Abstract：
:Cells plated immediately after irradiation on nutrient agar (immediate plating) exhibit a lower survival than cells which are kept under nongrowth conditions before plating (delayed plating). The difference between the survival curves obtained after immediate plating and delayed plating is considered to exhibit the cell's capacity to repair potentially lethal damage. In yeast evidence has been presented previously for the DNA double-strand break (DSB) as the molecular lesion involved in the repair of potentially lethal damage observed at the cellular level. Radiation-induced DSB are repaired in cells plated on nutrient agar, i.e., under growth conditions, as well as in cells kept under nongrowth conditions. In this paper DSB repair under growth and nongrowth conditions is studied with the help of the yeast mutant rad54-3 which is temperature conditional for DSB repair. It is shown that the extent of repair of potentially lethal damage can be varied by shifting the relative fractions of repair of DSB under growth conditions versus nongrowth conditions. Repair of DSB in cells plated on nutrient agar is promoted when glucose is substituted by Na-succinate as an energy source. As a result the immediate plating survival curve approaches the delayed plating survival curve, thus reducing the operationally defined repair of potentially lethal damage. We show that this reduced potentially lethal damage repair is caused, however, by a higher amount of DSB repair in cells immediately plated on succinate agar as compared to glucose agar.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Frankenberg-Schwager M,Frankenberg D,Harbich Rsubject
Has Abstractpub_date
1987-08-01 00:00:00pages
192-200issue
2eissn
0033-7587issn
1938-5404journal_volume
111pub_type
杂志文章abstract：:After DNA damage, caspases cleave and activate proteins involved in cell death by apoptosis but also cleave and inactivate proteins implicated in DNA repair. Here we report a rapid onset of Rad51 cleavage by caspase 3 in BRCA2-defective mouse and human cells. This rapid cleavage was reduced markedly by transfer of ful...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1129.1
更新日期：2008-05-01 00:00:00
abstract：:The efficiency of boron neutron capture therapy (BNCT) for malignant gliomas depends on the selective and absolute accumulation of (10)B atoms in tumor tissues. Only two boron compounds, BPA and BSH, currently can be used clinically. However, the detailed distributions of these compounds have not been determined. Here...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0501.1
更新日期：2007-01-01 00:00:00
abstract：:A standardized dichlorofluorescin (DCF) fluorometric assay capable of measuring radiation-induced oxidative stress was used to determine the effectiveness of protons and high-mass, high-atomic number (Z) and high-energy (HZE) particles to produce oxidative stress in vitro. Protons were found to be about equally as eff...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2005)163[0364:doosib]2.0.co;2
更新日期：2005-04-01 00:00:00
abstract：:We have evaluated the effect of X irradiation on the mesenchymal tissue growth (blood capillaries and stromal cells) in an angiogenesis system in the mouse. This was accomplished by implanting a polyvinyl alcohol sponge disc in the subcutis of the thorax, and quantifying the extent of growth reduction of capillaries a...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:We recently reported that daily dietary supplementation with 100 µg selenium (a dose exceeding a rat's nutritional requirement by about 33-fold) initiated immediately after total-body irradiation (TBI) and maintained for 21 weeks mitigates radiation nephropathy in a rat model as indicated by blood urea nitrogen (BUN) ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2456.1
更新日期：2011-09-01 00:00:00
abstract：:Radiation-induced neurotoxicity is a well-characterized phenomenon. However, the underlying mechanism of this toxicity is poorly understood. In the central nervous system (CNS), excitotoxic mechanisms are implicated in many neurodegenerative disease processes. Pivotal to the excitotoxic pathway is dysfunction of gluta...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2106.1
更新日期：2010-12-01 00:00:00
abstract：:The cellular sites for damage in mammalian cells caused by 193-nm radiation from an argon fluoride excimer laser were investigated. The ability of Chinese hamster ovary cells to reduce a tetrazolium dye (MTT) was decreased to 37% of unirradiated control by 2.5 x 10(3) J/m2 of 193-nm radiation when measured either 4 or...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:This study investigated whether the clonogenic ability of tumor cells to establish subcutaneous tumors and the tumor-associated macrophage (TAM) content correlate with the development and extent of the tumor bed effect (TBE). Ten tumors, five sarcomas and five carcinomas, syngeneic to C3Hf/Kam mice were used. Tumors w...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Many studies have demonstrated the effect of oncogene transfection on the radiation sensitivity of primary rat embryo fibroblasts. Our results indicate that transformation by H-ras plus v-myc oncogenes confers radiation resistance to a much greater extent than transformation by either gene alone. We have further shown...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：
更新日期：1994-04-01 00:00:00
abstract：:Cell-cell contact is thought to be critically involved in mechanisms leading to radioresistance. Here, we assessed the influence of confluent compared to subconfluent cell culture conditions and the radiosensitizing ability of E-cadherin inhibition alone or in combination with C225-mediated EGFR inhibition in human sq...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2855.1
更新日期：2012-09-01 00:00:00
abstract：:The radiation environment in low-Earth orbit is a complex mixture of galactic cosmic radiation, particles of trapped belts and secondary particles generated in both the spacecraft and Earth's atmosphere. Infrequently, solar energetic particles are injected into the Earth's magnetosphere and can penetrate into low-Eart...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：
更新日期：1997-11-01 00:00:00
abstract：:Experimental measurements of the radial restricted linear energy transfer (LETr) for alpha beams of 18.3 MeV/n in tissue-equivalent gas were presented. The radial dose distribution for the alpha beam was deduced from the restricted LET measurements. A differential W value for the alpha particle in the tissue-equivalen...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
abstract：:A total of 155 primary bone sarcomas were found in 131 of the 246 beagles injected with 226Ra and 5 primary bone sarcomas were found in 4 of the 158 unexposed controls. Of these 155 bone sarcomas, 146 (94%) were osteosarcomas and 9 were non-osteosarcomas. An additional 31 primary bone sarcomas (28 osteosarcomas) devel...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:The effects of bromodeoxyuridine (BrdUrd) substitution for thymidine on gamma-ray-induced strand breakage were determined in single- and double-stranded oligonucleotides and double-stranded oligonucleotides containing a mismatched bubble region. BrdUrd does not sensitize complementary double-stranded DNA to gamma-ray-...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3267
更新日期：2004-12-01 00:00:00
abstract：:Nearly 2% of the male population of Estonia aged 20-39 years were sent to Chernobyl to assist in the cleanup activities after the reactor accident. A cohort of 4,833 cleanup workers was assembled based on multiple and independent sources of information. Information obtained from 3,704 responses to a detailed questionn...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：:Braun, R. D., Lanzen, J. L., Turnage, J. A., Rosner, G. and Dewhirst, M. W. Effects of the Interaction between Carbogen and Nicotinamide on R3230 Ac Tumor Blood Flow in Fischer 344 Rats. Radiat. Res. 155, 724-733 (2001). The purpose of this study was to determine whether there are interactions between carbogen breathi...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0724:eotibc]2.0.co;2
更新日期：2001-05-01 00:00:00
abstract：:We have examined the role of natural killer (NK) cells in situ in the recovery of marrow hematopoiesis in B6D2F1 mice receiving various doses of total-body irradiation (TBI) as a well-characterized model for treatment-induced myelosuppression. Applying an in situ cytotoxic approach for ablating NK 1.1 cells, we have d...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:Inhalation and ingestion of 137Cs and other long-lived radionuclides can occur after large-scale accidental or malicious radioactive contamination incidents, resulting in a complex temporal pattern of radiation dose/dose rate, influenced by radionuclide pharmacokinetics and chemical properties. High-throughput radiati...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RADE-20-00042.1
更新日期：2020-10-16 00:00:00
abstract：:Well-defined protocols and quality management standards are indispensable for biological dosimetry laboratories. Participation in periodic proficiency testing by interlaboratory comparisons is also required. This harmonization is essential if a cooperative network is used to respond to a mass casualty event. Here we p...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2425.1
更新日期：2011-05-01 00:00:00
abstract：:The lineal energy distribution and several other dosimetric parameters were measured for the neutrons emitted from a replica of the Hiroshima bomb to determine their usefulness in biological experiments designed to estimate the effectiveness of actual Hiroshima neutrons. The "Little-Boy" replica (LBR) was constructed ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:Ion microscopy was used for subcellular quantitative imaging of the isotopes 10B and 11B in the same cell to evaluate boron delivery using a mixture of two neutron capture therapy drugs, p-boronophenylalanine-fructose (BPA-F) and sodium borocaptate (BSH). The application of 10B-labeled BPA-F and 11B-labeled BSH allowe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0700:qssims]2.0.co;2
更新日期：2002-06-01 00:00:00
abstract：:Relatively few data are available on the prevalence of hyperthyroidism (TSH concentrations of <0.3 mIU/liter, with normal or elevated concentrations of free T4) in individuals exposed to radioiodines at low levels. The accident at the Chornobyl (Chernobyl) nuclear plant in Ukraine on April 26, 1986 exposed large numbe...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2003.1
更新日期：2010-12-01 00:00:00
abstract：:Mathematical analysis of cell survival allows parameter estimation for radiobiological models and selection of an appropriate model. To our knowledge, no rigorous comparisons on the accuracy of various methods used for such analysis have been performed. In this study we compared three methods: 1. maximization of binom...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14195.1
更新日期：2016-03-01 00:00:00
abstract：:Ionizing radiations induce mutations which can be detected both in coding sequences (Hprt locus) by measuring the frequency of 6-thioguanine-resistant cells and in minisatellite sequences by DNA fingerprint analysis. We analyzed the effects of irradiation with low-energy protons (31 keV/pm) and, for comparison, with u...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Data are presented for a subset of lymphocytes characterized by FISH as missing painted chromosomal material. These lymphocytes occur in both control and irradiated subjects. These cells have a much greater frequency of one-way translocations than cells in which all of the painted chromosomal material is present. Thei...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0469:cmfbdb]2.0.co;2
更新日期：2002-04-01 00:00:00
abstract：:Rat females were exposed to a single 4.0-Gy gamma-ray dose and treated postradiation with a mixture of ATP-AET-mexamine at daily doses of 24, 12, and 3 mg/kg body wt, respectively, in drinking water throughout the period of their survival. With the radiation dose used, life shortening appeared primarily attributable t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Prior to whole-liver irradiation (0, 15, 25 Gy) rats were not treated, placed on a protein-deficient diet for 2 weeks, administered cyclophosphamide, and/or depleted of intracellular glutathione by injection with buthionine sulfoximide and diethyl maleate. At various times (0 to 84 days) after 0- and 25-Gy liver irrad...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Little computational or experimental information is available on site-specific hydroxyl attack probabilities to DNA. In this study, an atomistic stochastic model of OH radical reactions with DNA was developed to compute relative OH attack probabilities at individual deoxyribose hydrogen atoms. A model of the self-comp...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0038:ssoatt]2.0.co;2
更新日期：2002-01-01 00:00:00
abstract：:The electroporation of restriction enzymes into mammalian cells results in DNA double-strand breaks that can lead to chromosome aberrations. Four chemicals known to interfere with cellular responses to DNA damage were investigated for their effects on chromosome aberrations induced by AluI and Sau3AI; in addition, the...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Cells from patients with ataxia telangiectasia (AT) are abnormal in their response to irradiation as judged by clonogenic survival and accumulation in G2 phase. The relationship of the results of these two assays, however, is still a matter of controversy. Flow cytometry was used to measure the distribution of cells i...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
