Microcollimator for micrometer-wide stripe irradiation of cells using 20-30 keV X rays.

Abstract:

:Abstract Pataky, K., Villanueva, G., Liani, A., Zgheib, O., Jenkins, N., Halazonetis, D. J., Halazonetis, T. D. and Brugger, J. Microcollimator for Micrometer-Wide Stripe Irradiation of Cells Using 20-30 keV X Rays. Radiat. Res. 172, 252-259 (2009). The exposure of subnuclear compartments of cells to ionizing radiation is currently not trivial. We describe here a collimator for micrometer-wide stripe irradiation designed to work with conventional high-voltage X-ray tubes and cells cultured on standard glass cover slips. The microcollimator was fabricated by high-precision silicon micromachining and consists of X-ray absorbing chips with grooves of highly controlled depths, between 0.5-10 microm, along their surfaces. These grooves form X-ray collimating slits when the chips are stacked against each other. The use of this device for radiation biology was examined by irradiating human cells with X rays having energies between 20-30 keV. After irradiation, p53 binding protein 1 (53BP1), a nuclear protein that is recruited at sites of DNA double-strand breaks, clustered in lines corresponding to the irradiated stripes.

journal_name

Radiat Res

journal_title

Radiation research

authors

Pataky K,Villanueva G,Liani A,Zgheib O,Jenkins N,Halazonetis DJ,Halazonetis TD,Brugger J

doi

10.1667/RR1483.1

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

252-9

issue

2

eissn

0033-7587

issn

1938-5404

journal_volume

172

pub_type

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