Abstract：
:After a planned or unplanned radiation exposure, determination of absorbed dose has great clinical importance, informing treatment and triage decisions in the exposed individuals. Biodosimetry approaches allow for determination of dose in the absence of physical measurement apparatus. The current state-of-the-art biodosimetry method is based on the frequency of induced dicentric chromosomes in peripheral blood T cells, which is proportional to the absorbed radiation dose. Since dose-response curves used for obtaining absorbed dose for humans are based on data sourced from in vitro studies, a concerning discrepancy may be present in the reported dose. Specifically, T-cell survival after in vitro irradiation is much higher than that measured in humans in vivo and, in addition, is not dose dependent over some dose ranges. We hypothesized that these differences may lead to inappropriately inflated dicentric frequencies after in vitro irradiation when compared with in vivo irradiation of the same samples. This may lead to underestimation of the in vivo dose. To test this hypothesis, we employed the humanized mouse model, which allowed direct comparison of cell depletion and dicentric frequencies in human T cells irradiated in vivo and in vitro. The results showed similar dicentric chromosome induction frequencies measured in vivo and in vitro when assessed 24 h postirradiation despite the differences in cell survival. These results appear to validate the use of in vitro data for the estimation of the absorbed dose in human radiation biodosimetry.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Pujol-Canadell M,Young E,Smilenov Ldoi
10.1667/RR15283.1subject
Has Abstractpub_date
2019-05-01 00:00:00pages
439-446issue
5eissn
0033-7587issn
1938-5404pii
10.1667/RR15283.1journal_volume
191pub_type
杂志文章abstract：:Radiation-induced complications in bone and cartilage are of increasing concern due to potential long-term effects in cancer survivors. Healthy articular cartilage may be exposed to radiation during either chondrosarcoma treatment or in-field radiotherapy of tumors located in close proximity to articulation. Cartilage...
journal_title：Radiation research
pub_type： 杂志文章,评审
doi：10.1667/RR13928.1
更新日期：2015-02-01 00:00:00
abstract：:While the link between risk of leukemia and acute radiation exposure is well established for large doses received acutely, uncertainty remains around the translation of these risk estimates to occupational exposure scenarios where the doses are low and accumulated over time, possibly over many years. We present leukem...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15358.1
更新日期：2019-11-01 00:00:00
abstract：:The appearance of prostaglandins and thromboxane in mouse serum after X irradiation was observed by simultaneous quantitative analysis using gas chromatography/mass spectrometry/selected ion monitoring with stable isotope dilution methods. Mice of two strains (C57BL/CN Jcl and BALB/c) showed similar responses to X irr...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The threat of exposure to ionizing radiation from a nuclear reactor accident or deliberate terrorist actions is a significant public health concern. The lung is particularly susceptible to radiation-induced injury from external sources or inhalation of radioactive particles from radioactive fallout. Radiation-induced ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14686.1
更新日期：2017-11-01 00:00:00
abstract：:The A31-11 mouse BALB/3T3 cell system was adapted for the study of malignant transformation induced by 5.3 MeV alpha particles from a specially constructed 238Pu source. The biologic effects were compared to those of 220 kVp X rays. The alpha-radiation survival curve showed a D0 of 70 rad and a relative biological eff...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Cells from patients with ataxia telangiectasia (AT) are abnormal in their response to irradiation as judged by clonogenic survival and accumulation in G2 phase. The relationship of the results of these two assays, however, is still a matter of controversy. Flow cytometry was used to measure the distribution of cells i...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Metallothionein (MT), the synthesis of which can be induced by metalloelement administration, is a known radical scavenger. This study investigated the possible protective effect of MT against acute radiation injury. Manganese chloride (10 mg of manganese/kg) was administered intraperitoneally to male C3H/He mice 24 h...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Chinese hamster V79 cells grown for several hours in suspension culture form spheroids which are more resistant to killing by ionizing radiation than cells grown on petri dishes, a phenomenon known as the "contact effect." Previous results using the alkali-unwinding assay as a measure of DNA damage have implicated dif...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:While lifespan studies provide basic information for estimating the risk of ionizing radiation, findings on the effect of low-dose/low-dose-rate irradiation on the lifespan of mammals are controversial. Here we evaluate the effect of continuous exposure to low-dose-rate γ radiation on the lifespan of mice with acceler...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2977.1
更新日期：2013-06-01 00:00:00
abstract：:1-(2-Fluoro-4-iodo-2,4-dideoxy-beta-L-xylopyranosyl)-2-nitroimidazole (FIAZP) has been synthesized and labeled with radioiodine (125I). Radioiodinated FIAZP is one of a series of sugar-coupled 2-nitroimidazoles developed in our laboratory as probes for noninvasive scintigraphic assessment of tumor hypoxia. An in vivo ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Mathematical analysis of cell survival allows parameter estimation for radiobiological models and selection of an appropriate model. To our knowledge, no rigorous comparisons on the accuracy of various methods used for such analysis have been performed. In this study we compared three methods: 1. maximization of binom...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14195.1
更新日期：2016-03-01 00:00:00
abstract：:The effect of ionizing radiation on the survival of bovine aortic endothelial (BAE) cells was determined by the in vitro colony formation method. The BAE cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% calf serum, antibiotics, and growth factors obtained from the culture of mouse...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：:The effects of reoxygenation on repair of potentially lethal radiation damage were investigated using MG-63 human osteosarcoma cells in vitro. When exponentially growing MG-63 cells were cultured under hypoxic conditions for 24 h, cells stopped growing and remained at a low density. The hypoxic cells were then reoxyge...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:Studies were conducted to determine the effects of BeSO4 or X rays, alone and in combination, on cell cycle kinetics, cell killing, and the production of chromosome aberrations in Chinese hamster ovary (CHO) cells. The concentration of BeSO4 required to kill 50% of CHO cells exposed to BeSO4 for 20 h was determined to...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:Previously, we described the radiation-induced (RI) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) effect as the increased MTT metabolization at the intermediate dose region after the irradiation of an MCF-7/6 cell monolayer with an X-ray dose gradient. We wondered if the cell monolayer at the inte...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3070.1
更新日期：2013-04-01 00:00:00
abstract：:The efficiency of boron neutron capture therapy (BNCT) for malignant gliomas depends on the selective and absolute accumulation of (10)B atoms in tumor tissues. Only two boron compounds, BPA and BSH, currently can be used clinically. However, the detailed distributions of these compounds have not been determined. Here...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0501.1
更新日期：2007-01-01 00:00:00
abstract：:Induction of interphase death was examined in Chinese hamster ovary cells exposed to accelerated heavy ions (carbon, neon, argon and iron) of various linear energy transfers (LETs) (10-2000 keV/microm). The fraction of cells that underwent interphase death was determined by observing individual cells with time-lapse p...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:The statistical distribution of the number of ion pairs per ionizing event in a small volume simulating a tissue sphere was obtained by applying the Expectation-Maximization (EM) algorithm to experimental spectra measured by exposing a Rossi-type spherical proportional counter to gamma radiation. The normalized experi...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:These assessment of the consequences of irradiation on chromatin is complicated by endogenous nucleases. Isolation and prolonged storage of rat liver nuclei in buffers containing divalent metal ions activates these enzymes and promotes the degradation of chromatin. Irradiation of rat liver nuclei to dose levels of 20,...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:This laboratory previously demonstrated that hyperbaric oxygen and hyperbaric carbogen improved oxygenation in the R3230Ac tumor, but normobaric 100% O2 and carbogen did not. The current study assessed tumor growth after exposure to radiation plus either hyperbaric oxygen, carbogen or carbogen/nicotinamide and the rel...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:Radiation therapy for the treatment of thoracic cancers may be associated with radiation-induced heart disease (RIHD), especially in long-term cancer survivors. Mechanisms by which radiation causes heart disease are largely unknown. To identify potential long-term contributions of mitochondria in the development of ra...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR13452.1
更新日期：2014-03-01 00:00:00
abstract：:In light-ion radiation therapy, both the dose and the local energy spectrum, which is often characterized with the linear energy transfer (LET), must be considered. In treatment optimization, it is advantageous to use a radiobiological model that analytically accounts for both dose and LET for the ion type of interest...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2045.1
更新日期：2010-10-01 00:00:00
abstract：:The effect of X irradiation on the respiration of rat thymocytes was studied. An increase in the rate of O2 uptake was observed 1 h after cells were irradiated with doses of 6-10 Gy. The radiation-induced increase in respiration could be blocked by oligomycin, an inhibitor of mitochondrial ATP synthase, suggesting con...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:The hearts of mature male rats of the Wistar and Sprague-Dawley strains were locally irradiated with single doses of 17.5 and 20.0 Gy of X rays, respectively. These two dose levels had previously been shown to result in a comparable latent period between irradiation and the death of rats of these two strains from card...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Dose-response relationships for X-ray-induced reciprocal translocations in spermatogonial stem cells of mutant and wild-type mice were established by spermatocyte analysis many cell generations after irradiation. The mutants studied were Wv/+, the viable allele of dominant spotting in the heterozygous state, and Slcon...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:The presence of oxygen-deficient tumor cells is a critical issue in cancer therapy. To identify tumor hypoxia, tissue partial oxygen pressure (pO2) can be measured directly. The OxyLite system allows determination of pO2 in tumors and permits continuous measurements of pO2 at a fixed point. In this study, this system ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3602.1
更新日期：2006-09-01 00:00:00
abstract：:This experiment was performed to determine whether ionizing radiation is essential for maximum growth rate in a ciliated protozoan. When extraneous ionizing radiation was reduced to 0.15 mrad/day, the reproduction rate of Tetrahymena pyriformis was significantly less (P less than 0.01) than it was at near ambient leve...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：:The incidence of micronuclei was evaluated to assess the induction of an adaptive response to non-ionizing radiofrequency (RF) radiation in peripheral blood lymphocytes collected from five different human volunteers. After stimulation with phytohemagglutinin for 24 h, the cells were exposed to an adaptive dose of 900 ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1687.1
更新日期：2009-06-01 00:00:00
abstract：:The potential carcinogenic effects of internally deposited alpha-particle-emitting nuclides, notably plutonium, in the liver in humans are unknown but are of concern in relation to exposures from the nuclear industry. However, patients injected with the radiographic contrast medium Thorotrast are chronically exposed t...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Human cancers have multiple alterations in cell signaling pathways that promote resistance to cytotoxic therapy such as X rays. Parthenolide is a sesquiterpene lactone that has been shown to inhibit several pro-survival cell signaling pathways, induce apoptosis, and enhance chemotherapy-induced cell killing. We invest...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1128.1
更新日期：2007-12-01 00:00:00
