model relating cell survival to DNA fragment loss and unrepaired double-strand breaks.


:The model of radiation action that is presented relates the surviving fraction of irradiated cells to unrepaired DNA double-strand breaks (DSBs). The following assumptions are made in the model: (i) A DNA fragment created by the induced DSBs may move out of its chromosome (become lost), and the probability of that process depends on the fragment size. (ii) An irradiated cell will lose its proliferative capacity if it has an unrepaired DSB (including DNA fragments) at certain points in the cell cycle. Mathematical expressions of the model yield the dose and time dependencies of the surviving fraction, the number of unrepaired DSBs, and the number of prematurely condensed chromosome fragments. Radiobiological phenomena described include effects of low dose rate, delayed plating, hypertonic solution, araA, and high-LET radiation. The calculated dose dependence of the residual number of unrepaired DSBs for ataxia telangiectasia and normal fibroblast cells is very close to the experimentally obtained [M. N. Cornforth and J. S. Bedford, Radiat. Res. 111, 385-405 (1987)] total number of chromosomal aberrations. This leads to the conclusion that each unrepaired DSB becomes a chromosomal aberration. Analysis in terms of the model shows that the radiosensitivity of various cell lines is predominantly due to the different amounts of time available for DSB repair in these cells.


Radiat Res


Radiation research


Ostashevsky JY


Has Abstract


1989-06-01 00:00:00












  • Bystander cell death and stress response is inhibited by the radical scavenger α(1)-microglobulin in irradiated cell cultures.

    abstract::Alpha-particle irradiation of cells damages not only the irradiated cells but also nontargeted bystander cells. It has been proposed that the bystander effect is caused by oxidants and free radicals generated by the radiation. Recent studies have shown that α(1)-microglobulin protects against cell damage caused by oxi...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Olsson MG,Nilsson EJ,Rutardóttir S,Paczesny J,Pallon J,Akerström B

    更新日期:2010-11-01 00:00:00

  • Karyotypic instability and centrosome aberrations in the progeny of finite life-span human mammary epithelial cells exposed to sparsely or densely ionizing radiation.

    abstract::The human breast is sensitive to radiation carcinogenesis, and genomic instability occurs early in breast cancer development. This study tests the hypothesis that ionizing radiation elicits genomic instability in finite life-span human mammary epithelial cells (HMEC) and asks whether densely ionizing radiation is a mo...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Sudo H,Garbe J,Stampfer MR,Barcellos-Hoff MH,Kronenberg A

    更新日期:2008-07-01 00:00:00

  • Analysis of boron distribution in vivo for boron neutron capture therapy using two different boron compounds by secondary ion mass spectrometry.

    abstract::The efficiency of boron neutron capture therapy (BNCT) for malignant gliomas depends on the selective and absolute accumulation of (10)B atoms in tumor tissues. Only two boron compounds, BPA and BSH, currently can be used clinically. However, the detailed distributions of these compounds have not been determined. Here...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Yokoyama K,Miyatake S,Kajimoto Y,Kawabata S,Doi A,Yoshida T,Okabe M,Kirihata M,Ono K,Kuroiwa T

    更新日期:2007-01-01 00:00:00

  • Cellular and molecular mechanisms underlying oxygen-dependent radiosensitivity.

    abstract::Molecular oxygen has long been recognized as a powerful radiosensitizer that enhances the cell-killing efficiency of ionizing radiation. Radiosensitization by oxygen occurs at very low concentrations with the half-maximum radiosensitization at approximately 3 mmHg. However, robust hypoxia-induced signal transduction c...

    journal_title:Radiation research

    pub_type: 杂志文章,评审


    authors: Liu C,Lin Q,Yun Z

    更新日期:2015-05-01 00:00:00

  • Effects of protons and HZE particles on glutamate transport in astrocytes, neurons and mixed cultures.

    abstract::Radiation-induced neurotoxicity is a well-characterized phenomenon. However, the underlying mechanism of this toxicity is poorly understood. In the central nervous system (CNS), excitotoxic mechanisms are implicated in many neurodegenerative disease processes. Pivotal to the excitotoxic pathway is dysfunction of gluta...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Sanchez MC,Nelson GA,Green LM

    更新日期:2010-12-01 00:00:00

  • Extensive repair of DNA double-strand breaks in cells deficient in the DNA-PK-dependent pathway of NHEJ after exclusion of heat-labile sites.

    abstract::Abstract Double-strand breaks (DSBs) can be generated in the DNA of mammalian cells when heat-labile sites induced by ionizing radiation within a clustered DNA damage site are thermally transformed to single-strand breaks (SSBs) and combine with other SSBs or heat-labile sites in the opposite DNA strand. When this the...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Singh SK,Wu W,Wu W,Wang M,Iliakis G

    更新日期:2009-08-01 00:00:00

  • Organ doses from radiation therapy in atomic bomb survivors.

    abstract::Previous surveys of radiation therapy among the Life Span Study (LSS) population at the Radiation Effects Research Foundation (RERF) revealed that 1,670 (1.4%) of the LSS participants received radiation treatments before 1984. The data on therapeutic radiation doses are indispensable for studying the relationship betw...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Kato K,Antoku S,Kodama K,Kawamura S,Fujita Y,Komatsu K,Awa AA

    更新日期:2001-06-01 00:00:00

  • An attempt to stimulate mitosis in Saccharomyces cerevisiae with the ultraviolet luminescence from exponential phase cultures of this yeast.

    abstract::Neither cell division nor growth of Saccharomyces cerevisiae were stimulated by the ultraviolet luminescence produced by adjacent exponential phase cultures of the yeast. The study included experiments in which the inocula (density = 5 X 10(7) cells cm-3) were irradiated and in which lag phase cultures (densities = 1 ...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Quickenden TI,Tilbury RN

    更新日期:1985-05-01 00:00:00

  • Biological imaging of heavy charged-particle tracks.

    abstract::The immunocytochemical response to DNA damage induced by low-energy bismuth and carbon ions was investigated in normal human fibroblasts. Inside the nuclei, the traversing charged particles lead to the accumulation of proteins related to DNA lesions and repair along the ion trajectories. Irradiation under a standard g...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Jakob B,Scholz M,Taucher-Scholz G

    更新日期:2003-05-01 00:00:00

  • Delayed Growth Suppression and Radioresistance Induced by Long-Term Continuous Gamma Irradiation.

    abstract::Biological response to ionizing radiation depends not only on the type of radiation and dose, but also on the duration and dose rate of treatment. For a given radiation dose, the biological response may differ based on duration and dose rate. We studied the properties of two human cell lines, M059K glioma and U2OS ost...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Nakajima H,Furukawa C,Chang YC,Ogata H,Magae J

    更新日期:2017-08-01 00:00:00

  • Radiobiological intercomparison of p(45)+Be and p(65)+Be neutron beams for lung tolerance in mice after single and fractionated irradiation.

    abstract::The lung tolerance in mice after single and fractionated irradiations with p(45)+Be and p(65)+Be neutrons produced at the isochronous cyclotron "CYCLONE" of Louvain-la-Neuve (Belgium) was studied. Cobalt-60 gamma rays were used for control irradiations. The end point was the dose which was lethal to 50% of the mice by...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Grégoire V,Beauduin M,Gueulette J,De Coster BM,Octave-Prignot M,Vynckier S,Wambersie A

    更新日期:1993-01-01 00:00:00

  • Biophysical modeling of fragment length distributions of DNA plasmids after X and heavy-ion irradiation analyzed by atomic force microscopy.

    abstract::The investigation of fragment length distributions of plasmid DNA gives insight into the influence of localized energy distribution on the induction of DNA damage, particularly the clustering of double-strand breaks. We present an approach that determines the fragment length distributions of plasmid DNA after heavy-io...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Elsässer T,Brons S,Psonka K,Scholz M,Gudowska-Nowak E,Taucher-Scholz G

    更新日期:2008-06-01 00:00:00

  • Sex-Specific Effects of a Wartime-Like Radiation Exposure on Cognitive Function.

    abstract::Evaluating the risk for central nervous system (CNS) effects after whole-body or partial-body irradiation presents challenges due in part to the varied exposure scenarios in the context of occupational, accidental or wartime releases. Risk estimations are further complicated by the fact that robust changes in brain fu...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Baddour AAD,Apodaca LA,Alikhani L,Lu C,Minasyan H,Batra RS,Acharya MM,Baulch JE

    更新日期:2020-01-01 00:00:00

  • Lack of behavioral effects in non-human primates after exposure to ultrawideband electromagnetic radiation in the microwave frequency range.

    abstract::The effect of acute exposure to ultrawideband (UWB) electromagnetic radiation on the Primate Equilibrium Platform (PEP) task, where the monkey's task is to manipulate a joystick control to compensate for the random perturbations in the pitch plane that are generated by a computer at unpredictable intervals, was examin...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Sherry CJ,Blick DW,Walters TJ,Brown GC,Murphy MR

    更新日期:1995-07-01 00:00:00

  • Eosinophil-Associated Gene Pathways but not Eosinophil Numbers are Differentially Regulated between Synchrotron Microbeam Radiation Treatment and Synchrotron Broad-Beam Treatment by 48 Hours Postirradiation.

    abstract::Synchrotron microbeam radiation treatment (MRT) is a preclinical radiotherapy technique with considerable clinical promise, although some of the underlying radiobiology of MRT is still not well understood. In recently reported studies, it has been suggested that MRT elicits a different tumor immune profile compared to...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Ibahim MJ,Yang Y,Crosbie JC,Stevenson A,Cann L,Paiva P,Rogers PA

    更新日期:2016-01-01 00:00:00

  • Toxicity of DNA-incorporated iodine-125: quantifying the direct and indirect effects.

    abstract::To understand the biophysical mechanism(s) underlying the induction of cell death by the decay of the Auger electron emitter iodine-125 in DNA, Chinese hamster V79 lung fibroblasts were labeled with 5-[(125)I]iodo-2'-deoxyuridine ((125)IdU) for two doubling times and frozen and stored at -135 degrees C in the presence...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Walicka MA,Ding Y,Adelstein SJ,Kassiss AI

    更新日期:2000-09-01 00:00:00

  • Hydrogen peroxide-induced base damage in deoxyribonucleic acid.

    abstract::Aqueous solutions of calf thymus deoxyribonucleic acid (DNA) were exposed to hydrogen peroxide in the presence of air. Base products formed in DNA were identified and quantitated following acid hydrolysis and trimethylsilylation using gas chromatography-mass spectrometry. The yields of these products were dependent up...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Blakely WF,Fuciarelli AF,Wegher BJ,Dizdaroglu M

    更新日期:1990-03-01 00:00:00

  • Radiomodfication of xanthine oxidoreductase system in the liver of mice by phenylmethylsulfonyl fluoride and dithiothreitol.

    abstract::The widely distributed xanthine oxidoreductase (XOR) system has been shown to be modulated upon exposure of animals to ionizing radiation through the conversion of xanthine dehydrogenase (XDH) into xanthine oxidase (XO). In the present work, radiomodification of the XOR system by phenylmethylsulfonyl fluoride (PMSF) a...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Srivastava M,Kale RK

    更新日期:2000-07-01 00:00:00

  • Uptake of tritiated thymidine in the root tip meristem of Vicia faba (L).

    abstract::The use of tritium-labeled thymidine (3H-TdR) in biological research made it necessary to develop a quick and accurate method for determination of tritium activity in tissue. After 3H-TdR incorporation into the root tip meristem of Vicia faba, total 3H activity as well as 3H-DNA activity was measured by liquid scintil...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Scheuermann W,Tägder K

    更新日期:1968-12-01 00:00:00

  • Dilution of irradiated cell conditioned medium and the bystander effect.

    abstract::While nontargeted and low-dose effects such as the bystander effect are now accepted, the mechanisms underlying the response have yet to be elucidated. It has been shown that the transfer of irradiated cell conditioned medium (ICCM) can kill cells that are not directly irradiated; however, to date the effect of ICCM c...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Ryan LA,Smith RW,Seymour CB,Mothersill CE

    更新日期:2008-02-01 00:00:00

  • Hyperthermic sensitivity and growth stage in Escherichia coli.

    abstract::Hyperthermic sensitivities of Escherichia coli B/r and Bs-1 were determined for lag-, midlog-, and stationary-phase cells at 47, 48, and 49 degrees C. In both strains midlog-phase cells were strikingly more heat sensitive (100-fold greater killing after 4 h at 48 degrees C) than stationary-phase cells, with intermedia...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Yatvin MB,Gipp JJ,Klessig DR,Dennis WH

    更新日期:1986-04-01 00:00:00

  • Radiation-induced alterations in mitochondria of the rat heart.

    abstract::Radiation therapy for the treatment of thoracic cancers may be associated with radiation-induced heart disease (RIHD), especially in long-term cancer survivors. Mechanisms by which radiation causes heart disease are largely unknown. To identify potential long-term contributions of mitochondria in the development of ra...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Sridharan V,Aykin-Burns N,Tripathi P,Krager KJ,Sharma SK,Moros EG,Corry PM,Nowak G,Hauer-Jensen M,Boerma M

    更新日期:2014-03-01 00:00:00

  • GS-nitroxide (JP4-039)-mediated radioprotection of human Fanconi anemia cell lines.

    abstract::Fanconi anemia (FA) is an inherited disorder characterized by defective DNA repair and cellular sensitivity to DNA crosslinking agents. Clinically, FA is associated with high risk for marrow failure, leukemia and head and neck squamous cell carcinoma (HNSCC). Radiosensitivity in FA patients compromises the use of tota...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Bernard ME,Kim H,Berhane H,Epperly MW,Franicola D,Zhang X,Houghton F,Shields D,Wang H,Bakkenist CJ,Frantz MC,Forbeck EM,Goff JP,Wipf P,Greenberger JS

    更新日期:2011-11-01 00:00:00

  • Inhibition of ABL tyrosine kinase potentiates radiation-induced terminal growth arrest in anaplastic thyroid cancer cells.

    abstract::Gleevec, a selective tyrosine kinase inhibitor, retarded the growth of anaplastic thyroid cancer cell lines in vitro and in vivo through selective inhibition of ABL tyrosine kinase activity. In the present study, we investigated the ability of Gleevec to modulate the in vitro and in vivo radiation response of anaplast...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Podtcheko A,Ohtsuru A,Namba H,Saenko V,Starenki D,Palona I,Sedliarou I,Rogounovitch T,Yamashita S

    更新日期:2006-01-01 00:00:00

  • Mitochondrial complex II dysfunction can contribute significantly to genomic instability after exposure to ionizing radiation.

    abstract::Ionizing radiation induces chronic metabolic oxidative stress and a mutator phenotype in hamster fibroblasts that is mediated by H(2)O(2), but the intracellular source of H(2)O(2) is not well defined. To determine the role of mitochondria in the radiation-induced mutator phenotype, end points of mitochondrial function...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Dayal D,Martin SM,Owens KM,Aykin-Burns N,Zhu Y,Boominathan A,Pain D,Limoli CL,Goswami PC,Domann FE,Spitz DR

    更新日期:2009-12-01 00:00:00

  • Effects of X irradiation on angiogenesis.

    abstract::We have evaluated the effect of X irradiation on the mesenchymal tissue growth (blood capillaries and stromal cells) in an angiogenesis system in the mouse. This was accomplished by implanting a polyvinyl alcohol sponge disc in the subcutis of the thorax, and quantifying the extent of growth reduction of capillaries a...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Prionas SD,Kowalski J,Fajardo LF,Kaplan I,Kwan HH,Allison AC

    更新日期:1990-10-01 00:00:00

  • Ionizing radiation alters neuronal excitability in hippocampal slices of the guinea pig.

    abstract::To investigate the effects of ionizing radiation on an isolated neuronal network without complicating systemic factors, slices of hippocampus from the guinea pig were isolated and studied in vitro. Slices were irradiated with a 60Co source and compared to paired, sham-irradiated controls. Electrophysiological activity...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Tolliver JM,Pellmar TC

    更新日期:1987-12-01 00:00:00

  • Antimutagenicity of WR-1065 in L5178Y cells exposed to accelerated (56)Fe ions.

    abstract::The ability of the aminothiol WR-1065 [N-(2-mercaptoethyl)-1,3-diaminopropane] to protect L5178Y (LY) cells against the cytotoxic and mutagenic effects of exposure to accelerated (56)Fe ions (1.08 GeV/nucleon) was determined. It was found that while WR-1065 reduced the mutagenicity in both cell lines when it was prese...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Evans HH,Evans TE,Horng MF

    更新日期:2002-07-01 00:00:00

  • (41)Ca in tooth enamel. Part I: a biological signature of neutron exposure in atomic bomb survivors.

    abstract::The detection of (41)Ca atoms in tooth enamel using accelerator mass spectrometry is suggested as a method capable of reconstructing thermal neutron exposures from atomic bomb survivors in Hiroshima and Nagasaki. In general, (41)Ca atoms are produced via thermal neutron capture by stable (40)Ca. Thus any (41)Ca atoms ...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Wallner A,Rühm W,Rugel G,Nakamura N,Arazi A,Faestermann T,Knie K,Maier HJ,Korschinek G

    更新日期:2010-08-01 00:00:00

  • Effect of mixed ligand complex therapy on the retention of 95Nb and 144Ce in mice.

    abstract::We have examined in mice the effects of mixed ligand treatments with desferrioxamine B (DFOA), Na3Ca-diethylenetriamine pentaacetic acid (DTPA), and DL-penicillamine (PA) on the retention of a mixture of 95Nb and 144Ce. The results show that 95Nb + 144Ce could be mobilized effectively by simultaneous application of sp...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Gachályi A,Naményi J,Szegedi I,Varga PL

    更新日期:1989-10-01 00:00:00