Primary DNA damage in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation.


:Human peripheral blood samples collected from three healthy human volunteers were exposed in vitro to pulsed-wave 2450 MHz radiofrequency (RF) radiation for 2 h. The RF radiation was generated with a net forward power of 21 W and transmitted from a standard gain rectangular antenna horn in a vertically downward direction. The average power density at the position of the cells in the flask was 5 mW/cm(2). The mean specific absorption rate, calculated by finite difference time domain analysis, was 2.135 (+/-0.005 SE) W/kg. Aliquots of whole blood that were sham-exposed or exposed in vitro to 50 cGy of ionizing radiation from a (137)Cs gamma-ray source were used as controls. The lymphocytes were examined to determine the extent of primary DNA damage (single-strand breaks and alkali-labile lesions) using the alkaline comet assay with three different slide-processing schedules. The assay was performed on the cells immediately after the exposures and at 4 h after incubation of the exposed blood at 37 +/- 1 degrees C to allow time for rejoining of any strand breaks present immediately after exposure, i.e. to assess the capacity of the lymphocytes to repair this type of DNA damage. At either time, the data indicated no significant differences between RF-radiation- and sham-exposed lymphocytes with respect to the comet tail length, fluorescence intensity of the migrated DNA in the tail, and tail moment. The conclusions were similar for each of the three different comet assay slide-processing schedules examined. In contrast, the response of lymphocytes exposed to ionizing radiation was significantly different from RF-radiation- and sham-exposed cells. Thus, under the experimental conditions tested, there is no evidence for induction of DNA single-strand breaks and alkali-labile lesions in human blood lymphocytes exposed in vitro to pulsed-wave 2450 MHz radiofrequency radiation, either immediately or at 4 h after exposure.


Radiat Res


Radiation research


Vijayalaxmi,Leal BZ,Szilagyi M,Prihoda TJ,Meltz ML




Has Abstract


2000-04-01 00:00:00












  • The influence of packing on free radical yields in solid-state DNA: film compared to lyophilized frozen solution.

    abstract::Radiation-induced free radical damage in solid-state DNA arises from direct-type damage mechanisms. In the present study, free radical yields in film and lyophilized Na DNA model systems are compared. The free radical yields in lyophilized samples are significantly greater than those in films. Since DNA conformation c...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Milano MT,Bernhard WA

    更新日期:1999-08-01 00:00:00

  • Nicaraven, a Potential Radioprotective Agent, has Very Limited Effects on the Survival of Cancer Cells and the Growth of Established Tumors.

    abstract::Radiotherapy is one of the major treatment modalities for the management of various cancers, however, it is limited by the severe side effects and complications experienced by some patients. Nicaraven, a chemically synthesized hydroxyl radical-specific scavenger, has been shown to protect normal tissues from radiation...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Yan C,Luo L,Urata Y,Goto S,Guo CY,Li TS

    更新日期:2017-02-22 00:00:00

  • Prolongation of life span in the accelerated aging klotho mouse model, by low-dose-rate continuous γ irradiation.

    abstract::While lifespan studies provide basic information for estimating the risk of ionizing radiation, findings on the effect of low-dose/low-dose-rate irradiation on the lifespan of mammals are controversial. Here we evaluate the effect of continuous exposure to low-dose-rate γ radiation on the lifespan of mice with acceler...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Nomura T,Sakai K,Ogata H,Magae J

    更新日期:2013-06-01 00:00:00

  • Up-regulation and activation of proteinase-activated receptor 2 in early and delayed radiation injury in the rat intestine: influence of biological activators of proteinase-activated receptor 2.

    abstract::Proteinase-activated receptor 2 (Par2, F2rl1, also designated PAR-2 or PAR2) is prominently expressed in the intestine and has been suggested as a mediator of inflammatory, mitogenic and fibrogenic responses to injury. Mast cell proteinases and pancreatic trypsin, both of which have been shown to affect the intestinal...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Wang J,Zheng H,Hollenberg MD,Wijesuriya SJ,Ou X,Hauer-Jensen M

    更新日期:2003-11-01 00:00:00

  • Radiation protection by the ocimum flavonoids orientin and vicenin: mechanisms of action.

    abstract::In previous studies, flavonoids, orientin and vicenin, that were isolated from the leaf extract of Ocimum sanctum, were found to protect mice against radiation injury. Several flavonoids are known to be good antioxidants. Therefore, the effect of orientin and vicenin on radiation-induced lipid peroxidation in vivo and...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Uma Devi P,Ganasoundari A,Vrinda B,Srinivasan KK,Unnikrishnan MK

    更新日期:2000-10-01 00:00:00

  • Frequent chest X-ray fluoroscopy and breast cancer incidence among tuberculosis patients in Massachusetts.

    abstract::The incidence of breast cancer was determined in 4940 women treated for tuberculosis between 1925 and 1954 in Massachusetts. Among 2573 women examined by X-ray fluoroscopy an average of 88 times during lung collapse therapy and followed for an average of 30 years, 147 breast cancers occurred in contrast to 113.6 expec...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Boice JD Jr,Preston D,Davis FG,Monson RR

    更新日期:1991-02-01 00:00:00

  • Mechanisms of direct radiation damage in DNA, based on a study of the yields of base damage, deoxyribose damage, and trapped radicals in d(GCACGCGTGC)(2).

    abstract::Dose-response curves were measured for the formation of direct-type DNA products in X-irradiated d(GCACGCGTGC)(2)prepared as dry films and as crystalline powders. Damage to deoxyribose (dRib) was assessed by HPLC measurements of strand break products containing 3' or 5' terminal phosphate and free base release. Base d...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Swarts SG,Gilbert DC,Sharma KK,Razskazovskiy Y,Purkayastha S,Naumenko KA,Bernhard WA

    更新日期:2007-09-01 00:00:00

  • Delayed Growth Suppression and Radioresistance Induced by Long-Term Continuous Gamma Irradiation.

    abstract::Biological response to ionizing radiation depends not only on the type of radiation and dose, but also on the duration and dose rate of treatment. For a given radiation dose, the biological response may differ based on duration and dose rate. We studied the properties of two human cell lines, M059K glioma and U2OS ost...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Nakajima H,Furukawa C,Chang YC,Ogata H,Magae J

    更新日期:2017-08-01 00:00:00

  • Quantitative analysis of calcitonin gene-related peptide- and neuropeptide Y-immunoreactive nerve fibers in mesenteric blood vessels of rats irradiated with cobalt-60 gamma rays.

    abstract::The effects of 60Co gamma rays on calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and neuropeptide Y (NPY)-immunoreactive nerve fibers were examined in mesenteric blood vessels of rats. Using a free-floating immunostaining streptavidin-biotin peroxidase complex method combined with a nickel-enhancem...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Zhang CG,Cai WQ,Li Y,Huang WQ,Su HC

    更新日期:1998-01-01 00:00:00

  • Therapeutic success of boron neutron capture therapy (BNCT) mediated by a chemically non-selective boron agent in an experimental model of oral cancer: a new paradigm in BNCT radiobiology.

    abstract::The hypothesis of boron neutron capture therapy (BNCT) research has been that the short-range, high-linear energy transfer radiation produced by the capture of thermal neutrons by (10)B will potentially control tumor and spare normal tissue only if the boron compound selectively targets tumor tissue within the treatme...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Trivillin VA,Heber EM,Nigg DW,Itoiz ME,Calzetta O,Blaumann H,Longhino J,Schwint AE

    更新日期:2006-08-01 00:00:00

  • Chronic exposure to ionizing radiation as a tumor promoter in mouse skin.

    abstract::We have tested chronic exposure to 90Y beta radiation for its action as a complete tumor promoter, a stage I tumor promoter, or a stage II tumor promoter in SENCAR mouse skin. In skin initiated with a single application of 7,12,dimethylbenz[a]anthracene (DMBA, 10 nmol), chronic exposure to beta radiation as a complete...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Mitchel RE,Trivedi A

    更新日期:1992-02-01 00:00:00

  • A mortality study of employees of the nuclear industry in Oak Ridge, Tennessee.

    abstract::An analysis was conducted of 27,982 deaths among 106,020 persons employed at four Federal nuclear plants in Oak Ridge, Tennessee, between 1943 and 1985. The main objectives were to extend the evaluation of the health effects of employment in the nuclear industry in Oak Ridge to include most workers who were omitted fr...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Frome EL,Cragle DL,Watkins JP,Wing S,Shy CM,Tankersley WG,West CM

    更新日期:1997-07-01 00:00:00

  • Live cell imaging of heavy-ion-induced radiation responses by beamline microscopy.

    abstract::To study the dynamics of protein recruitment to DNA lesions, ion beams can be used to generate extremely localized DNA damage within restricted regions of the nuclei. This inhomogeneous spatial distribution of lesions can be visualized indirectly and rapidly in the form of radiation-induced foci using immunocytochemic...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Jakob B,Rudolph JH,Gueven N,Lavin MF,Taucher-Scholz G

    更新日期:2005-06-01 00:00:00

  • Optimizing and Profiling Prostaglandin E2 as a Medical Countermeasure for the Hematopoietic Acute Radiation Syndrome.

    abstract::Identification of medical countermeasures (MCM) to mitigate radiation damage and/or protect first responders is a compelling unmet medical need. The prostaglandin E2 (PGE2) analog, 16,16 dimethyl-PGE2 (dmPGE2), has shown efficacy as a radioprotectant and radiomitigator that can enhance hematopoiesis and ameliorate int...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Patterson AM,Wu T,Chua HL,Sampson CH,Fisher A,Singh P,Guise TA,Feng H,Muldoon J,Wright L,Plett PA,Pelus LM,Orschell CM

    更新日期:2020-12-10 00:00:00

  • Effect of low-level radiation on the death of male germ cells.

    abstract::Hormetic and adaptive responses induced by low-level radiation in hematopoietic and immune systems have been observed, as shown by stimulatory effects on cell growth and resistance to subsequent radiation-induced cytogenetic damage. However, in terms of cell death by apoptosis, the effects of low-level radiation are c...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Liu G,Gong P,Zhao H,Wang Z,Gong S,Cai L

    更新日期:2006-04-01 00:00:00

  • Cellular proto-oncogene expression following exposure of mice to gamma rays.

    abstract::Many studies have shown the importance of altered cellular proto-oncogene expression in contributing to changes in cell survival, cell transformation, and cell cycle progression. In these experiments we examined the effects of total-body exposure of BCF1 mice to gamma rays (3 Gy) in modulating expression of cellular o...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Anderson A,Woloschak GE

    更新日期:1992-06-01 00:00:00

  • Assessing the impact of ionizing radiation on aquatic invertebrates: a critical review.

    abstract::There is growing scientific, regulatory and public concern over anthropogenic input of radionuclides to the aquatic environment, especially given the issues surrounding existing nuclear waste, future energy demand and past or potential nuclear accidents. A change in the approach to how we protect the environment from ...

    journal_title:Radiation research

    pub_type: 杂志文章,评审


    authors: Dallas LJ,Keith-Roach M,Lyons BP,Jha AN

    更新日期:2012-05-01 00:00:00

  • The effect of 29 kV X rays on the dose response of chromosome aberrations in human lymphocytes.

    abstract::The induction of chromosome aberrations in human lymphocytes irradiated in vitro with X rays generated at a tube voltage of 29 kV was examined to assess the maximum low-dose RBE (RBE(M)) relative to higher-energy X rays or 60Co gamma rays. Since blood was taken from the same male donor whose blood had been used for pr...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Schmid E,Regulla D,Kramer HM,Harder D

    更新日期:2002-12-01 00:00:00

  • Hemodynamic Flow-Induced Mechanotransduction Signaling Influences the Radiation Response of the Vascular Endothelium.

    abstract::Hemodynamic shear stress is defined as the physical force exerted by the continuous flow of blood in the vascular system. Endothelial cells, which line the inner layer of blood vessels, sense this physiological force through mechanotransduction signaling and adapt to maintain structural and functional homeostasis. Hem...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Natarajan M,Aravindan N,Sprague EA,Mohan S

    更新日期:2016-08-01 00:00:00

  • Risk of chronic myeloid and acute leukemia mortality after exposure to ionizing radiation among workers at four U.S. nuclear weapons facilities and a nuclear naval shipyard.

    abstract::A nested case-control study was conducted among workers at five U.S. nuclear facilities to evaluate leukemia mortality risk (excluding chronic lymphocytic) from ionizing radiation using worksite doses and adjusting for potential confounding. Conditional logistic regression was used to estimate the relative risk (RR) o...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Schubauer-Berigan MK,Daniels RD,Fleming DA,Markey AM,Couch JR,Ahrenholz SH,Burphy JS,Anderson JL,Tseng CY

    更新日期:2007-02-01 00:00:00

  • Prevention of interphase death in rat thymocytes by bisulfite.

    abstract::The effect of sodium bisulfite, a specific inhibitor of chromatin proteolysis, on radiation damage in rat thymocytes in vitro was examined. Rat thymocytes irradiated with 1 kR X rays in vitro were incubated at 37 degrees C with 10 mM glucose for 4 to 6 hr. During that time development of interphase death as judged by ...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Ohyama H,Shimizu M,Yamada T

    更新日期:1983-07-01 00:00:00

  • Mortality among mound workers exposed to polonium-210 and other sources of radiation, 1944-1979.

    abstract::Polonium-210 is a naturally occurring radioactive element that decays by emitting an alpha particle. It is in the air we breathe and also a component of tobacco smoke. Polonium-210 is used as an anti-static device in printing presses and gained widespread notoriety in 2006 after the poisoning and subsequent death of a...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Boice JD Jr,Cohen SS,Mumma MT,Ellis ED,Cragle DL,Eckerman KF,Wallace PW,Chadda B,Sonderman JS,Wiggs LD,Richter BS,Leggett RW

    更新日期:2014-02-01 00:00:00

  • The structure of the guanine cation: ESR/ENDOR of cyclic guanosine monophosphate single crystals after X irradiation at 10 K.

    abstract::Following X irradiation of 3',5'-cyclic guanosine monophosphate single crystals at 10 K, several free radicals were trapped and detected by ESR/ENDOR/FSE spectroscopy. The two dominant species both have unpaired spin located on the guanine base. One is the product of net hydrogen atom loss from the exocyclic amino gro...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Hole EO,Sagstuen E,Nelson WH,Close DM

    更新日期:1992-01-01 00:00:00

  • Upper dose thresholds for radiation-induced adaptive response against cancer in high-dose-exposed, cancer-prone, radiation-sensitive Trp53 heterozygous mice.

    abstract::Trp53 heterozygous mice are radiation-sensitive and cancer-prone. Groups of 7-8-week-old female Trp53 heterozygous mice were exposed to 4 Gy of 60Co gamma radiation at high (0.5 Gy/min) or low (0.5 mGy/min) dose rate. Other groups received 10 or 100 mGy at low dose rate 24 h prior to the 4-Gy dose. Tumor frequency and...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Mitchel RE,Jackson JS,Carlisle SM

    更新日期:2004-07-01 00:00:00

  • Hypoadrenocorticism in beagles exposed to aerosols of plutonium-238 dioxide by inhalation.

    abstract::Hypoadrenocorticism, known as Addison's disease in humans, was diagnosed in six beagles after inhalation of at least 1.7 kBq/g lung of 238PuO2. Histological examination of adrenal gland specimens obtained at necropsy revealed marked adrenal cortical atrophy in all cases. Autoradiographs showed only slight alpha-partic...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Weller RE,Buschbom RL,Dagle GE,Park JF,Ragan HA,Watson CR

    更新日期:1996-12-01 00:00:00

  • Biological effects of radiofrequency fields: does modulation matter?

    abstract::This commentary considers modulation as a factor of potential biological importance in assessment of risk of radiofrequency (RF) energy emitted by communications systems and other technologies. Modulation introduces a spread of frequencies into a carrier waveform, but in nearly all cases this spread is small compared ...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Foster KR,Repacholi MH

    更新日期:2004-08-01 00:00:00

  • Effects of protons and HZE particles on glutamate transport in astrocytes, neurons and mixed cultures.

    abstract::Radiation-induced neurotoxicity is a well-characterized phenomenon. However, the underlying mechanism of this toxicity is poorly understood. In the central nervous system (CNS), excitotoxic mechanisms are implicated in many neurodegenerative disease processes. Pivotal to the excitotoxic pathway is dysfunction of gluta...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Sanchez MC,Nelson GA,Green LM

    更新日期:2010-12-01 00:00:00

  • Studies of the mortality of atomic bomb survivors, Report 14, 1950-2003: an overview of cancer and noncancer diseases.

    abstract::This is the 14th report in a series of periodic general reports on mortality in the Life Span Study (LSS) cohort of atomic bomb survivors followed by the Radiation Effects Research Foundation to investigate the late health effects of the radiation from the atomic bombs. During the period 1950-2003, 58% of the 86,611 L...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Ozasa K,Shimizu Y,Suyama A,Kasagi F,Soda M,Grant EJ,Sakata R,Sugiyama H,Kodama K

    更新日期:2012-03-01 00:00:00

  • Radiation enhances caspase 3 cleavage of Rad51 in BRCA2-defective cells.

    abstract::After DNA damage, caspases cleave and activate proteins involved in cell death by apoptosis but also cleave and inactivate proteins implicated in DNA repair. Here we report a rapid onset of Rad51 cleavage by caspase 3 in BRCA2-defective mouse and human cells. This rapid cleavage was reduced markedly by transfer of ful...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Brown ET,Robinson-Benion C,Holt JT

    更新日期:2008-05-01 00:00:00

  • Exposure to ≤15 cGy of 600 MeV/n 56Fe Particles Impairs Rule Acquisition but not Long-Term Memory in the Attentional Set-Shifting Assay.

    abstract::On future missions into deep space, astronauts will be required to work more autonomously than on previous missions, and thus their ability to perform executive functions could be critical to mission success. In this study, we determined the effect that ≤15 cGy of 600 MeV/n 56Fe particles has on attentional set-shifti...

    journal_title:Radiation research

    pub_type: 杂志文章


    authors: Jewell JS,Duncan VD,Fesshaye A,Tondin A,Macadat E,Britten RA

    更新日期:2018-12-01 00:00:00