Application of a Novel Murine Ear Vein Model to Evaluate the Effects of a Vascular Radioprotectant on Radiation-Induced Vascular Permeability and Leukocyte Adhesion.

Abstract:

:Vascular injury after radiation exposure contributes to multiple types of tissue injury through a cascade of events. Some of the earliest consequences of radiation damage include increased vascular permeability and promotion of inflammation, which is partially manifested by increased leukocyte-endothelial (L/E) interactions. We describe herein a novel intravital imaging method to evaluate L/E interactions, as a function of shear stress, and vascular permeability at multiple time points after local irradiation to the ear. This model permitted analysis of quiescent vasculature that was not perturbed by any surgical manipulation prior to imaging. To evaluate the effects of radiation on vascular integrity, fluorescent dextran was injected intravenously and its extravasation in the extravascular space surrounding the ear vasculature was measured at days 3 and 7 after 6 Gy irradiation. The vascular permeability rate increased approximately twofold at both days 3 and 7 postirradiation ( P < 0.05). Leukocyte rolling, which is indicative of L/E interactions, was significantly increased in mice at 24 h postirradiation compared to that of nonirradiated mice. To assess our model, as a means for assessing vascular radioprotectants, we treated additional cohorts of mice with a thrombopoietin mimetic, TPOm (RWJ-800088). In addition to stimulating platelet formation, thrombopoietin can protect vasculature after several forms of injury. Thus, we hypothesized that TPOm would reduce vascular permeability and L/E adhesion after localized irradiation to the ear vasculature of mice. If TPOm reduced these consequences of radiation, it would validate the utility of our intravital imaging method. TPOm reduced radiation-induced vascular leakage to control levels at day 7. Furthermore, L/E cell interactions were also reduced in irradiated mice treated with TPOm, compared with mice receiving irradiation alone, particularly at high shear stress ( P = 0.03, Kruskal-Wallis). We conclude that the ear model is useful for monitoring quiescent normal tissue vascular injury after radiation exposure. Furthermore, the application of TPOm, for preventing early inflammatory response created by damage to vascular endothelium, suggests that this drug may prove useful in reducing toxicities from radiotherapy, which damage microvasculature that critically important to tissue function.

journal_name

Radiat Res

journal_title

Radiation research

authors

Ashcraft KA,Choudhury KR,Birer SR,Hendargo HC,Patel P,Eichenbaum G,Dewhirst MW

doi

10.1667/RR14896.1

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

12-21

issue

1

eissn

0033-7587

issn

1938-5404

journal_volume

190

pub_type

杂志文章
  • Quantitative subcellular secondary ion mass spectrometry (SIMS) imaging of boron-10 and boron-11 isotopes in the same cell delivered by two combined BNCT drugs: in vitro studies on human glioblastoma T98G cells.

    abstract::Ion microscopy was used for subcellular quantitative imaging of the isotopes 10B and 11B in the same cell to evaluate boron delivery using a mixture of two neutron capture therapy drugs, p-boronophenylalanine-fructose (BPA-F) and sodium borocaptate (BSH). The application of 10B-labeled BPA-F and 11B-labeled BSH allowe...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/0033-7587(2002)157[0700:qssims]2.0.co;2

    authors: Chandra S,Lorey II DR,Smith DR

    更新日期:2002-06-01 00:00:00

  • Binucleate cell formation correlates to loss of colony-forming ability in X-irradiated cultured mammalian cells.

    abstract::The relationship between binucleate cell formation and the loss of colony-forming ability was examined in several cultured mammalian cell lines irradiated with X rays. The maximum fraction of binucleate cells after X irradiation increased dose-dependently within the range in which reproductive cell death might predomi...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Sasaki H,Yoshinaga H,Kura S

    更新日期:1986-12-01 00:00:00

  • CBLB613: a TLR 2/6 agonist, natural lipopeptide of Mycoplasma arginini , as a novel radiation countermeasure.

    abstract::To date, there are no safe and effective drugs available for protection against ionizing radiation damage. Therefore, a great need exists to identify and develop non-toxic agents that will be useful as radioprotectors or postirradiation therapies under a variety of operational scenarios. We have developed a new pharma...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/rr2657.1

    authors: Singh VK,Ducey EJ,Fatanmi OO,Singh PK,Brown DS,Purmal A,Shakhova VV,Gudkov AV,Feinstein E,Shakhov A

    更新日期:2012-05-01 00:00:00

  • Recovery from effects of heat on DNA synthesis in Chinese hamster ovary cells.

    abstract::The hyperthermic inhibition of cellular DNA synthesis, i.e., reduction in replicon initiation and delay in DNA chain elongation, was previously postulated to be involved in the induction of chromosomal aberrations believed to be largely responsible for killing S-phase cells. Utilizing asynchronous Chinese hamster ovar...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Wong RS,Thompson LL,Dewey WC

    更新日期:1988-04-01 00:00:00

  • Effect of low-dose preirradiation on induction of the HSP70B-LacZ fusion gene in human cells treated with heat shock.

    abstract::We examined the effects of X irradiation on activation of the promoter of the human HSP70B gene, which is activated only after heat shock. The HSP70B promoter was ligated to the bacterial beta-galactosidase (beta-gal) gene, and the reporter plasmid was transfected into NCI-H1299 carcinoma cells. The expression of the ...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Suzuki K,Kodama S,Watanabe M

    更新日期:1998-02-01 00:00:00

  • Repression of multiple myeloma growth and preservation of bone with combined radiotherapy and anti-angiogenic agent.

    abstract::The effects of ionizing radiation, with or without the anti-angiogenic agent anginex (Ax), on multiple myeloma growth were tested in a SCID-rab mouse model. Mice carrying human multiple myeloma cell-containing pre-implanted bone grafts were treated weekly with various regimens for 8 weeks. Rapid multiple myeloma growt...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR1734.1

    authors: Jia D,Koonce NA,Halakatti R,Li X,Yaccoby S,Swain FL,Suva LJ,Hennings L,Berridge MS,Apana SM,Mayo K,Corry PM,Griffin RJ

    更新日期:2010-06-01 00:00:00

  • Intercellular communication is involved in the bystander regulation of gene expression in human cells exposed to very low fluences of alpha particles.

    abstract::We demonstrate by western analysis that the expression levels of TP53 (formerly known as p53), CDKN1A (formerly known as p21Waf1), CDC2 (formerly known as p34cdc2), CCNB1 (cyclin B1) and RAD51 are significantly modulated in confluent, density-inhibited human diploid cell populations exposed to doses where only a small...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Azzam EI,de Toledo SM,Gooding T,Little JB

    更新日期:1998-11-01 00:00:00

  • The influence of estrogen treatment on induction of mammary carcinoma in rats by single-dose gamma irradiation at different ages.

    abstract::The effect of age at exposure on induction of mammary carcinoma was studied in female rats of the inbred WAG/Rij strain that were treated with estrogen. Groups of 40 animals were exposed to a single total-body dose of 1 or 2 Gy of 137Cs gamma radiation at age 8, 10, 12, 15, 22, 36 or 64 weeks. Hormone levels in the an...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Bartstra RW,Bentvelzen PA,Zoetelief J,Mulder AH,Broerse JJ,van Bekkum DW

    更新日期:1998-10-01 00:00:00

  • Laboratory intercomparison of the dicentric chromosome analysis assay.

    abstract::The study design and obtained results represent an intercomparison of various laboratories performing dose assessment using the dicentric chromosome analysis (DCA) as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibrat...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR3235.1

    authors: Beinke C,Barnard S,Boulay-Greene H,De Amicis A,De Sanctis S,Herodin F,Jones A,Kulka U,Lista F,Lloyd D,Martigne P,Moquet J,Oestreicher U,Romm H,Rothkamm K,Valente M,Meineke V,Braselmann H,Abend M

    更新日期:2013-08-01 00:00:00

  • Organ doses from radiation therapy in atomic bomb survivors.

    abstract::Previous surveys of radiation therapy among the Life Span Study (LSS) population at the Radiation Effects Research Foundation (RERF) revealed that 1,670 (1.4%) of the LSS participants received radiation treatments before 1984. The data on therapeutic radiation doses are indispensable for studying the relationship betw...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/0033-7587(2001)155[0785:odfrti]2.0.co;2

    authors: Kato K,Antoku S,Kodama K,Kawamura S,Fujita Y,Komatsu K,Awa AA

    更新日期:2001-06-01 00:00:00

  • Comparison of Helzel and OxyLite systems in the measurements of tumor partial oxygen pressure (pO2).

    abstract::Wen, B., Urano, M., Humm, J. L., Seshan, V. E., Li, G. C. and Ling, C. C. Comparison of Helzel and OxyLite Systems in the Measurements of Tumor Partial Oxygen Pressure (pO(2)). Radiat. Res. 168, 67-75 (2008). It has been demonstrated in both experimental and human malignancies that hypoxic tumor cells are linked with ...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR0888.1

    authors: Wen B,Urano M,Humm JL,Seshan VE,Li GC,Ling CC

    更新日期:2008-01-01 00:00:00

  • A Comparison of Kinetic Photon Cell Survival Models.

    abstract::We report here on a qualitative and quantitative comparison of four kinetic photon cell survival models. The commonly used linear-quadratic model extended by a dose protraction factor, the lethal potentially lethal model, the repair misrepair model and the recently reported Giant LOop Binary LEsion (GLOBLE) model are ...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR13862.1

    authors: Herr L,Friedrich T,Durante M,Scholz M

    更新日期:2015-11-01 00:00:00

  • Choosing metaphases for biological dosimetry by fluorescence in situ hybridization (FISH).

    abstract::Data are presented for a subset of lymphocytes characterized by FISH as missing painted chromosomal material. These lymphocytes occur in both control and irradiated subjects. These cells have a much greater frequency of one-way translocations than cells in which all of the painted chromosomal material is present. Thei...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/0033-7587(2002)157[0469:cmfbdb]2.0.co;2

    authors: Edwards A,Maznik N,Moquet J,Hone P,Vinnikov V,Lloyd D,Cox R

    更新日期:2002-04-01 00:00:00

  • Comparison of gamma-ray-induced chromosome ring and inversion frequencies.

    abstract::A method was used to detect chromosome inversions as apparent or false sister chromatid exchanges (SCEs) in the first mitosis after gamma irradiation of human G0 cells. Dose-response relationships for small inversions have not been measured and reported previously, but it has been assumed that these are induced with a...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Mühlmann-Díaz MC,Bedford JS

    更新日期:1995-08-01 00:00:00

  • Karyotypic instability and centrosome aberrations in the progeny of finite life-span human mammary epithelial cells exposed to sparsely or densely ionizing radiation.

    abstract::The human breast is sensitive to radiation carcinogenesis, and genomic instability occurs early in breast cancer development. This study tests the hypothesis that ionizing radiation elicits genomic instability in finite life-span human mammary epithelial cells (HMEC) and asks whether densely ionizing radiation is a mo...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR1317.1

    authors: Sudo H,Garbe J,Stampfer MR,Barcellos-Hoff MH,Kronenberg A

    更新日期:2008-07-01 00:00:00

  • Postirradiation glucan administration enhances the radioprotective effects of WR-2721.

    abstract::Based on murine survival studies, endogenous hemopoietic spleen colony formation (E-CFU), and recovery of bone marrow and splenic granulocyte-macrophage colony-forming cells (GM-CFC), it was demonstrated that the postirradiation administration of glucan, an immunomodulator and hemopoietic stimulant, enhances the radio...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Patchen ML,MacVittie TJ,Jackson WE

    更新日期:1989-01-01 00:00:00

  • Nuclear protein following heat shock: protein removal kinetics and cell cycle rearrangements.

    abstract::Nuclear protein and DNA content of HeLa cells was determined as a function of time following hyperthermia by staining isolated nuclei with two fluorescent dyes: fluorescein isothiocyanate (FITC) for protein content and propidium iodide (PI) for DNA content. Bivariate FITC and PI histograms were obtained by flow cytome...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Roti Roti JL,Uygur N,Higashikubo R

    更新日期:1986-08-01 00:00:00

  • Inborn errors in metabolism and 4-boronophenylalanine-fructose-based boron neutron capture therapy.

    abstract::Infusions of boronophenylalanine-fructose complex (BPA-F), at doses up to 900 mg/kg of BPA and 860 mg/kg of fructose, have been used to deliver boron to cancer tissue for boron neutron capture therapy (BNCT). In patients with phenylketonuria (PKU), phenylalanine accumulates, which is harmful in the long run. PKU has b...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/rr3067

    authors: Laakso J,Ruokonen I,Lapatto R,Kallio M

    更新日期:2003-11-01 00:00:00

  • Human chromosome 8 (p12-->q22) complements radiosensitivity in the severe combined immune deficiency (SCID) mouse.

    abstract::The severe combined immune deficiency (SCID) mouse shows two kinds of phenotypic abnormalities, a high radiosensitivity and an abnormal immunoglobulin gene recombination. A genetic study has revealed that a mutation exists in chromosome 16. However, several attempts to isolate the gene responsible for these phenotypes...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Itoh M,Hamatani K,Komatsu K,Araki R,Takayama K,Abe M

    更新日期:1993-06-01 00:00:00

  • Identification of nitroxide radioprotectors.

    abstract::The nitroxide Tempol, a stable free radical, has recently been shown to protect mammalian cells against several forms of oxidative stress including radiation-induced cytotoxicity. To extend this observation, six additional water-soluble nitroxides with different structural features were evaluated for potential radiopr...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Hahn SM,Wilson L,Krishna CM,Liebmann J,DeGraff W,Gamson J,Samuni A,Venzon D,Mitchell JB

    更新日期:1992-10-01 00:00:00

  • Defects in the kinetics of the repair of DNA double-strand breaks and inhibition of DNA synthesis in the ataxia telangiectasia AT5BI-VA cell line: comparison to a corrected hybrid, atxbc.

    abstract::The nature of the primary biochemical defect in the human radiosensitive and cancer-prone syndrome, ataxia telangiectasia (AT), has remained obscure despite many efforts to elucidate it. In this study, AT complementation group D cells and a nearly isogenic corrected AT-hamster hybrid derivative have been analyzed for ...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Kysela BP,Lohrer H,Arrand JE

    更新日期:1995-12-01 00:00:00

  • Gene expression analysis in radiotherapy patients and C57BL/6 mice as a measure of exposure to ionizing radiation.

    abstract::Dose assessment after radiological disasters is imperative to decrease mortality through rationally directed medical intervention. Our goal was to identify biomarkers capable of qualitative (nonirradiated/irradiated) and/or quantitative (dose) assessment of radiation exposure. Using real-time quantitative PCR, biodosi...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR2419.1

    authors: Filiano AN,Fathallah-Shaykh HM,Fiveash J,Gage J,Cantor A,Kharbanda S,Johnson MR

    更新日期:2011-07-01 00:00:00

  • The murine common deletion: mitochondrial DNA 3,860-bp deletion after irradiation.

    abstract::This study demonstrates that mice, similar to humans, have a common mitochondrial DNA deletion (3,860 bp) that encodes 5 transfer RNA genes and 5 polypeptide genes that is related to aging, tissue type and radiotoxicity. Our research indicates that the deletion ratio in the liver was significantly higher than in the b...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR3373.1

    authors: Zhang SB,Maguire D,Zhang M,Zhang Z,Zhang A,Yin L,Zhang L,Huang L,Vidyasagar S,Swarts S,Okunieff P

    更新日期:2013-10-01 00:00:00

  • Radioprotection of mice with interleukin-1: relationship to the number of erythroid and granulocyte-macrophage colony-forming cells.

    abstract::This report presents the results of an investigation of changes in the number of erythroid and granulocyte-macrophage colony-forming cells (GM-CFC) that had occurred in tissues of normal B6D2F1 mice 20 h after administration of a radioprotective dose (150 ng) of human recombinant interleukin-1 (rIL-1). Neutrophilia in...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Schwartz GN,Patchen ML,Neta R,MacVittie TJ

    更新日期:1990-02-01 00:00:00

  • Interaction of lymphocytes and high endothelial venules in irradiated lymph nodes.

    abstract::After total-body exposure to various doses of ionizing radiation, the ability of lymphocytes to interact specifically with high endothelial venules of rat cervical and mesenteric lymph nodes was analyzed in frozen sections. Following a radiation dose of 1.5 Gy, high endothelial venules remained intact and the binding ...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Hale ML,Briggs R,McCarthy KF

    更新日期:1991-02-01 00:00:00

  • In situ detection of chromosome bridge formation and delayed reproductive death in normal human embryonic cells surviving X irradiation.

    abstract::We investigated delayed reproductive death and chromosome bridge formation in mitotic cells in colonies of normal human embryonic cells which survived exposure to 6 Gy of X rays. Eighteen primary clones each derived from control and irradiated cultures were isolated and secondary colonies were grown up. Six of the pri...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Suzuki K,Takahara R,Kodama S,Watanabe M

    更新日期:1998-10-01 00:00:00

  • Radiosensitivity of polyamine-depleted HeLa cells and modulation by the aminothiol WR-1065.

    abstract::The radiosensitivity of cultured HeLa cells was increased upon depletion of the natural cellular polyamines putrescine, spermidine and spermine through treatment of cultures with inhibitors of polyamine biosynthesis. This increased radiosensitivity was manifested as a decrease in the D0 and by the absence of a shoulde...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Snyder RD,Schroeder KK

    更新日期:1994-01-01 00:00:00

  • Further studies of the induction and intracellular repair of DNA strand breaks using intranuclear SV40 as a test system.

    abstract::We have previously published the techniques and preliminary results of an SV40 viral probe assay for gamma-radiation-induced single- and double-strand DNA breaks and their intracellular repair in higher cells (Radiat. Res. 101, 356-372, 1985). Those experiments with SV40 infected CV-1 monkey kidney cells suggested tha...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Krisch RE,Flick MB

    更新日期:1988-12-01 00:00:00

  • Decorporation of Pu/Am Actinides by Chelation Therapy: New Arguments in Favor of an Intracellular Component of DTPA Action.

    abstract::Diethylenetriaminepentaacetic acid (DTPA) is currently still the only known chelating drug that can be used for decorporation of internalized plutonium (Pu) and americium (Am). It is generally assumed that chelation occurs only in biological fluids, thus preventing Pu/Am deposition in target tissues. We postulate that...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:10.1667/RR14193.1

    authors: Grémy O,Laurent D,Coudert S,Griffiths NM,Miccoli L

    更新日期:2016-06-01 00:00:00

  • Hyperthermic sensitivity and growth stage in Escherichia coli.

    abstract::Hyperthermic sensitivities of Escherichia coli B/r and Bs-1 were determined for lag-, midlog-, and stationary-phase cells at 47, 48, and 49 degrees C. In both strains midlog-phase cells were strikingly more heat sensitive (100-fold greater killing after 4 h at 48 degrees C) than stationary-phase cells, with intermedia...

    journal_title:Radiation research

    pub_type: 杂志文章

    doi:

    authors: Yatvin MB,Gipp JJ,Klessig DR,Dennis WH

    更新日期:1986-04-01 00:00:00