Abstract：
:Although skin is usually exposed during human exposures to ionizing radiation, there have been no thorough examinations of the transcriptional response of skin fibroblasts and keratinocytes to radiation. The transcriptional response of quiescent primary fibroblasts and keratinocytes exposed to from 10 cGy to 5 Gy and collected 4 h after treatment was examined. RNA was isolated and examined by microarray analysis for changes in the levels of gene expression. Exposure to ionizing radiation altered the expression of 279 genes across both cell types. Changes in RNA expression could be arranged into three main categories: (1) changes in keratinocytes but not in fibroblasts, (2) changes in fibroblasts but not in keratinocytes, and (3) changes in both. All of these changes were primarily of p53 target genes. Similar radiation-induced changes were induced in immortalized fibroblasts or keratinocytes. In separate experiments, protein was collected and analyzed by Western blotting for expression of proteins observed in microarray experiments to be overexpressed at the mRNA level. Both Q-PCR and Western blot analysis experiments validated these transcription changes. Our results are consistent with changes in the expression of p53 target genes as indicating the magnitude of cell responses to ionizing radiation.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Warters RL,Packard AT,Kramer GF,Gaffney DK,Moos PJdoi
10.1667/RR1677.1subject
Has Abstractpub_date
2009-07-01 00:00:00pages
82-95issue
1eissn
0033-7587issn
1938-5404journal_volume
172pub_type
杂志文章abstract：:Involvement of gap junctional intercellular communication (GJIC) in bystander responses of confluent human fibroblasts irradiated with a carbon-ion beam was investigated. It was found that the lower the radiation dose, the higher the yield of radiation-induced micronuclei per nuclear traversal, suggesting the existenc...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3044
更新日期：2003-09-01 00:00:00
abstract：:The severe combined immune deficiency (SCID) mouse shows two kinds of phenotypic abnormalities, a high radiosensitivity and an abnormal immunoglobulin gene recombination. A genetic study has revealed that a mutation exists in chromosome 16. However, several attempts to isolate the gene responsible for these phenotypes...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:Dose-spread kernels can be used to calculate the dose distribution in a photon beam by convolving the kernel with the primary fluence distribution. The theoretical relationships between various types and components of dose-spread kernels relative to photon attenuation coefficients are explored. These relations can be ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:These studies examined the effects of X radiation and interleukin 3 (IL-3), which is an effective cytokine for the generation of megakaryocytopoiesis from X-irradiated hematopoietic stem/progenitor cells, on the terminal process of human megakaryocytopoiesis and thrombopoiesis. Mature megakaryocytes were induced by cu...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1519.1
更新日期：2009-09-01 00:00:00
abstract：:Numerous studies have shown that histone deacetylase inhibitors (HDACis) improve cellular acetylation while also enhancing the radiation sensitivity. In this work, however, we confirmed that low-dose trichostatin A (TSA) as a typical HDACi could reduce rather than increase the radiosensitivity of cancer cells, while t...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RADE-20-00145.1
更新日期：2021-01-29 00:00:00
abstract：:While impairment of thyroid function has been demonstrated for high-dose external radiation (e.g., for Hodgkin's disease), the long-term functional effects of low-dose external radiation have not been fully explored. One hundred fifty-three subjects with a past history of thymic irradiation during infancy were stratif...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:This continues the series of periodic general reports on cancer mortality in the cohort of A-bomb survivors followed by the Radiation Effects Research Foundation. The follow-up is extended by the 5 years 1986-1990, and analysis includes an additional 10,500 survivors with recently estimated radiation doses. Together t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Domestic radon risk estimates are typically based either on data for uranium miners or on data derived from A-bomb survivors; comparison of domestic radon risk estimates derived from these two disparate sources represents an important test of their reliability. There is currently a significant discrepancy of about a f...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:Chromatid breaks have previously been shown to be induced in G2-phase cells after exposure to ionizing radiation (X and gamma rays) as a linear function of dose, consistent with a single-event mechanism. DNA double-strand breaks (DSBs) are thought to be the initiating lesion, and experiments with a genetically enginee...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2003)159[0247:iocbbc]2.0.co;2
更新日期：2003-02-01 00:00:00
abstract：:The flow cytometry mutation assay is based on detecting mutations in the CD59 gene on human chromosome 11 in CHO A(L) cells with flow cytometry, but the kinetics of mutant expression and the histogram region for mutant selection have not been studied in detail. CHO A(L) cells were analyzed by flow cytometry for CD59 e...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1276.1
更新日期：2008-11-01 00:00:00
abstract：:The goal of this study was to make a comparison between stereotactic brachytherapy implants and linear accelerator-based radiosurgery of brain tumors with respect to physical dose distributions and radiobiological efficacy. Twenty-four treatment plans made for irradiation of brain tumors with low-dose-rate (125)I brac...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3529.1
更新日期：2006-06-01 00:00:00
abstract：:Based on encouraging results from several early-phase clinical trials, there is renewed interest in the use of pharmacological ascorbate (i.e., intravenous administration resulting in >≈10 mM plasma ascorbate concentrations) in combination with standard-of-care cancer treatments including radiation and/or chemotherapy...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15328.1
更新日期：2019-06-01 00:00:00
abstract：:To approach treatment of micrometastases of steroid receptor-rich cancers using estrogen receptor-directed therapy with Auger electrons, multicellular spheroids of the estrogen receptor-rich human breast cancer cell line, MCF-7, were prepared and exposed to a range of concentrations of an Auger electron-emitting estro...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:The monohydroxylated fatty acid content of peripheral blood mononuclear cells from 23 cleanup workers and 16 unexposed individuals was studied in relation to their immune status after the Chernobyl accident. Men with absorbed doses below 0.32 Gy showed higher levels of free and esterified 12-hydroxyeicosatetraenoic ac...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)156[0476:mfacip]2.0.co;2
更新日期：2001-11-01 00:00:00
abstract：:A biphasic curve was observed when surviving allogeneic lytic activity was plotted as a function of irradiation delivered before sensitization. Flow cytometry analysis demonstrated that the number of cells was reduced in response to increasing dose and that subset precursors Lyt 1+2+ were proportionally more radiosens...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:Isogenic Escherichia coli strains carrying single DNA-repair mutations were compared for their capacity for (i) the repair of X-ray-induced DNA double-strand breaks (DSB) as measured using neutral sucrose gradients; (ii) medium-dependent resistance, i.e., a recA-dependent X-ray survival phenomenon that correlates clos...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:Molecular oxygen has long been recognized as a powerful radiosensitizer that enhances the cell-killing efficiency of ionizing radiation. Radiosensitization by oxygen occurs at very low concentrations with the half-maximum radiosensitization at approximately 3 mmHg. However, robust hypoxia-induced signal transduction c...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/RR13959.1
更新日期：2015-05-01 00:00:00
abstract：:A formalism developed in the first paper in this series, based on the generalized Theory of Dual Radiation Action, is applied to survival data for cells exposed to soft X rays. It is shown that, in contrast to previous published analyses, this formalism--modified to account for the attenuation of dose across the sensi...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:The investigation of fragment length distributions of plasmid DNA gives insight into the influence of localized energy distribution on the induction of DNA damage, particularly the clustering of double-strand breaks. We present an approach that determines the fragment length distributions of plasmid DNA after heavy-io...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1028.1
更新日期：2008-06-01 00:00:00
abstract：:The functional and morphological response of the remaining hypertrophied kidney in unilaterally nephrectomized rats to single doses of 0-20 Gy X rays was investigated. Functional and histological end points were assessed serially 4-24 weeks postirradiation. Renal irradiation led to time- and dose-dependent reductions ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0481:trotti]2.0.co;2
更新日期：2001-03-01 00:00:00
abstract：:Plateau-phase A549 cells exhibit a high capacity for repair of potentially lethal radiation damage (PLD). Previously it was found that PLD repair could be partially inhibited by increasing the extracellular pH (pHe) of the spent medium from its normal value of 6.7-6.8 to 7.6 during postirradiation holding. The present...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:Dose-response relationships for X-ray-induced reciprocal translocations in spermatogonial stem cells of mutant and wild-type mice were established by spermatocyte analysis many cell generations after irradiation. The mutants studied were Wv/+, the viable allele of dominant spotting in the heterozygous state, and Slcon...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:This study was undertaken to determine if structural alterations of the bulk chromatin and the amount of protein associated with the nuclear matrix in cerebellar neurons depend on radiation dose and a cell's state of oxygenation. After irradiation with 2.5 to 25.0 Gy under both aerobic and anoxic conditions, the sensi...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:Radiation-induced heart disease (RIHD) is a serious side effect of radiotherapy for intrathoracic and chest wall tumors. The threshold dose for development of clinically significant RIHD is believed to be lower than previously assumed. Therefore, research into mechanisms of RIHD has gained substantial momentum. RIHD b...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/rr2933.1
更新日期：2012-07-01 00:00:00
abstract：:The mortality experience between 1956 and 1985 of 8977 males employed by Atomic Energy of Canada Limited is reported. A total of 4260 men, 47% of the cohort, were exposed to low doses of external ionizing radiation at low dose rates, with a mean cumulative equivalent dose of 52.1 mSv. For cancers as a whole the excess...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Pulmonary carcinomas were recorded in a life-span experiment of male Sprague-Dawley rats exposed to fission neutrons. Mortality-corrected prevalences are obtained by the method of isotonic regression. In a second part of the paper a comparison is made with data obtained earlier for radon-daughter inhalations in the sa...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：:Clinicians involved in conventional radiation therapy are very concerned about the dose-response relationships of normal tissues. Before proceeding to new clinical protocols, radiation biologists involved with conformal proton therapy believe it is necessary to quantify the dose response and tolerance of the organs an...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/r3016
更新日期：2003-07-01 00:00:00
abstract：:This review was initiated in response to concerns that ionizing radiation could be a cause of melanoma. Studies presenting the relative risks for melanoma after external ionizing radiation exposure were in seven categories: (1) The Canadian Radiation Dose Registry, (2) nuclear industry workers, (3) subjects near nucle...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/rr3447.1
更新日期：2005-11-01 00:00:00
abstract：:Tissue oxygenation influences the radiation response of tumors. To further investigate the underlying mechanisms of tumor hypoxia, the spatial distribution of hypoxic cells in relation to the vasculature was studied. In a panel of three human glioma xenograft lines (E2, E102, E106) with different growth characteristic...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0626:qaovpo]2.0.co;2
更新日期：2002-06-01 00:00:00
abstract：:The well-established process of potentially lethal damage (PLD) repair enhances plateau-phase cell survival after exposure to ionizing radiation. PLD repair requires that confluent cells be incubated prior to plating for a colony-forming assay rather than being plated immediately. Enhanced double-strand break (DSB) re...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr1423.1
更新日期：2008-10-01 00:00:00
