Abstract：
:To study the dynamics of protein recruitment to DNA lesions, ion beams can be used to generate extremely localized DNA damage within restricted regions of the nuclei. This inhomogeneous spatial distribution of lesions can be visualized indirectly and rapidly in the form of radiation-induced foci using immunocytochemical detection or GFP-tagged DNA repair proteins. To analyze faster protein translocations and a possible contribution of radiation-induced chromatin movement in DNA damage recognition in live cells, we developed a remote-controlled system to obtain high-resolution fluorescence images of living cells during ion irradiation with a frame rate of the order of seconds. Using scratch replication labeling, only minor chromatin movement at sites of ion traversal was observed within the first few minutes of impact. Furthermore, time-lapse images of the GFP-coupled DNA repair protein aprataxin revealed accumulations within seconds at sites of ion hits, indicating a very fast recruitment to damaged sites. Repositioning of the irradiated cells after fixation allowed the comparison of live cell observation with immunocytochemical staining and retrospective etching of ion tracks. These results demonstrate that heavy-ion radiation-induced changes in subnuclear structures can be used to determine the kinetics of early protein recruitment in living cells and that the changes are not dependent on large-scale chromatin movement at short times postirradiation.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Jakob B,Rudolph JH,Gueven N,Lavin MF,Taucher-Scholz Gdoi
10.1667/rr3374subject
Has Abstractpub_date
2005-06-01 00:00:00pages
681-90issue
6eissn
0033-7587issn
1938-5404pii
RR3374journal_volume
163pub_type
杂志文章abstract：:The effects of multiple-dose gamma irradiation on the shape of survival curves were studied with mouse C3H 10T1/2 cells maintained in contact-inhibited plateau phase. The dose-fractionation intervals included 3, 6, and 24 h. Following three fractionated doses (5 Gy per dose) of exposures, cells responded to further ir...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:The presence of low levels of oxygen may have profound effects on the cytotoxic activity of radiation, radiosensitizers, and bioreductive alkylating agents. As others have shown, low oxygen tensions may significantly alter rates of cellular and chemical oxygen consumption. When experiments are performed at very low ox...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours following irradiation, oxid...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-11-01 00:00:00
abstract：:An analytical expression is developed for calculating the average energy deposited by an ion in a volume with dimensions less than the range of the secondary electrons produced. The expression is obtained by including two additional terms in an energy-restricted linear-energy-transfer equation. The usual energy-restri...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:We examined the effects of X irradiation on activation of the promoter of the human HSP70B gene, which is activated only after heat shock. The HSP70B promoter was ligated to the bacterial beta-galactosidase (beta-gal) gene, and the reporter plasmid was transfected into NCI-H1299 carcinoma cells. The expression of the ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:Peroxidation of unconjugated polyunsaturated fatty acids such as linolenic acid proceeds through a free radical chain mechanism and is accompanied by the formation of conjugated dienes such as hydroperoxides. In an investigation of radiation-induced oxidation of aqueous linolenate, we have measured two indexes of pero...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)153[0201:eotfod]2.0.co;2
更新日期：2000-02-01 00:00:00
abstract：:Very low doses of ionizing radiation, 5 to 100 mGy, can induce adaptive responses characterized by elevation in cell survival and reduction in micronuclei formation. Utilizing these end points, RKO human colon carcinoma and transformed mouse embryo fibroblasts (MEF), wild-type or knockout cells missing TNF receptors 1...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3126.2
更新日期：2013-02-01 00:00:00
abstract：:Radiation of the esophagus of C3H/HeNsd mice with 35 or 37 Gy of 6 MV X rays induces significantly increased RNA transcription for interleukin 1 (Il1), tumor necrosis factor alpha (Tnf), interferon gamma inducing factor (Ifngr), and interferon gamma (Ifng). These elevations are associated with DNA damage that is detec...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0002:morice]2.0.co;2
更新日期：2001-01-01 00:00:00
abstract：:The filter elution technique using nondenaturing conditions is widely used to assay DNA double-strand break (DSB) induction and repair. It has been reported that in the measurement of strand breaks higher rates of elution and of initial rejoining are obtained at pH 9.6 compared to pH 7.2. In the present experiments ne...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Microwave radiation produces hyperthermia. The mammalian thermoregulatory system defends against changes in temperature by mobilizing diverse control mechanisms. Neurotransmitters play a major role in eliciting thermoregulatory responses. The involvement of adrenergic and muscarinic cholinergic receptors was investiga...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Gemcitabine (dFdCyd) shows broad antitumor activity in solid tumors in chemotherapeutic regimens or when combined with ionizing radiation (radiosensitization). While it is known that mismatches in DNA are necessary for dFdCyd radiosensitization, the critical event resulting in radiosensitization has not been identifie...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14443.1
更新日期：2016-11-01 00:00:00
abstract：:A rare T-cell subpopulation, CD4-8- alpha beta T cells, may be differentiated through a pathway (or pathways) different from the pathway(s) of conventional CD4+ or CD8+ T cells. In the present study, the frequencies of CD4-8-T cells in peripheral-blood alpha beta T cells in 409 atomic bomb survivors (160 estimated to ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:The radiation dose received by crew members in interplanetary space is influenced by the stage of the solar cycle. Using the recently developed models of the galactic cosmic radiation (GCR) environment and the energy-dependent radiation transport code, we have calculated the dose at 0 and 5 cm water depth; using a com...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:In a recent study (Int. J. Radiat. Oncol. Biol. Phys. 36, 635-639, 1996), 1H nuclear magnetic resonance (NMR) spectroscopy was used to demonstrate significant decreases in lactate levels after gamma irradiation of radiosensitive RIF-1 tumors in vitro. For comparison, we have examined the effects of gamma radiation on ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:We have developed a new detection method for measuring distributions of energy depositions from particles characterized by low linear energy transfers (LETs). In particular, we have developed a detection system to measure energy depositions produced by electrons and photons on nanometer scales. The detector is based u...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:We studied the LET and ion species dependence of the RBE for cell killing to clarify the differences in the biological effects caused by the differences in the track structure that result from the different energy depositions for different ions. Normal human skin fibroblasts were irradiated with heavy-ion beams such a...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3360
更新日期：2005-05-01 00:00:00
abstract：:This study evaluated the phagocytic and bactericidal activities of peripheral blood leukocytes from Hiroshima and Nagasaki atomic bomb survivors for Staphylococcus aureus. The data were analyzed by multiple linear regression for age, sex, radiation exposure, city of exposure, and neutrophil counts. No significant radi...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:The success of boron neutron capture therapy (BNCT) mainly depends on the boron concentration in the tumor and a high tumor/normal tissue (T/N) boron ratio or a high tumor/blood (T/B) boron ratio. Therefore, the effective enhancement of boron ratios is the first priority. Our study investigated whether a low-dose of γ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RADE-20-00102.1
更新日期：2021-01-29 00:00:00
abstract：:Plutonium is considered to be a carcinogen because it emits alpha particles that may result in the irradiation of stem cell population. In the present study we show that plutonium can also catalyze reactions that induce hydroxyl radicals in the absence of significant alpha-particle irradiation. Using the low specific ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:To understand the biophysical mechanism(s) underlying the induction of cell death by the decay of the Auger electron emitter iodine-125 in DNA, Chinese hamster V79 lung fibroblasts were labeled with 5-[(125)I]iodo-2'-deoxyuridine ((125)IdU) for two doubling times and frozen and stored at -135 degrees C in the presence...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2000)154[0326:todiiq]2.0.co;2
更新日期：2000-09-01 00:00:00
abstract：:To compare the genotoxic effects of high-LET ionizing radiation to those of low-LET radiation, we investigated the responses of human lymphoblastoid cells to DNA damage TK6 after treatment with either low-LET X rays or high-LET iron ions (1000 keV/microm). A highly localized distribution of gammaH2AX/RAD51 foci was ob...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0678:dccpih]2.0.co;2
更新日期：2002-12-01 00:00:00
abstract：:We investigated the effect of irradiation on the kinetic characteristics of amino acid and glucose transport, and the effect on the activity of the cell membrane-bound enzyme 5'-nucleotidase and on the receptor-mediated stimulation of cyclic adenosine monophosphate synthesis by prostaglandin E1. Irradiation inhibited ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:Cell-cell contact is thought to be critically involved in mechanisms leading to radioresistance. Here, we assessed the influence of confluent compared to subconfluent cell culture conditions and the radiosensitizing ability of E-cadherin inhibition alone or in combination with C225-mediated EGFR inhibition in human sq...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2855.1
更新日期：2012-09-01 00:00:00
abstract：:Low doses of ionizing radiation to cells and animals may induce adaptive responses that reduce the risk of cancer. However, there are upper dose thresholds above which these protective adaptive responses do not occur. We have now tested the hypothesis that there are similar lower dose thresholds that must be exceeded ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1414.1
更新日期：2008-12-01 00:00:00
abstract：:Angiogenesis defines the many steps involved in the growth and migration of endothelial cell-derived blood vessels. This process is necessary for the growth and metastasis of tumors, and considerable effort is being expended to find inhibitors of tumor angiogenesis. This usually involves screening of potential anti-an...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0171:rdaion]2.0.co;2
更新日期：2001-01-01 00:00:00
abstract：:In an effort to identify proteomic changes that may be useful for radiation biodosimetry, human cells of hematological origin were treated with ionizing radiation or mock-irradiated and then harvested at different times after treatment. Protein lysates were generated from these cells and evaluated by Western blotting ...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1638.1
更新日期：2009-05-01 00:00:00
abstract：:Hypoxic BP-8 murine sarcoma cells were exposed to misonidazole and/or radiation and the kinetics and extent of cell death were evaluated with the [125I]iododeoxyuridine-prelabeling assay. Cell death after treatment with lethal doses of misonidazole was rapid and essentially complete within 2 or 3 days after drug expos...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:Clustered damage sites other than double-strand breaks (DSBs) have the potential to contribute to deleterious effects of ionizing radiation, such as cell killing and mutagenesis. In the companion article (Semenenko et al., Radiat. Res. 164, 180-193, 2005), a general Monte Carlo framework to simulate key steps in the b...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3414
更新日期：2005-08-01 00:00:00
abstract：:Alpha-particle irradiation of cells damages not only the irradiated cells but also nontargeted bystander cells. It has been proposed that the bystander effect is caused by oxidants and free radicals generated by the radiation. Recent studies have shown that α(1)-microglobulin protects against cell damage caused by oxi...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2213.1
更新日期：2010-11-01 00:00:00
abstract：:Radon is recognized as a public health concern for indoor exposure. Precise quantification derived from occupational exposure in miners is still needed for estimating the risk and the factors that modify the dependence on cumulated exposure. The present paper reports on relationship between radon exposure and lung can...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR0848.1
更新日期：2008-02-01 00:00:00
