Abstract:
:Vascular injury after radiation exposure contributes to multiple types of tissue injury through a cascade of events. Some of the earliest consequences of radiation damage include increased vascular permeability and promotion of inflammation, which is partially manifested by increased leukocyte-endothelial (L/E) interactions. We describe herein a novel intravital imaging method to evaluate L/E interactions, as a function of shear stress, and vascular permeability at multiple time points after local irradiation to the ear. This model permitted analysis of quiescent vasculature that was not perturbed by any surgical manipulation prior to imaging. To evaluate the effects of radiation on vascular integrity, fluorescent dextran was injected intravenously and its extravasation in the extravascular space surrounding the ear vasculature was measured at days 3 and 7 after 6 Gy irradiation. The vascular permeability rate increased approximately twofold at both days 3 and 7 postirradiation ( P < 0.05). Leukocyte rolling, which is indicative of L/E interactions, was significantly increased in mice at 24 h postirradiation compared to that of nonirradiated mice. To assess our model, as a means for assessing vascular radioprotectants, we treated additional cohorts of mice with a thrombopoietin mimetic, TPOm (RWJ-800088). In addition to stimulating platelet formation, thrombopoietin can protect vasculature after several forms of injury. Thus, we hypothesized that TPOm would reduce vascular permeability and L/E adhesion after localized irradiation to the ear vasculature of mice. If TPOm reduced these consequences of radiation, it would validate the utility of our intravital imaging method. TPOm reduced radiation-induced vascular leakage to control levels at day 7. Furthermore, L/E cell interactions were also reduced in irradiated mice treated with TPOm, compared with mice receiving irradiation alone, particularly at high shear stress ( P = 0.03, Kruskal-Wallis). We conclude that the ear model is useful for monitoring quiescent normal tissue vascular injury after radiation exposure. Furthermore, the application of TPOm, for preventing early inflammatory response created by damage to vascular endothelium, suggests that this drug may prove useful in reducing toxicities from radiotherapy, which damage microvasculature that critically important to tissue function.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Ashcraft KA,Choudhury KR,Birer SR,Hendargo HC,Patel P,Eichenbaum G,Dewhirst MWdoi
10.1667/RR14896.1subject
Has Abstractpub_date
2018-07-01 00:00:00pages
12-21issue
1eissn
0033-7587issn
1938-5404journal_volume
190pub_type
杂志文章abstract::Ion microscopy was used for subcellular quantitative imaging of the isotopes 10B and 11B in the same cell to evaluate boron delivery using a mixture of two neutron capture therapy drugs, p-boronophenylalanine-fructose (BPA-F) and sodium borocaptate (BSH). The application of 10B-labeled BPA-F and 11B-labeled BSH allowe...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)157[0700:qssims]2.0.co;2
更新日期:2002-06-01 00:00:00
abstract::The relationship between binucleate cell formation and the loss of colony-forming ability was examined in several cultured mammalian cell lines irradiated with X rays. The maximum fraction of binucleate cells after X irradiation increased dose-dependently within the range in which reproductive cell death might predomi...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::To date, there are no safe and effective drugs available for protection against ionizing radiation damage. Therefore, a great need exists to identify and develop non-toxic agents that will be useful as radioprotectors or postirradiation therapies under a variety of operational scenarios. We have developed a new pharma...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr2657.1
更新日期:2012-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-04-01 00:00:00
abstract::We examined the effects of X irradiation on activation of the promoter of the human HSP70B gene, which is activated only after heat shock. The HSP70B promoter was ligated to the bacterial beta-galactosidase (beta-gal) gene, and the reporter plasmid was transfected into NCI-H1299 carcinoma cells. The expression of the ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::The effects of ionizing radiation, with or without the anti-angiogenic agent anginex (Ax), on multiple myeloma growth were tested in a SCID-rab mouse model. Mice carrying human multiple myeloma cell-containing pre-implanted bone grafts were treated weekly with various regimens for 8 weeks. Rapid multiple myeloma growt...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1734.1
更新日期:2010-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::The effect of age at exposure on induction of mammary carcinoma was studied in female rats of the inbred WAG/Rij strain that were treated with estrogen. Groups of 40 animals were exposed to a single total-body dose of 1 or 2 Gy of 137Cs gamma radiation at age 8, 10, 12, 15, 22, 36 or 64 weeks. Hormone levels in the an...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::The study design and obtained results represent an intercomparison of various laboratories performing dose assessment using the dicentric chromosome analysis (DCA) as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibrat...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR3235.1
更新日期:2013-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)155[0785:odfrti]2.0.co;2
更新日期:2001-06-01 00:00:00
abstract::Wen, B., Urano, M., Humm, J. L., Seshan, V. E., Li, G. C. and Ling, C. C. Comparison of Helzel and OxyLite Systems in the Measurements of Tumor Partial Oxygen Pressure (pO(2)). Radiat. Res. 168, 67-75 (2008). It has been demonstrated in both experimental and human malignancies that hypoxic tumor cells are linked with ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0888.1
更新日期:2008-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR13862.1
更新日期:2015-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)157[0469:cmfbdb]2.0.co;2
更新日期:2002-04-01 00:00:00
abstract::A method was used to detect chromosome inversions as apparent or false sister chromatid exchanges (SCEs) in the first mitosis after gamma irradiation of human G0 cells. Dose-response relationships for small inversions have not been measured and reported previously, but it has been assumed that these are induced with a...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::The human breast is sensitive to radiation carcinogenesis, and genomic instability occurs early in breast cancer development. This study tests the hypothesis that ionizing radiation elicits genomic instability in finite life-span human mammary epithelial cells (HMEC) and asks whether densely ionizing radiation is a mo...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1317.1
更新日期:2008-07-01 00:00:00
abstract::Based on murine survival studies, endogenous hemopoietic spleen colony formation (E-CFU), and recovery of bone marrow and splenic granulocyte-macrophage colony-forming cells (GM-CFC), it was demonstrated that the postirradiation administration of glucan, an immunomodulator and hemopoietic stimulant, enhances the radio...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Nuclear protein and DNA content of HeLa cells was determined as a function of time following hyperthermia by staining isolated nuclei with two fluorescent dyes: fluorescein isothiocyanate (FITC) for protein content and propidium iodide (PI) for DNA content. Bivariate FITC and PI histograms were obtained by flow cytome...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::Infusions of boronophenylalanine-fructose complex (BPA-F), at doses up to 900 mg/kg of BPA and 860 mg/kg of fructose, have been used to deliver boron to cancer tissue for boron neutron capture therapy (BNCT). In patients with phenylketonuria (PKU), phenylalanine accumulates, which is harmful in the long run. PKU has b...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3067
更新日期:2003-11-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2419.1
更新日期:2011-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR3373.1
更新日期:2013-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::After total-body exposure to various doses of ionizing radiation, the ability of lymphocytes to interact specifically with high endothelial venules of rat cervical and mesenteric lymph nodes was analyzed in frozen sections. Following a radiation dose of 1.5 Gy, high endothelial venules remained intact and the binding ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::We investigated delayed reproductive death and chromosome bridge formation in mitotic cells in colonies of normal human embryonic cells which survived exposure to 6 Gy of X rays. Eighteen primary clones each derived from control and irradiated cultures were isolated and secondary colonies were grown up. Six of the pri...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::The radiosensitivity of cultured HeLa cells was increased upon depletion of the natural cellular polyamines putrescine, spermidine and spermine through treatment of cultures with inhibitors of polyamine biosynthesis. This increased radiosensitivity was manifested as a decrease in the D0 and by the absence of a shoulde...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::We have previously published the techniques and preliminary results of an SV40 viral probe assay for gamma-radiation-induced single- and double-strand DNA breaks and their intracellular repair in higher cells (Radiat. Res. 101, 356-372, 1985). Those experiments with SV40 infected CV-1 monkey kidney cells suggested tha...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::Diethylenetriaminepentaacetic acid (DTPA) is currently still the only known chelating drug that can be used for decorporation of internalized plutonium (Pu) and americium (Am). It is generally assumed that chelation occurs only in biological fluids, thus preventing Pu/Am deposition in target tissues. We postulate that...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14193.1
更新日期:2016-06-01 00:00:00
abstract::Hyperthermic sensitivities of Escherichia coli B/r and Bs-1 were determined for lag-, midlog-, and stationary-phase cells at 47, 48, and 49 degrees C. In both strains midlog-phase cells were strikingly more heat sensitive (100-fold greater killing after 4 h at 48 degrees C) than stationary-phase cells, with intermedia...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00