Abstract：
:To study the acquired radioresistance of tumor cells, a model system of two cell lines, Djungarian hamster fibroblasts (DH-TK-) and their radioresistant progeny, was established. The progeny of irradiated cells were isolated by treating the parental cell monolayer with a single dose of 20 Gy (PIC-20). The genetic and morphological features, clonogenic ability, radiosensitivity, cell growth kinetics, ability to grow in methylcellulose, and tumorigenicity of these cell lines were compared. The plating efficiency of PIC-20 cells exceeded that of DH-TK- cells. The progeny of irradiated cells were more radioresistant than parental cells. The average D0 for PIC-20 cells was 7.4 +/- 0.2 Gy, which is three times higher than that for parental cells (2.5 +/- 0.1 Gy). Progeny cell survival in methylcellulose after irradiation with a dose of 10 Gy was 15 times higher than that of DH-TK- cells. In contrast to parental cells, the progeny of irradiated cells showed fast and effective repopulation after irradiation with doses of 12.5 and 15 Gy. The tumor formation ability of irradiated progeny cells was higher than that of parental cells; after 15 Gy irradiation, PIC-20 cells produced tumors as large as unirradiated progeny of irradiated cells, whereas the tumor development of DH-TK- cells diminished by 70%. High radioresistance of progeny of irradiated cells was reproduced during the long period of cultivation (more than 80 passages). The stability of the radioresistant phenotype of PIC-20 cells allows us to investigate the possible mechanisms of acquired tumor radioresistance.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Tyrsina EG,Slanina SV,Kakpakova ES,Kalendo GS,Kan NG,Tyrsin OY,Ryskov APdoi
10.1667/rr3443.1subject
Has Abstractpub_date
2005-12-01 00:00:00pages
745-54issue
6eissn
0033-7587issn
1938-5404pii
RR3443journal_volume
164pub_type
杂志文章abstract：:Data are presented for a subset of lymphocytes characterized by FISH as missing painted chromosomal material. These lymphocytes occur in both control and irradiated subjects. These cells have a much greater frequency of one-way translocations than cells in which all of the painted chromosomal material is present. Thei...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0469:cmfbdb]2.0.co;2
更新日期：2002-04-01 00:00:00
abstract：:Radiation-induced complications of the respiratory system are a common side effect of thoracic radiotherapy with no viable treatment option. Here, we investigated the potential therapeutic effect of the orphan drug pirfenidone for treating radiation-induced pulmonary fibrosis. C57BL/6 mice received a single fraction o...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15017.1
更新日期：2018-10-01 00:00:00
abstract：:Single crystals of disodium beta-glycerophosphate pentahemihydrate (betaGP), Na2x(HO)CH2CH(PO(4)2-)CH2(OH)x5 1/2H2O, were X-irradiated at 77 and 280 K. EPR, ENDOR and FSE techniques were used to study the formation of free radicals in these irradiated crystals to characterize possible reaction mechanisms leading to de...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：:The threat of exposure to ionizing radiation from a nuclear reactor accident or deliberate terrorist actions is a significant public health concern. The lung is particularly susceptible to radiation-induced injury from external sources or inhalation of radioactive particles from radioactive fallout. Radiation-induced ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14686.1
更新日期：2017-11-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:Breast tissue is very susceptible to radiation-induced carcinogenesis, and mammary stem/progenitor cells are potentially important targets of this. The mammary epithelium is maintained as two mostly independent lineages of luminal and basal cells. To elucidate their immediate radiation responses, we analyzed the mamma...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15566.1
更新日期：2020-07-08 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14653.1
更新日期：2018-01-01 00:00:00
abstract：:Sequential examinations were done on the pulmonary cytokinetics and pulmonary lesions in rats after inhalation exposure to (239)PuO(2) aerosols to investigate the pathogenesis of lung tumors. Total cell yields of lavaged bronchoalveolar cells as well as the estimated numbers of pulmonary alveolar macrophages were sign...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)154[0253:ppolti]2.0.co;2
更新日期：2000-09-01 00:00:00
abstract：:To characterize the differences in the radiosensitivity of individual populations of human hematopoietic stem/progenitor cells (HSPCs), we examined the relationship among cell surface antigens, clonogenic potential and radiation survival. The expressions of CD34, CD38, CD45RA, CD110 and Tie-2, early differentiation pa...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1839.1
更新日期：2010-02-01 00:00:00
abstract：:Vascular injury after radiation exposure contributes to multiple types of tissue injury through a cascade of events. Some of the earliest consequences of radiation damage include increased vascular permeability and promotion of inflammation, which is partially manifested by increased leukocyte-endothelial (L/E) intera...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14896.1
更新日期：2018-07-01 00:00:00
abstract：:At the Swiss Institute for Nuclear Research (SIN) cancer patients are irradiated with negatively charged pi mesons using a 60-beam medical pion generator, the Piotron. A low-pressure tissue-equivalent proportional counter was used to measure absorbed dose and microdosimetric spectra. A method was developed to allow di...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Molecular imaging is an evolving science that is concerned with the development of novel imaging probes and biomarkers that can be used to non-invasively image molecular and cellular processes. This special issue approaches molecular imaging in the context of radiation research, focusing on biomarkers and imaging meth...
journal_title：Radiation research
pub_type：
doi：10.1667/rr2959.1
更新日期：2012-04-01 00:00:00
abstract：:The relationship between apoptosis induced by gamma radiation and glutathione in cells of two human cancer cell lines, HeLa from cervix carcinoma and MCF-7 from mammary carcinoma, was examined. MCF-7 cells appeared to be more radioresistant than HeLa cells, and radiation-induced apoptosis, which was monitored by asses...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0578.1
更新日期：2007-03-01 00:00:00
abstract：:The hearts of mature male rats of the Wistar and Sprague-Dawley strains were locally irradiated with single doses of 17.5 and 20.0 Gy of X rays, respectively. These two dose levels had previously been shown to result in a comparable latent period between irradiation and the death of rats of these two strains from card...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Cells plated immediately after irradiation on nutrient agar (immediate plating) exhibit a lower survival than cells which are kept under nongrowth conditions before plating (delayed plating). The difference between the survival curves obtained after immediate plating and delayed plating is considered to exhibit the ce...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:Rat females were exposed to a single 4.0-Gy gamma-ray dose and treated postradiation with a mixture of ATP-AET-mexamine at daily doses of 24, 12, and 3 mg/kg body wt, respectively, in drinking water throughout the period of their survival. With the radiation dose used, life shortening appeared primarily attributable t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:The ability of the aminothiol WR-1065 [N-(2-mercaptoethyl)-1,3-diaminopropane] to protect L5178Y (LY) cells against the cytotoxic and mutagenic effects of exposure to accelerated (56)Fe ions (1.08 GeV/nucleon) was determined. It was found that while WR-1065 reduced the mutagenicity in both cell lines when it was prese...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0110:aowilc]2.0.co;2
更新日期：2002-07-01 00:00:00
abstract：:Exposure of cells to heat induces thermotolerance, a transient resistance to subsequent heat challenges. It has been shown that thermotolerance is correlated in time with the enhanced synthesis of heat shock proteins. In this study, the association of induced heat shock proteins with various cellular fractions was inv...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:Dose-spread kernels can be used to calculate the dose distribution in a photon beam by convolving the kernel with the primary fluence distribution. The theoretical relationships between various types and components of dose-spread kernels relative to photon attenuation coefficients are explored. These relations can be ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:We have been investigating radiation-induced neoplastic transformants of C3H 10T1/2 mouse fibroblasts for evidence of heritable changes. C3H 10T1/2 cells were treated with 8 Gy X rays. After approximately 8 weeks of culture, type II/III foci were isolated from the monolayer using cloning rings. Cell lines developed fr...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Growth retardation due to exposure to the atomic bomb has been evaluated using repeated measurements of stature from 10 to 18 years of age. A highly significant growth retardation due to the DS86 uterine dose was observed in all trimesters combined and in the first and second trimesters of pregnancy. In the first trim...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:The statistical distribution of the number of ion pairs per ionizing event in a small volume simulating a tissue sphere was obtained by applying the Expectation-Maximization (EM) algorithm to experimental spectra measured by exposing a Rossi-type spherical proportional counter to gamma radiation. The normalized experi...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:The radiation risk of skin cancer by histological types has been evaluated in the atomic bomb survivors. We examined 80,158 of the 120,321 cohort members who had their radiation dose estimated by the latest dosimetry system (DS02). Potential skin tumors diagnosed from 1958 to 1996 were reviewed by a panel of pathologi...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13494.1
更新日期：2014-05-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:The current status of particle radiotherapy from a historical perspective is presented. This is done with a personal view and contains personal references and memories during the development of particle radiotherapy. The particles covered are fast neutrons, neutron capture therapy, protons, helium ions, pions and heav...
journal_title：Radiation research
pub_type： 历史文章,杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Pulse radiolysis and 60Co gamma radiolysis were used to study the effects of ionizing radiations on the activity of glutamate dehydrogenase. Hydroxyl radicals are considerably more effective than hydrated electrons in causing loss of enzymatic activity. Evidence is also presented that the free radical anions (SCN)-.2,...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Relationships based on the microdosimetric-kinetic model are presented that calculate the average number of lethal lesions, and the associated cell survival, produced in mammalian cells by exposure to protracted continuous irradiation by temporary and permanent implantation of radioactive sources. The influence of cel...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13558.1
更新日期：2014-07-01 00:00:00
abstract：:The increasing use of mobile phones by children and teenagers has raised concerns about their safety. Addressing such concerns is difficult, because no data are available on possible effects from long-term exposure to radiofrequency (RF) fields during the development of the nervous system. Possible morphological and f...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1002.1
更新日期：2007-10-01 00:00:00
abstract：:Chinese hamster ovary cells were irradiated with 250 kVp X rays and analyzed for the presence of DNA double-strand breaks using either polycarbonate filter elution or pulsed-field agarose gel electrophoresis at neutral pH. Reduction in DNA length detected by filter elution was produced as a nonlinear function of incre...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:If the limited life span of hematopoietic tissues in vitro is due to a finite proliferative capacity of individual stem cells, one might expect tissues of young donors to possess a greater proliferative capacity and to contain a larger population of primitive stem cells than those of older donors. To test this hypothe...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
