Abstract：
:Exposure of cells to heat induces thermotolerance, a transient resistance to subsequent heat challenges. It has been shown that thermotolerance is correlated in time with the enhanced synthesis of heat shock proteins. In this study, the association of induced heat shock proteins with various cellular fractions was investigated and the heat-induced changes in skeletal protein composition in thermotolerant and control cells was compared. All three major heat shock proteins induced in Chinese hamster fibroblasts after a 46 degrees C, 4-min heat treatment (70, 87, and 110 kDa) were purified with the cytoplasmic fraction, whereas only the 70-kDa protein was also found in other cell fractions, including that containing the cellular skeleton. Immediately after a second heat treatment at 45 degrees C for 45 min, the 110-kDa protein from thermotolerant cells also purified extensively with the cellular skeletal fraction. In this regard, the 110-kDa protein behaved similarly to many other cellular proteins, since we observed an overall temperature-dependent increase in the total labeled protein content of the high-salt-resistant cellular skeletal fraction after heat shock. Pulse-chase studies demonstrated that this increased protein content gradually returned to normal levels after a 3-hr incubation at 37 degrees C. The alteration or recovery kinetics of the total labeled protein content of the cellular skeletal fraction after heat shock did not correlate with the dramatic increase in survival observed in thermotolerant cells. The relationship between heat shock proteins and thermotolerance, therefore, does not correlate directly with changes in the heat-induced cellular alterations leading to differences in protein fractionation.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Murnane JP,Li GCsubject
Has Abstractpub_date
1985-03-01 00:00:00pages
480-90issue
3eissn
0033-7587issn
1938-5404journal_volume
101pub_type
杂志文章abstract：:Vascular change by the combined treatments of radiation and hyperthermia was studied. The legs of rats were irradiated with 2000 rad of X rays in a single dose and heated at 43.0 degrees C for 1 hr at various times after irradiation, and the blood flow and vascular volume in the skin and muscle were determined with 12...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Human erythrocytes were isolated from the blood of healthy donors and then incubated in the presence of suspensions of alpha-tocopherol for 30 min at 37 degrees C. Unabsorbed tocopherol was removed by centrifugation using several washes of isotonic phosphate-buffered saline. Washed erythrocytes were resuspended to 0.0...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：:The capacity of plateau-phase Chinese hamster V79 and normal and transformed C3H-10T1/2 cells for repair of potentially lethal radiation damage (PLD) was evaluated for cells irradiated alone or given combined treatments of heat and radiation. The data show that all cell lines tested could repair PLD and that transform...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:The Radiological Research Accelerator Facility (RARAF) is in its 50th year of operation. It was commissioned on April 1, 1967 as a collaboration between the Radiological Research Laboratory (RRL) of Columbia University, and members of the Medical Research Center of Brookhaven National Laboratory (BNL). It was initiall...
journal_title：Radiation research
pub_type： 历史文章,杂志文章
doi：10.1667/RR002CC.1
更新日期：2017-04-01 00:00:00
abstract：:This study investigated whether the clonogenic ability of tumor cells to establish subcutaneous tumors and the tumor-associated macrophage (TAM) content correlate with the development and extent of the tumor bed effect (TBE). Ten tumors, five sarcomas and five carcinomas, syngeneic to C3Hf/Kam mice were used. Tumors w...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:The effect of acute exposure to ultrawideband (UWB) electromagnetic radiation on the Primate Equilibrium Platform (PEP) task, where the monkey's task is to manipulate a joystick control to compensate for the random perturbations in the pitch plane that are generated by a computer at unpredictable intervals, was examin...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:A new model for the survival of bacteria exposed to ionizing radiation is constructed in the framework of a target theory based on microdosimetric concepts, where single- and double-strand breaks of DNA and their repair in vivo can be described consistently in terms of the microdosimetric quantity j (number of effecti...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Single-strand breaks (SSBs) and double-strand breaks (DSBs) induced in DNA under phosphorus K-shell resonant absorption have been studied using supercoiled plasmids. The kinetics of the production of SSBs and DSBs exhibits a linear and a quadratic dependence, respectively, on photon fluence. Cross sections and quantum...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:To ensure the high-fidelity transmission by reproductive cells of genetic information from generation to generation, cells have evolved surveillance systems to eliminate genomic lesions by inducing cell suicide and/or DNA repair. In this report, gamma-ray-induced cell death was investigated using the medaka fish, Oryz...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0386:acsisf]2.0.co;2
更新日期：2002-04-01 00:00:00
abstract：:The flow cytometry mutation assay is based on detecting mutations in the CD59 gene on human chromosome 11 in CHO A(L) cells with flow cytometry, but the kinetics of mutant expression and the histogram region for mutant selection have not been studied in detail. CHO A(L) cells were analyzed by flow cytometry for CD59 e...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1276.1
更新日期：2008-11-01 00:00:00
abstract：:We examined the effects of X irradiation on activation of the promoter of the human HSP70B gene, which is activated only after heat shock. The HSP70B promoter was ligated to the bacterial beta-galactosidase (beta-gal) gene, and the reporter plasmid was transfected into NCI-H1299 carcinoma cells. The expression of the ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:Changes in the free-cell population of the lungs of two strains of mice (SAS/4 and CBA/H) were studied up to 4 months after inhalation exposure to a sized fraction of 239PuO2 particles (1.5 micron AMAD) to give initial alveolar depositions (IADs) ranging from 17 to 810 Bq. A sample of the free-cell population of the l...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:New data are given on the use of oxalic acid, in light and heavy water solutions, for in-pile chemical dosimetry. The method of calculating the absorbed doses in low-Z materials is described. Results of chemical measurements in the core of the heavy-water-moderated reactor at Vinca (Yugoslavia) are compared with the r...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-12-01 00:00:00
abstract：:A biphasic curve was observed when surviving allogeneic lytic activity was plotted as a function of irradiation delivered before sensitization. Flow cytometry analysis demonstrated that the number of cells was reduced in response to increasing dose and that subset precursors Lyt 1+2+ were proportionally more radiosens...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:Following X irradiation of 3',5'-cyclic guanosine monophosphate single crystals at 10 K, several free radicals were trapped and detected by ESR/ENDOR/FSE spectroscopy. The two dominant species both have unpaired spin located on the guanine base. One is the product of net hydrogen atom loss from the exocyclic amino gro...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:After total-body exposure to various doses of ionizing radiation, the ability of lymphocytes to interact specifically with high endothelial venules of rat cervical and mesenteric lymph nodes was analyzed in frozen sections. Following a radiation dose of 1.5 Gy, high endothelial venules remained intact and the binding ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:The mortality experience between 1956 and 1985 of 8977 males employed by Atomic Energy of Canada Limited is reported. A total of 4260 men, 47% of the cohort, were exposed to low doses of external ionizing radiation at low dose rates, with a mean cumulative equivalent dose of 52.1 mSv. For cancers as a whole the excess...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:The microdosimetry of (10)B thermal neutron capture reactions should be considered as an essential step to be followed before studying the radiobiological aspects of boron neutron capture therapy. The boron dose itself is insufficient as the only quantity used to describe the biological effectiveness of the (10)B reac...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3257
更新日期：2004-12-01 00:00:00
abstract：:The incidence of micronuclei was evaluated to assess the induction of an adaptive response to non-ionizing radiofrequency (RF) radiation in peripheral blood lymphocytes collected from five different human volunteers. After stimulation with phytohemagglutinin for 24 h, the cells were exposed to an adaptive dose of 900 ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1687.1
更新日期：2009-06-01 00:00:00
abstract：:Hyperthermia sensitization or tolerance is subject to cellular events that may occur at membrane, nuclear, and cytoplasmic sites. We have studied the effects of elevated temperatures on the oxidative-reductive state of the cell by measuring and altering glutathione (GSH) concentrations. GSH plays a pivotal role in mai...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-09-01 00:00:00
abstract：:Single crystals of guanine hydrochloride monohydrate, guanine hydrochloride dihydrate and anhydrous guanine dihydrochloride, doped with thioguanine, were irradiated with X and gamma rays. In all three systems the dominant radicals were associated with thioguanine. In the former two systems the stabilized species is th...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:Synthetic lysophospholipids represent a variety of analogs of the naturally occurring 2-lysophosphatidylcholine. Some of these compounds showed significant therapeutic effects on the survival of mice following radiation injury when administered after various doses of whole-body X irradiation. Such therapeutic effects ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-04-01 00:00:00
abstract：:The electroporation of restriction enzymes into mammalian cells results in DNA double-strand breaks that can lead to chromosome aberrations. Four chemicals known to interfere with cellular responses to DNA damage were investigated for their effects on chromosome aberrations induced by AluI and Sau3AI; in addition, the...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Experiments were performed with three different cell lines, mouse fibroblast LM cells, HeLa S3 cells, and Ehrlich Ascites Tumor (EAT) cells, to establish the possible importance of hyperthermic-induced alterations in cellular K+ content in the mechanism of cell killing by heat. At different time points after the hyper...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:Beagle dogs had signs of restrictive lung disease 1 to 5 years after exposure by inhalation to 239PuO2 aerosols. The 239PuO2 aerosols were monodisperse with activity median aerodynamic diameters of 0.75, 1.5, or 3.0 microns. The plutonium particles produced protracted alpha irradiation of the lungs. Ten dogs had speci...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:The modifying effects of m-aminobenzamide (m-ABA), an inhibitor of poly(ADP-ribose) synthesis, on 42 degrees C hyperthermia- and/or radiation-induced cell killing were examined in Chinese hamster V-79 cells. When cells were exposed to 42 degrees C hyperthermia in combination with m-ABA (10 mM), cell survival decreased...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:Solid thymidylyl-(3'-->5')-thymidine (dTpdT) was irradiated in a vacuum with monochromatic photons from 150 to 300 nm; the photoproducts were analyzed quantitatively by high-performance liquid chromatography. The results of the experiment were as follows: (1) above 210 nm the major photoproducts were three dithymine p...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:To determine whether exposure to radiofrequency (RF) radiation can induce DNA damage or apoptosis, Molt-4 T lymphoblastoid cells were exposed with RF fields at frequencies and modulations of the type used by wireless communication devices. Four types of frequency/modulation forms were studied: 847.74 MHz code-division...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3127
更新日期：2004-02-01 00:00:00
abstract：:Although skin is usually exposed during human exposures to ionizing radiation, there have been no thorough examinations of the transcriptional response of skin fibroblasts and keratinocytes to radiation. The transcriptional response of quiescent primary fibroblasts and keratinocytes exposed to from 10 cGy to 5 Gy and ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1677.1
更新日期：2009-07-01 00:00:00
abstract：:A standard Fricke dosimeter was used to measure the absorbed dose via the oxidation yields of Fe3+ ions in an aqueous environment induced by soft X rays within the "water window" spectral range. We also exploited the property of a neutral solution containing terephthalic acid as a tool for selective detection of OH ra...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15520.1
更新日期：2020-04-01 00:00:00
