Radiation-induced changes in the cell membrane of cultured human endothelial cells.

Abstract:

:We investigated the effect of irradiation on the kinetic characteristics of amino acid and glucose transport, and the effect on the activity of the cell membrane-bound enzyme 5'-nucleotidase and on the receptor-mediated stimulation of cyclic adenosine monophosphate synthesis by prostaglandin E1. Irradiation inhibited the sodium-dependent amino acid transport by a reduced binding of the amino acid to the transport unit. The transport of glucose, which appeared to be a sodium-independent process, was temporarily stimulated by increased maximal velocity of the transport. No effect was found on the binding to the transport unit. Irradiation increased the 5'-nucleotidase activity and decreased the prostaglandin E1-stimulated cyclic adenosine monophosphate synthesis 48 h after exposure to 20 Gy. It is concluded that irradiation decreases sodium-dependent transport by impairment of the transport unit, does not impair a sodium-independent process, and has opposite effects on membrane-bound enzyme activity and a receptor-mediated process.

journal_name

Radiat Res

journal_title

Radiation research

authors

Vegt GB,Wassenaar AM,Kawilarang-de Haas EW,Schütte PP,van der Linden M,Di Bon-de Ruijter M,Boon A

subject

Has Abstract

pub_date

1985-12-01 00:00:00

pages

317-28

issue

3

eissn

0033-7587

issn

1938-5404

journal_volume

104

pub_type

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