Abstract：
:The non-random distribution of DNA breakage in PFGE (pulsed-field gel electrophoresis) experiments poses a problem of proper subtraction of the background DNA damage to obtain a fragment-size distribution due to radiation only. A naive bin-to-bin subtraction of the background signal will not result in the right DNA mass distribution histogram. This problem could become more pronounced for high-LET (linear energy transfer) radiation, because the fragment-size distribution manifests a higher frequency of smaller fragments. Previous systematic subtraction methods have been based on random breakage, appropriate for low-LET radiation. Moreover, an investigation is needed to determine whether the background breakage is itself random or non-random. We consider two limiting cases: (1) the background damage is present in all cells, and (2) it is present in only a small subset of cells, while other cells are not contributing to the background DNA fragmentation. We give a generalized formalism based on stochastic processes for the subtraction of the background damage in PFGE experiments for any LET and apply it to two sets of PFGE data for iron ions.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Ponomarev AL,Belli M,Hahnfeldt PJ,Hlatky L,Sachs RK,Cucinotta FAdoi
10.1667/RR0663.1subject
Has Abstractpub_date
2006-12-01 00:00:00pages
908-16issue
6eissn
0033-7587issn
1938-5404pii
RR0663journal_volume
166pub_type
杂志文章abstract：:Reverse-biased silicon p-n junction arrays using Silicon-On-Insulator technology have been proposed as microdosimeters. The performance of such detectors in boron neutron capture therapy (BNCT) is discussed. This work provides the first reported measurements using boron-coated silicon diode arrays as microdosimeters i...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:The objective of this study was to determine whether a deficiency for either one of two repair processes influences the phenomenon of enhancement of radiation-induced cell killing by carboplatin which has been reported previously in one cell line (V79) and which is presumably a result of an interaction between these t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Water:n-octanol partition coefficients (KD) were determined for a series of radioprotective thiols to ascertain whether these could be used to estimate reliably their rates of uptake into mammalian cells by passive diffusion. Values of KD determined for thiols in 0.1 M potassium phosphate, pH 7.4, at 22 degrees C were...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Damage to intestine is a serious problem after accidental radiation exposure. To examine substances to ameliorate damage by postirradiation administration, we focused on the regeneration process after irradiation of the intestine. Using experimental systems, the effects of clinically used sex hormones on regeneration ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2154.1
更新日期：2011-03-01 00:00:00
abstract：:This study investigated the biological effects and adaptive responses induced by single and repeated in vivo computed tomography (CT) scans. We postulated that, through the induction of low-level oxidative stress, repeated low-dose CT scans (20 mGy, 2 days/week, 10 weeks) could protect mice (C57BL/6) from acute effect...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2532.1
更新日期：2012-02-01 00:00:00
abstract：:Studies have causally linked external thyroid radiation exposure in childhood with thyroid cancer. In 1995, investigators conducted relative risk analyses of pooled data from seven epidemiologic studies. Doses were mostly <10 Gy, although childhood cancer therapies can result in thyroid doses >50 Gy. We pooled data fr...
journal_title：Radiation research
pub_type： 杂志文章,meta分析
doi：10.1667/RR14213.1
更新日期：2016-05-01 00:00:00
abstract：:Radiation-induced free radical damage in solid-state DNA arises from direct-type damage mechanisms. In the present study, free radical yields in film and lyophilized Na DNA model systems are compared. The free radical yields in lyophilized samples are significantly greater than those in films. Since DNA conformation c...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:In a scenario involving a nuclear detonation during war or a terrorist attack, acute radiation exposure combined with thermal and blast effects results in severe skin injury. Although the cutaneous injury in such a scenario may not be lethal, it may lead to inflammation, delayed wound healing and loss of the skin barr...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14884.1
更新日期：2018-04-01 00:00:00
abstract：:There has been ongoing debate and discussion concerning whether the funding source influenced the outcome of research on human health effects from exposure to radiofrequency fields (RF, electromagnetic waves that carry energy as they propagate in air and dense media). In a study of 225 publications, in which we sought...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15364.1
更新日期：2019-10-01 00:00:00
abstract：:The repair of double-strand breaks in mammalian cells is carried out by two pathways: homologous recombination and nonhomologous end joining. The factors that regulate the mechanism through which a specific repair pathway is activated are still not clearly defined. To study whether the complexity of the double-strand ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1487.1
更新日期：2009-04-01 00:00:00
abstract：:Given the distinct characteristics of low-energy electrons (LEEs), particularly at energies less than 30 eV, they can be applied to a wide range of therapeutic modalities to improve cancer treatment. LEEs have been shown to efficiently produce complex molecular damage resulting in substantial cellular toxicities. Sinc...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/RR14727.1
更新日期：2017-08-01 00:00:00
abstract：:In previous studies we determined a gene expression signature in baboons for predicting the severity of hematological acute radiation syndrome. We subsequently validated a set of eight of these genes in leukemia patients undergoing total-body irradiation. In the current study, we addressed the effect of intra-individu...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15013.1
更新日期：2018-09-01 00:00:00
abstract：:Evidence is presented for the formation of products in irradiated dinucleoside monophosphates in which both bases are damaged. The dinucleoside monophosphates d(GpT), d(GpC), d(TpG) and d(CpG) were X-irradiated in oxygenated aqueous solution. Product identification was by NMR spectroscopy. In products containing doubl...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:The modifying effects of m-aminobenzamide (m-ABA), an inhibitor of poly(ADP-ribose) synthesis, on 42 degrees C hyperthermia- and/or radiation-induced cell killing were examined in Chinese hamster V-79 cells. When cells were exposed to 42 degrees C hyperthermia in combination with m-ABA (10 mM), cell survival decreased...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:The relationships between DNA damage and the survival of murine bone marrow cells irradiated in situ were examined. Cell survival was assayed by the ability of bone marrow cells from irradiated mice to form colonies in vitro (CFU-C). DNA double-strand breaks (DSBs) were measured by neutral (nondenaturing) filter eluti...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:The structural maintenance of chromosome 1 (Smc1) protein is a member of the highly conserved cohesin complex and is involved in sister chromatid cohesion. In response to ionizing radiation, Smc1 is phosphorylated at two sites, Ser-957 and Ser-966, and these phosphorylation events are dependent on the ATM protein kina...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2402.1
更新日期：2011-03-01 00:00:00
abstract：:The dependence of relative biological effectiveness (RBE) on photon energy is a topic of extensive discussions. The increasing amount of in vitro data in the low-energy region indicates this to be a complex dependence that is influenced by the end point and cell line studied. In the present investigation, the RBE of 1...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0874.1
更新日期：2008-03-01 00:00:00
abstract：:There are public concerns regarding possible carcinogenic or cancer-promoting effects of electromagnetic fields (EMFs) from mobile phones and base stations. The objective of the present study was to investigate whether chronic exposure to EMFs of the UMTS (Universal Mobile Telecommunication System) influences the deve...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0857.1
更新日期：2007-07-01 00:00:00
abstract：:The LD50 of 46 salts of metals and rare earths (lanthanoids) was determined in mice. Half the LD50 of the compounds was then combined with lethal radiation (10.5 Gy) and the modification of survival time was scored. Only the metals beryllium and indium and the rare earths cerium, lanthanum and scandium displayed activ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:After a planned or unplanned radiation exposure, determination of absorbed dose has great clinical importance, informing treatment and triage decisions in the exposed individuals. Biodosimetry approaches allow for determination of dose in the absence of physical measurement apparatus. The current state-of-the-art biod...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15283.1
更新日期：2019-05-01 00:00:00
abstract：:The success of boron neutron capture therapy (BNCT) mainly depends on the boron concentration in the tumor and a high tumor/normal tissue (T/N) boron ratio or a high tumor/blood (T/B) boron ratio. Therefore, the effective enhancement of boron ratios is the first priority. Our study investigated whether a low-dose of γ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RADE-20-00102.1
更新日期：2021-01-29 00:00:00
abstract：:To determine whether different agents that enhance the expression of potentially lethal X-ray damage (PLD) interact with the same or different lesions (or spectrum of lesions), cell killing was measured in three kinds of experiments: (1) When cells were irradiated in G1 phase and treated with caffeine or hydroxyurea a...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:Radioprotection in vitro by sulfhydryl (SH)-containing compounds is usually greater in aerated than in hypoxic cells. This observation has been cited recently as one of the reasons for the relatively greater effectiveness of radioprotectors such as WR-2721 in normal tissues compared to tumor cells. It is demonstrated ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:Several studies over recent years have highlighted the possibility that radiation can induce transmissible genomic instability. Most of these involve in vitro irradiation and usually in vitro culture. Here it is reported that the short-half-life bone-seeking alpha-particle emitter (224)Ra did not induce excess transmi...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0345:lodtci]2.0.co;2
更新日期：2001-02-01 00:00:00
abstract：:The primary risk factors of multiple myeloma are age, race and sex, but several studies have found an association between radiological hazards and multiple myeloma. The purpose of this nested case-control study was to investigate whether workers with chronic low-level exposure to internally deposited uranium at the Oa...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1607.1
更新日期：2009-06-01 00:00:00
abstract：:Ionizing radiations induce mutations which can be detected both in coding sequences (Hprt locus) by measuring the frequency of 6-thioguanine-resistant cells and in minisatellite sequences by DNA fingerprint analysis. We analyzed the effects of irradiation with low-energy protons (31 keV/pm) and, for comparison, with u...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:To compare the genotoxic effects of high-LET ionizing radiation to those of low-LET radiation, we investigated the responses of human lymphoblastoid cells to DNA damage TK6 after treatment with either low-LET X rays or high-LET iron ions (1000 keV/microm). A highly localized distribution of gammaH2AX/RAD51 foci was ob...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0678:dccpih]2.0.co;2
更新日期：2002-12-01 00:00:00
abstract：:Radiotherapy is one of the major treatment modalities for the management of various cancers, however, it is limited by the severe side effects and complications experienced by some patients. Nicaraven, a chemically synthesized hydroxyl radical-specific scavenger, has been shown to protect normal tissues from radiation...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14614.1
更新日期：2017-02-22 00:00:00
abstract：:We have developed a new detection method for measuring distributions of energy depositions from particles characterized by low linear energy transfers (LETs). In particular, we have developed a detection system to measure energy depositions produced by electrons and photons on nanometer scales. The detector is based u...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Current and potential shortfalls in the number of radiation scientists stand in sharp contrast to the emerging scientific opportunities and the need for new knowledge to address issues of cancer survivorship and radiological and nuclear terrorism. In response to these challenges, workshops organized by the Radiation R...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3096
更新日期：2003-12-01 00:00:00
